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Rabbit Recombinant Monoclonal EAAT2 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-Fr and reacts with Mouse, Rat samples.

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Images

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-EAAT2 antibody [EPR19798] (AB313822), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-EAAT2 antibody [EPR19798] (AB313822), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-EAAT2 antibody [EPR19798] (AB313822), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-Fr
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

6 products for Alternative Version

Target data

Function

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7557442, PubMed:7698742, PubMed:9373176). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (By similarity). Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate (PubMed:9180080).

Alternative names

Eaat2, Glt1, Slc1a2, Excitatory amino acid transporter 2, GLT-1, Sodium-dependent glutamate/aspartate transporter 2, Solute carrier family 1 member 2

Recommended products

Rabbit Recombinant Monoclonal EAAT2 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-Fr and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19798
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

EAAT2 also known as excitatory amino acid transporter 2 or GLT-1 is a protein responsible for reuptake of glutamate from the synaptic cleft into glial cells preventing excitotoxicity. The protein has a mass of approximately 73 kDa and prominently expresses in the central nervous system particularly in astrocytes. By regulating extracellular glutamate levels EAAT2 helps maintain neurotransmitter balance critical for healthy neuron function.

Biological function summary

EAAT2 plays an important role in maintaining synaptic transmission and preventing overexcitation that can lead to neuronal damage. It is part of a transporter complex responsible for the movement of glutamate across the cell membrane working together with ions like sodium and potassium. Due to its significant role in the regulation of neurotransmitter levels in the brain EAAT2 is considered an important player in neural communication processes.

Pathways

EAAT2 is integral to the glutamatergic signaling pathway where its activity supports glutamate recycling and homeostasis. This pathway is important for normal cognitive functions such as learning and memory. EAAT2 works closely with other proteins such as EAAT1 in efforts to regulate glutamate concentrations in the extracellular space impacting synaptic strength and plasticity in the nervous system.

Associated diseases and disorders

Malfunctions of EAAT2 are associated with conditions like amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. In ALS EAAT2 dysfunction leads to accumulation of neurotoxic levels of glutamate causing motor neuron death. Similarly in Alzheimer’s disease impaired regulation of EAAT2 contributes to neurodegeneration through a related increase in neuronal excitotoxicity. Both conditions emphasize the critical importance of EAAT2 in neuroprotection and maintenance of normal brain function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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3 product images

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-EAAT2 antibody [EPR19798] (ab313822), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-EAAT2 antibody [EPR19798] (ab313822)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat striatum tissue labeling EAAT2 with ab313822 at 1/100 (5.0 µg/ml) (Green). Confocal image showing positive staining on rat striatum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab313822 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-EAAT2 antibody [EPR19798] (ab313822), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-EAAT2 antibody [EPR19798] (ab313822)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling EAAT2 with ab313822 at 1/100 (5.0 µg/ml) (Green). Negative control: confocal image showing no staining on mouse kidney (PMID: 11038258). The nuclear counterstain was DAPI (Blue). The section was incubated with ab313822 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-EAAT2 antibody [EPR19798] (ab313822), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-EAAT2 antibody [EPR19798] (ab313822)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse striatum tissue labeling EAAT2 with ab313822 at 1/100 (5.0 µg/ml) (Green). Confocal image showing positive staining on mouse striatum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab313822 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

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Product protocols

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