AB196186

Alexa Fluor® 647 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal EEA1 antibody - conjugated to Alexa Fluor® 647. Early Endosome marker. Suitable for Flow Cyt, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

ZFYVE2, EEA1, Early endosome antigen 1, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2

2 Images

Flow Cytometry - Alexa Fluor® 647 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (AB196186)

Flow Cyt

Unknown

Flow Cytometry - Alexa Fluor® 647 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (AB196186)

Overlay histogram showing Jurkat cells stained with ab196186 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab196186, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter.

This antibody gave a positive signal in Jurkat cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (AB196186)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (AB196186)

ab196186 staining EEA1 in Jurkat cells. The cells were fixed with 80% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196186 at 1/167 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker
Unconjugated

Anti-EEA1 antibody [EPR4245] - Early Endosome Marker
Carrier free

Anti-EEA1 antibody [EPR4245] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4245

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/alexa-fluor-647-rabbit-igg-monoclonal-epr25a-isotype-control-ab199093'>ab199093</a> - Rabbit monoclonal IgG (Alexa Fluor® 647), is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/167", "ICCIF-species-notes": "This product gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EEA1 (Early Endosome Antigen 1) is an important protein involved in endosomal trafficking with a molecular weight of approximately 162 kDa. It is a cytoplasmic protein expressed in various cell types acting mainly as an endosome marker. This protein contains a FYVE domain which facilitates binding to phosphatidylinositol 3-phosphate on endosomal membranes. EEA1 also interacts with Rab5 a small GTPase contributing to the fusion of early endosomes.

Biological function summary

EEA1 plays a significant role in early endosomal pathways facilitating endocytic vesicle fusion. EEA1 does not usually work alone; it functions as part of a larger complex involving other endosomal proteins. This protein acts as a tethering molecule which stabilizes the interaction of vesicles with endosomes. By anchoring vesicles EEA1 ensures the correct delivery and sorting of cargo within the cell which is important for cellular homeostasis and signaling.

Pathways

EEA1 contributes to the endocytic pathway and receptor recycling. It ensures proper functioning of the endocytic recycling of receptors such as the epidermal growth factor receptor (EGFR). EEA1-mediated tethering is vital for these pathways and it works closely with proteins like Rab5 which regulate endocytic trafficking. Through these interactions EEA1 affects cellular processes by controlling the flow of signaling receptors and other molecules within the cell.

EEA1 dysfunction is linked to neurodegenerative diseases and cancer. Abnormalities in its expression or function can disrupt endosomal trafficking leading to cellular stress and disease. For example in Alzheimer's disease impaired endosomal function involving EEA1 can contribute to amyloid-beta accumulation. In cancer altered EEA1 activity may contribute to aberrant receptor signaling which can promote unchecked cell division. The interplay with proteins like EGFR demonstrates EEA1's importance in maintaining cellular health and response mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

See full target information EEA1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

European journal of immunology 55:e202451192 PubMed40071689

2025

Vaginal Prevotella timonensis Bacteria Enhance HIV-1 Uptake and Differentially Affect Transmission by Distinct Primary Dendritic Cell Subsets.

Applications

Unspecified application

Species

Unspecified reactive species

Marleen Y van Smoorenburg,Ester B M Remmerswaal,Celia Segui-Perez,John L van Hamme,Karin Strijbis,Teunis B H Geijtenbeek

Nature biotechnology 43:52-62 PubMed38418648

2024

Morphological diversification and functional maturation of human astrocytes in glia-enriched cortical organoid transplanted in mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Meiyan Wang,Lei Zhang,Sammy Weiser Novak,Jingting Yu,Iryna S Gallina,Lynne L Xu,Christina K Lim,Sarah Fernandes,Maxim N Shokhirev,April E Williams,Monisha D Saxena,Shashank Coorapati,Sarah L Parylak,Cristian Quintero,Elsa Molina,Leonardo R Andrade,Uri Manor,Fred H Gage

EMBO reports 24:e57499 PubMed37401859

2023

ER-mitochondria contacts and cholesterol metabolism are disrupted by disease-associated tau protein.

Applications

Unspecified application

Species

Unspecified reactive species

Leonora Szabo,Nadia Cummins,Paolo Paganetti,Alex Odermatt,Andreas Papassotiropoulos,Celeste Karch,Jürgen Götz,Anne Eckert,Amandine Grimm

PLoS pathogens 18:e1010434 PubMed35390107

2022

Enterococcus faecalis alters endo-lysosomal trafficking to replicate and persist within mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ronni A G da Silva,Wei Hong Tay,Foo Kiong Ho,Frederick Reinhart Tanoto,Kelvin K L Chong,Pei Yi Choo,Alexander Ludwig,Kimberly A Kline

The Journal of biological chemistry 294:2744-2756 PubMed30573680

2018

TRAF3-interacting JNK-activating modulator promotes inflammation by stimulating translocation of Toll-like receptor 4 to lipid rafts.

Applications

Unspecified application

Species

Unspecified reactive species

Yehua Li,Jingmin Guan,Wenjia Wang,Chun Hou,Li Zhou,Jian Ma,Yunfeng Cheng,Shi Jiao,Zhaocai Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker

Flow Cytometry - Alexa Fluor® 647 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (AB196186)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (AB196186)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Primary Antibodies

Primary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise