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AB310056

Alexa Fluor® 647 Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471]

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Rabbit Recombinant Monoclonal eEF1A1/EF-Tu antibody - conjugated to Alexa Fluor® 647.

View Alternative Names

EEF1A, EF1A, LENG7, EEF1A1, Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, Eukaryotic elongation factor 1 A-1, Leukocyte receptor cluster member 7, EF-Tu, eEF1A-1

  • 578 PE

    PE Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471]

  • 660 APC

    APC Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471]

  • HRP

    HRP Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471]

  • Unconjugated

    Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471]

  • Carrier free

    Anti-eEF1A1/EF-Tu + eEF1A1 + EE1AL3/EEF1A1P5 antibody [EPR9471] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9471

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 6 continuous identical amino acids with eEF1A2. Cross-reactivity with this protein has not been confirmed experimentally.

EE1AL3/EEF1A1P5 it's a suspected pseudogene (https://www.ncbi.nlm.nih.gov/gene/158078). Our validation shows that this clone is able to detect Recombinant EF1AL3/EEF1A1P5 but endogenous protein expression is yet unknown.

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Product details

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The eEF1A1 also known as EF-Tu or eEF1A1-L3 is an elongation factor involved in protein synthesis. It facilitates the delivery of aminoacyl-tRNA to the A-site of the ribosome during translation. eEF1A1 exhibits a mass of approximately 50 kDa and is widely expressed in various tissues including the brain heart and skeletal muscle. Its expression level varies indicating that it plays a consistent role in translation machinery but may have tissue-specific functions.
Biological function summary

This elongation factor is important in promoting the accuracy and efficiency of protein translation. It interacts with several components of the protein synthesis machinery and forms part of a larger translational complex. eEF1A1 is involved in ensuring the correct amino acids are added during protein synthesis which affects protein structure and function. In addition to its canonical role it also engages in processes such as actin filament binding nuclear transport and other cell maintenance tasks.

Pathways

The activities of eEF1A1 intersect with key processes involved in cellular growth and proliferation. It plays a significant role in the mTOR signaling pathway which regulates cell growth by controlling protein synthesis. eEF1A1 also interacts with proteins like Rheb an important player in mTOR signaling. By participating in these pathways eEF1A1 links translation with broader regulatory networks that control cell metabolism.

EEF1A1 has connections to cancer and neurodegenerative diseases. Its elevated expression is often observed in several cancer types suggesting a role in tumorigenesis. Additionally misregulation of eEF1A1 may contribute to neurodegenerative disorders such as Alzheimer's disease where alterations in protein synthesis pathways are common. The protein's interaction with tau known in neuropathological conditions highlights its impact on neurodegeneration.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis (PubMed : 26593721, PubMed : 26651998, PubMed : 36123449, PubMed : 36264623, PubMed : 36638793). Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome (PubMed : 26593721, PubMed : 26651998, PubMed : 36123449, PubMed : 36264623, PubMed : 36638793). The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation (PubMed : 26593721, PubMed : 26651998, PubMed : 36123449, PubMed : 36264623). Also plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1 (PubMed : 17177976).. (Microbial infection) Required for the translation of viral proteins and viral replication during human coronavirus SARS-CoV-2 infection.
See full target information EEF1A1
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Product promise

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