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Rabbit Recombinant Monoclonal Elav-type RNA-binding protein ETR3 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (AB215232), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested
Mouse
Predicted
Rat
Predicted

Tested
Tested

Species
Human
Dilution info
1/100
Notes

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

1 product for Alternative Product

Target data

Function

RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle. Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB. Acts both as an activator and as a repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre-mRNA. Involved in the apoB RNA editing activity. Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury (By similarity). Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK. Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5. Binds preferentially to UG-rich sequences, in particular UG repeat and UGUU motifs. Binds to apoB mRNA, specifically to AU-rich sequences located immediately upstream of the edited cytidine. Binds AU-rich sequences in the 3'-UTR of COX2 mRNA (By similarity). Binds to an intronic RNA element responsible for the silencing of exon 21 splicing (By similarity). Binds to (CUG)n repeats (By similarity). May be a specific regulator of miRNA biogenesis. Binds to primary microRNA pri-MIR140 and, with CELF1, negatively regulates the processing to mature miRNA (PubMed:28431233).

Alternative names

BRUNOL3, CUGBP2, ETR3, NAPOR, CELF2, CUGBP Elav-like family member 2, CELF-2, Bruno-like protein 3, CUG triplet repeat RNA-binding protein 2, CUG-BP- and ETR-3-like factor 2, ELAV-type RNA-binding protein 3, Neuroblastoma apoptosis-related RNA-binding protein, RNA-binding protein BRUNOL-3, CUG-BP2, ETR-3, hNAPOR

Recommended products

Rabbit Recombinant Monoclonal Elav-type RNA-binding protein ETR3 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13374-2
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Elav-type RNA-binding protein ETR3 also known as CELF1 (CUGBP Elav-like family member 1) is involved in the regulation of pre-mRNA alternative splicing translation and stability. The protein has a mass of approximately 52 kDa. ETR3 is expressed in several tissues with higher levels found in the heart skeletal muscles and brain. These tissues are indicative of its important role in post-transcriptional mechanism. As a member of the ELAV (embryonic lethal abnormal vision)-like proteins ETR3 contains three RNA recognition motifs which mediate its binding to RNA.

Biological function summary

ETR3 influences various cellular processes by participating in RNA processing and nuclear-cytoplasmic transport. It forms part of ribonucleoprotein complexes that interact with target RNA impacting splicing decisions and gene expression levels. ETR3's activity is important for muscle and neuronal function helping modulate the expression of genes vital for muscle contraction and neuronal communication. Through its role as an RNA-binding regulator it can affect cell differentiation and growth.

Pathways

ETR3 plays significant roles in the regulation of alternative splicing and mRNA decay pathways. It interacts with several other splicing and mRNA degradation factors such as hnRNPH (heterogeneous nuclear ribonucleoprotein H) and PABPN1 (poly(A) binding protein nuclear 1). These interactions help control the turnover and translation of mRNA establishing a balance of protein synthesis important for maintaining cellular homeostasis. By influencing these pathways ETR3 impacts cardiac and neural-specific gene expression demonstrating its role in tissue-specific regulation.

Associated diseases and disorders

ETR3 is linked to myotonic dystrophy and cardiac arrhythmias. ETR3 misregulation leads to disruptions in alternative splicing of specific mRNAs contributing to the pathophysiology of these conditions. The relationship between ETR3 and proteins such as MBNL1 (Muscleblind-like splicing regulator 1) in myotonic dystrophy is particularly significant. Both proteins regulate alternative splicing and their misregulated expression in disease highlights an important area for potential therapeutic interventions. The association of ETR3 with such disorders highlights its relevance in maintaining normal cellular and physiological functions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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1 product image

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab215232), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab215232)

    ab215232 staining Elav-type RNA-binding protein ETR3 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab215232 at 1/100 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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