Rabbit Recombinant Monoclonal ENT2 antibody - conjugated to Alexa Fluor® 647.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Target Binding Affinity | Reactivity Expected | Dilution info - | Notes - |
Application Antibody Labelling | Reactivity Expected | Dilution info - | Notes - |
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Bidirectional uniporter involved in the facilitative transport of nucleosides and nucleobases, and contributes to maintaining their cellular homeostasis (PubMed:10722669, PubMed:12527552, PubMed:12590919, PubMed:16214850, PubMed:21795683, PubMed:9396714, PubMed:9478986). Functions as a Na(+)-independent, passive transporter (PubMed:9478986). Involved in the transport of nucleosides such as inosine, adenosine, uridine, thymidine, cytidine and guanosine (PubMed:10722669, PubMed:12527552, PubMed:12590919, PubMed:16214850, PubMed:21795683, PubMed:9396714, PubMed:9478986). Also able to transport purine nucleobases (hypoxanthine, adenine, guanine) and pyrimidine nucleobases (thymine, uracil) (PubMed:16214850, PubMed:21795683). Involved in nucleoside transport at basolateral membrane of kidney cells, allowing liver absorption of nucleoside metabolites (PubMed:12527552). Mediates apical nucleoside uptake into Sertoli cells, thereby regulating the transport of nucleosides in testis across the blood-testis-barrier (PubMed:23639800). Mediates both the influx and efflux of hypoxanthine in skeletal muscle microvascular endothelial cells to control the amount of intracellular hypoxanthine available for xanthine oxidase-mediated ROS production (By similarity). Isoform 3. Non functional nucleoside transporter protein for adenosine or thymidine transport. Does not express on cell membrane.
DER12, ENT2, HNP36, SLC29A2, Equilibrative nucleoside transporter 2, hENT2, 36 kDa nucleolar protein HNP36, Delayed-early response protein 12, Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter, Solute carrier family 29 member 2, Equilibrative NBMPR-insensitive nucleoside transporter
Rabbit Recombinant Monoclonal ENT2 antibody - conjugated to Alexa Fluor® 647.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
May be unsuitable with mouse in WB.
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
ENT2 also known as Equilibrative Nucleoside Transporter 2 facilitates the transport of nucleosides across cellular membranes. It weighs approximately 56 kDa. The transporter is widely expressed in various tissues including the brain heart and placenta. By allowing the movement of nucleosides ENT2 plays a fundamental role in nucleoside salvage an important process for maintaining cellular nucleotide pools especially in energy-demanding tissues.
ENT2 participates in the regulation of intracellular nucleoside levels which impacts DNA and RNA synthesis. It operates individually and does not form part of a larger protein complex allowing it to specifically affect the transport rates based on cellular requirements. This role supports essential cellular processes like cell growth and division highlighting ENT2 as an important regulator in genomic stability and cellular homeostasis.
Four words not connected to nucleoside metabolism are vital choices. ENT2 plays major roles in the purine and pyrimidine salvage pathways. These pathways involve essential enzymes that facilitate the recycling of nucleosides for nucleotide synthesis. Related proteins include ENT1 another member of the equilibrative nucleoside transporter family which shares functional similarities in nucleoside transport across membranes and influences the dynamics of these pathways.
Four words unrelated to neurological conditions deserve attention. Dysfunction of ENT2 links to conditions like myocardial ischemia and neurodegenerative disorders. In these conditions ENT2's role in regulating nucleoside availability directly impacts cellular stress responses. For instance its interaction with nucleoside transport can modulate the extracellular adenosine levels influencing the purinergic signaling pathways. In cardiac tissues altered ENT2 function affects the availability of nucleosides during ischemic events potentially exacerbating tissue damage while in neurodegenerative contexts its regulation of nucleoside levels can impact neuron survival and function potentially involving proteins like adenosine deaminase.
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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