AB196149

Alexa Fluor® 647 Anti-ERG antibody [EPR3864]

Name: Alexa Fluor® 647 conjugated Anti-ERG antibody [EPR3864] (ab196149) | Abcam
Brand: Abcam Limited
SKU: AB196149
Price: 680 USD
Availability: InStock
Rating: 4 (3 reviews)

RabMAb
Recombinant
What is this?

(3 Reviews)

(31 Publications)

Anti-ERG antibody [EPR3864] - Alexa Fluor® 647 conjugated (ab196149) is a rabbit recombinant monoclonal antibody detecting ERG in ICC/IF. Suitable for Human.

- Clone EPR3864 is the most cited clone to ERG
- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications

View Alternative Names

Transcriptional regulator ERG, Transforming protein ERG, ERG

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (AB196149)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (AB196149)

ab196149 staining ERG in THP-1 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196149 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

HRP

HRP Anti-ERG antibody [EPR3864]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-ERG antibody [EPR3864]
578 PE

PE Anti-ERG antibody [EPR3864]
660 APC

APC Anti-ERG antibody [EPR3864]
Unconjugated

Anti-ERG antibody [EPR3864]
Carrier free

Anti-ERG antibody [EPR3864] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3864

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in THP-1 cells fixed with 4% formaldehyde (10 min)</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (ab196149) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Trusted by the scientific community
Anti-ERG [EPR3864] Alexa Fluor® 647 (ab196149) was first used in a scientific publication in 2015 and has been cited over 20 times in peer-reviewed journals.

Related products
Antibody clone EPR3864 is also available pre-conjugated to a variety of labels for your convenience – Anti-ERG Alexa Fluor® 647 [EPR3864] (ab196149).

Other related products
We have a range of other formats of antibody clone [EPR3864] also available for your convenience: ab92513, HRP - ab195514, Alexa Fluor® 647 - ab196149, Alexa Fluor® 488 - ab196374, ab214341, Carrier free - ab214796, Carrier free - ab275331, PE - ab314261, APC - ab315146

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERG or ETS-related gene is a transcription factor that belongs to the ETS (E-26 transformation specific) family. The ERG protein plays an important role in gene regulation involved in cell growth and differentiation. ERG has a molecular mass of approximately 54 kDa. It is expressed in a variety of tissues including the prostate endothelial cells and hematopoietic cells. Researchers often study ERG protein localization through techniques such as ERG IHC to better understand its expression across different tissues for example in the ERG color sections that highlight specific cellular contexts.

Biological function summary

ERG regulates processes critical for normal cellular functions such as proliferation apoptosis and differentiation. It functions as part of a larger transcriptional regulatory complex and interacts with other proteins to exert its effects on target genes. The ERG protein can affect angiogenesis and limb development through its regulation of vascular endothelial cells. By controlling such important cellular processes ERG influences both normal physiological functions and abnormal conditions when dysregulated.

Pathways

ERG is highly significant in the MAPK/ERK signaling pathway which is involved in cell division and survival. ERG interacts with other signaling proteins such as FLI1 and RUNX1 affecting cellular responses and contributing to the regulation of gene expression essential for development and homeostasis. Within the hematopoietic context ERG participates in the regulation of pathways that maintain stem cell populations making it important for normal blood cell development.

ERG is prominently implicated in prostate cancer where gene fusions involving ERG lead to its overexpression and contribute to malignancy. The abnormal expression of ERG in endothelial cells can also have a role in vascular disorders. ERG's interplay with proteins like TMPRSS2 often seen in gene fusions in prostate cancer underlines its role in oncogenesis. Understanding how ERG regulates these pathways and its interactions with other proteins helps in developing targeted therapies for disorders involving ERG dysregulation.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.

See full target information ERG

style

left-column

Publications (31)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation : PubMed41046267

2025

Neuronal MCT2 promotes angiogenesis via lactate in the developing mouse neocortex.

Applications

Unspecified application

Species

Unspecified reactive species

Daehoon Lee,Anika Wu,Lingling Yao,Shreya Satish,Lin Mei,Wen-Cheng Xiong

Nature genetics 57:2177-2191 PubMed40858905

2025

ERG-driven prostate cancer initiation is cell-context dependent and requires KMT2A and DOT1L.

Applications

Unspecified application

Species

Unspecified reactive species

Weiran Feng,Erik Ladewig,Matthew Lange,Nazifa Salsabeel,Huiyong Zhao,Young Sun Lee,Anuradha Gopalan,Hanzhi Luo,Wenfei Kang,Ning Fan,Eric Rosiek,Elisa de Stanchina,Yu Chen,Brett S Carver,Christina S Leslie,Charles L Sawyers

Science advances 11:eads0505 PubMed40238886

2025

Amyotrophic lateral sclerosis and frontotemporal dementia mutation reduces endothelial TDP-43 and causes blood-brain barrier defects.

Applications

Unspecified application

Species

Unspecified reactive species

Ashok Cheemala,Amy L Kimble,Emily N Burrage,Stephen B Helming,Jordan D Tyburski,Nathan K Leclair,Omar M Omar,Aamir R Zuberi,Melissa Murphy,Evan R Jellison,Bo Reese,Xiangyou Hu,Cathleen M Lutz,Riqiang Yan,Patrick A Murphy

Communications biology 8:22 PubMed39779980

2025

A perfusion-independent high-throughput method to isolate liver sinusoidal endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Babin-Ebell Gonçalves,Yifang Mao,Tinja Baljkas,Felix Wiedmann,Larissa Eis,Franziska Pilz,Manuel Winkler,Sina W Kürschner-Zacharias,Marlene Hoffarth,Charlotta Funaya,Réza Shahidi,Cyrill Géraud,Chi-Chung Wu,Constanze Schmidt,Sergij Goerdt,Philipp-Sebastian Reiners-Koch,Mahak Singhal

Cardiovascular research 120:1967-1984 PubMed39308243

2024

A non-genetic model of vascular shunts informs on the cellular mechanisms of formation and resolution of arteriovenous malformations.

Applications

Unspecified application

Species

Unspecified reactive species

Marie Ouarné,Andreia Pena,Daniela Ramalho,Nadine V Conchinha,Tiago Costa,Romain Enjalbert,Ana M Figueiredo,Marta Pimentel Saraiva,Yulia Carvalho,Miguel O Bernabeu,Lenka Henao Misikova,S Paul Oh,Cláudio A Franco

Nature 628:863-871 PubMed38570687

2024

A brain-specific angiogenic mechanism enabled by tip cell specialization.

Applications

Unspecified application

Species

Unspecified reactive species

Giel Schevenels,Pauline Cabochette,Michelle America,Arnaud Vandenborne,Line De Grande,Stefan Guenther,Liqun He,Marc Dieu,Basile Christou,Marjorie Vermeersch,Raoul F V Germano,David Perez-Morga,Patricia Renard,Maud Martin,Michael Vanlandewijck,Christer Betsholtz,Benoit Vanhollebeke

Frontiers in cell and developmental biology 11:1256127 PubMed38020883

2023

Capillary pruning couples tissue perfusion and oxygenation with cardiomyocyte maturation in the postnatal mouse heart.

Applications

Unspecified application

Species

Unspecified reactive species

Ricardo Santamaría,Javier Cruz-Caballero,Polyxeni Gkontra,Alberto Jiménez-Montiel,Cristina Clemente,Juan A López,María Villalba-Orero,Jesús Vázquez,Andreas Hutloff,Enrique Lara-Pezzi,Alicia G Arroyo

Brain, behavior, & immunity - health 34:100684 PubMed37822873

2023

Universal method for the isolation of microvessels from frozen brain tissue: A proof-of-concept multiomic investigation of the neurovasculature.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Wakid,Daniel Almeida,Zahia Aouabed,Reza Rahimian,Maria Antonietta Davoli,Volodymyr Yerko,Elena Leonova-Erko,Vincent Richard,René Zahedi,Christoph Borchers,Gustavo Turecki,Naguib Mechawar

Nature communications 14:1929 PubMed37024491

2023

MEK inhibition reduced vascular tumor growth and coagulopathy in a mouse model with hyperactive GNAQ.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Schrenk,Lindsay J Bischoff,Jillian Goines,Yuqi Cai,Shruti Vemaraju,Yoshinobu Odaka,Samantha R Good,Joseph S Palumbo,Sara Szabo,Damien Reynaud,Catherine D Van Raamsdonk,Richard A Lang,Elisa Boscolo

eLife 12: PubMed36989130

2023

Nuclear SUN1 stabilizes endothelial cell junctions via microtubules to regulate blood vessel formation.

Applications

Unspecified application

Species

Unspecified reactive species

Danielle B Buglak,Pauline Bougaran,Molly R Kulikauskas,Ziqing Liu,Elizabeth Monaghan-Benson,Ariel L Gold,Allison P Marvin,Andrew Burciu,Natalie T Tanke,Morgan Oatley,Shea N Ricketts,Karina Kinghorn,Bryan N Johnson,Celia E Shiau,Stephen Rogers,Christophe Guilluy,Victoria L Bautch

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-ERG antibody [EPR3864]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (AB196149)

Related conjugates and formulations (6)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (31)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise