Alexa Fluor® 647 Anti-Estrogen Receptor alpha antibody [EPR4097]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Estrogen Receptor alpha antibody [EPR4097] (AB205851)

Immunohistochemical analysis of paraffin-embedded human breast cancer labeling Estrogen Receptor alpha with ab205851 at 1/50 (10 ug/ml) dilution.

Nuclear staining on human breast cancer. The primary antibody was incubated overnight at 4°C (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Estrogen Receptor alpha antibody [EPR4097] (AB205851)

ab205851 staining Estrogen Receptor alpha in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab205851 at a 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in MCF7 cells fixed with 100% methanol (5 min)

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Estrogen Receptor alpha antibody [EPR4097] (AB205851)

Immunohistochemical analysis of paraffin-embedded human tonsil labeling Estrogen Receptor alpha with ab205851 at 1/50 (10 ug/ml) dilution.

Negative control : no staining on human tonsil. The primary antibody was incubated overnight at 4°C (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).