AB204467

Alexa Fluor® 647 Anti-F4/80 antibody [F4/80]

Name: Anti-F4/80 Alexa Fluor® 647 antibody [F4/80] (ab204467) IgG2b | Abcam
Brand: Abcam Limited
SKU: AB204467

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(18 Publications)

Rat Monoclonal F4/80 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Mouse samples. Cited in 18 publications.

View Alternative Names

Emr1, Gpf480, Adgre1, Adhesion G protein-coupled receptor E1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-F4/80 antibody [F4/80] (AB204467)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-F4/80 antibody [F4/80] (AB204467)

ab204467 staining F4/80 in Raw264.7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204467 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-F4/80 antibody [F4/80]
Unconjugated

Anti-F4/80 antibody [F4/80] - Macrophage Marker

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

F4/80

Isotype

IgG2b

Light chain type

kappa

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Mouse

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p>This product gave a positive signal in MCF7 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p>" } } }

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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

F4/80 also known as Emr1 (Epidermal growth factor module-containing mucin-like hormone receptor 1) is a well-characterized surface protein with a mass of approximately 160 kDa. This protein is recognized as a marker for murine macrophages. F4/80 is heavily expressed on cells of the monocyte and macrophage lineage particularly in tissue-resident macrophages found in organs like the liver spleen and lung. Its expression is vital for identifying macrophages in various types of immunological studies including F4/80 staining and macrophage marker analysis.

Biological function summary

F4/80 plays a role in modulating the immune response by interacting with other cell surface molecules. While not part of a larger protein complex F4/80 influences macrophage functions such as phagocytosis and cytokine production. Its expression contributes to the regulation of tissue homeostasis and inflammation. The protein is critical in maintaining the macrophage population required for reliable immune surveillance and response to pathogens.

Pathways

F4/80 is an integral part of the immunological and inflammatory signaling pathways. It interacts with TLRs (Toll-like receptors) and other cell surface molecules that sense microbial components therefore helping in the initiation of innate immune responses. F4/80 is connected to proteins like cytokine receptors which play roles in mediating and resolving inflammatory signals important for maintaining balanced immune activity.

F4/80 is associated with conditions like chronic inflammation and autoimmune disorders. Its expression levels can often correlate with disease state impacting diseases such as rheumatoid arthritis and liver fibrosis. In these conditions F4/80 interacts with proteins like TNF-alpha and IL-1beta which are involved in inflammatory processes. Understanding F4/80's role can help uncover therapeutic targets aimed at modulating macrophage functions to treat these disorders effectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

See full target information Adgre1

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 53:102310 PubMed39929064

2025

CSF3 promotes colorectal cancer progression by activating p65/NF-κB signaling pathway and inducing an immunosuppressive microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Junfeng Xu,Na Li,Hui Xie,Changwei Duan,Xingchen Liao,Ruoran Li,Heng Zhang,Yuanming Pan,Xianzong Ma,Shuwen Du,Jianqiu Sheng,Xin Wang,Lang Yang,Peng Jin

International journal of molecular sciences 25: PubMed39063080

2024

The Contrasting Effects of Two Distinct Exercise Training Modalities on Exhaustive Exercise-Induced Muscle Damage in Mice May Be Associated with Alterations in the Gut Microbiota.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Zhang,Cong Wang,Hedong Lang,Hongtao Yu,Min Zhou,Xin Rao,Qianyong Zhang,Long Yi,Jundong Zhu,Mantian Mi

Nature 628:612-619 PubMed38509366

2024

Venous-plexus-associated lymphoid hubs support meningeal humoral immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Zachary Fitzpatrick,Nagela Ghabdan Zanluqui,Jared S Rosenblum,Zewen Kelvin Tuong,Colin Y C Lee,Vikram Chandrashekhar,Maria Luciana Negro-Demontel,Andrew P Stewart,David A Posner,Monica Buckley,Kieren S J Allinson,Panagiotis Mastorakos,Prashant Chittiboina,Dragan Maric,Danielle Donahue,Adel Helmy,Tamara Tajsic,John R Ferdinand,Anais Portet,Ana Peñalver,Eleanor Gillman,Zhengping Zhuang,Menna R Clatworthy,Dorian B McGavern

International journal of molecular sciences 24: PubMed37047475

2023

SMTP-44D Inhibits Atherosclerotic Plaque Formation in Apolipoprotein-E Null Mice Partly by Suppressing the AGEs-RAGE Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Michishige Terasaki,Keita Shibata,Yusaku Mori,Tomomi Saito,Takanori Matsui,Makoto Ohara,Tomoyasu Fukui,Keiji Hasumi,Yuichiro Higashimoto,Koji Nobe,Sho-Ichi Yamagishi

Inflammation research : official journal of the European Histamine Research Society ... [et al.] 72:531-540 PubMed36633616

2023

TCR repertoire landscape reveals macrophage-mediated clone deletion in endotoxin tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Juanjuan Zhao,Li Jia,YiJing Tao,Xu Zhao,Jing Yang,Yanxin Lu,Yaping Yan,Ling Mao,Lin Hu,Jia Lu,MengMeng Guo,Chao Chen,Ya Zhou,Zhenke Wen,Zhixu He,Lin Xu

British journal of pharmacology 180:1168-1185 PubMed36495259

2022

Curaxin CBL0137 inhibits endothelial inflammation and atherogenesis via suppression of the Src-YAP signalling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Huanyu Ding,Minchun Jiang,Chi Wai Lau,Jianfang Luo,Andrew M Chan,Li Wang,Yu Huang

iScience 25:104660 PubMed35845169

2022

Group 3 innate lymphocytes make a distinct contribution to type 17 immunity in bladder defence.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra M Riding,Kevin W Loudon,Andrew Guo,John R Ferdinand,Laurence S C Lok,Nathan Richoz,Andrew Stewart,Tomas Castro-Dopico,Zewen Kelvin Tuong,Remi Fiancette,Georgina S Bowyer,Aaron Fleming,Eleanor S Gillman,Ondrej Suchanek,Krishnaa T Mahbubani,Kourosh Saeb-Parsy,David Withers,Gordan Dougan,Simon Clare,Menna R Clatworthy

Cell reports 37:110132 PubMed34936871

2021

Resolving the immune landscape of human prostate at a single-cell level in health and cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zewen Kelvin Tuong,Kevin W Loudon,Brendan Berry,Nathan Richoz,Julia Jones,Xiao Tan,Quan Nguyen,Anne George,Satoshi Hori,Sarah Field,Andy G Lynch,Katarzyna Kania,Paul Coupland,Anne Babbage,Richard Grenfell,Tristan Barrett,Anne Y Warren,Vincent Gnanapragasam,Charlie Massie,Menna R Clatworthy

Clinical cancer research : an official journal of the American Association for Cancer Research 28:327-337 PubMed34615724

2021

F-FMISO PET Imaging Identifies Hypoxia and Immunosuppressive Tumor Microenvironments and Guides Targeted Evofosfamide Therapy in Tumors Refractory to PD-1 and CTLA-4 Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Kirsten M Reeves,Patrick N Song,Allyson Angermeier,Deborah Della Manna,Yufeng Li,Jianbo Wang,Eddy S Yang,Anna G Sorace,Benjamin M Larimer

Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology head and neck surgery 34:486-491 PubMed32842175

2020

[The distribution of perivascular-resident cells in blood-labyrinth barrier observed with two-photon fluorescence microscope and Imaris deconvolution].

Applications

Unspecified application

Species

Unspecified reactive species

Ying Jiang,Hongbing Yao,Junhong Chen,Jie Zhang,Yufang Rao,Kai Chen,Yuedi Tang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Alexa Fluor® 647 Anti-F4/80 antibody [F4/80]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-F4/80 antibody [F4/80] (AB204467)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

Product promise