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Rabbit Recombinant Monoclonal FCRL4 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-FCRL4 antibody [EPR21961] (AB320114), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-FCRL4 antibody [EPR21961] (AB320114), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-FCRL4 antibody [EPR21961] (AB320114), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

May function as an inhibitor of the B-cell receptor signaling. May function in the B-cell-mediated immune response.

Alternative names

CD307d, FCRH4, IFGP2, IRTA1, FCRL4, Fc receptor-like protein 4, FcR-like protein 4, FcRL4, Fc receptor homolog 4, IFGP family protein 2, Immune receptor translocation-associated protein 1, FcRH4, hIFGP2

Recommended products

Rabbit Recombinant Monoclonal FCRL4 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR21961
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

FCRL4 also known as Fc receptor-like 4 is a transmembrane protein with a molecular mass of about 70 kDa. It belongs to a family of receptors similar to Fc receptors and is expressed mainly on a subset of B cells particularly on those in tonsils and mucosal tissues. This receptor is relevant because it modulates immune responses and can influence how B cells react to environmental antigens. FCRL4 expression differs from its family members suggesting a specialized function within the immune system.

Biological function summary

FCRL4 contributes to the regulation of B cell activation and differentiation. FCRL4 has been identified to form complexes with other proteins at the cellular membrane influencing signaling cascades that dampen B cell receptor signaling. This control over signaling pathways is critical for maintaining immune homeostasis and preventing overactive immune responses which can lead to autoimmune conditions. By acting through these complexes FCRL4 helps determine B cell fate during immune responses.

Pathways

FCRL4 is involved in immune regulatory pathways associated with lymphocyte activation. It intersects with the B cell receptor signaling pathway and modulates the interaction between B cell immunological synapse components. FCRL4 interacts with proteins like CD307d within these pathways serving a regulatory role in limiting excessive B cell activation. The control exerted by FCRL4 within these pathways is essential for preventing unnecessary immune activation that could be harmful.

Associated diseases and disorders

FCRL4 holds significance in chronic infections and autoimmune disorders. Abnormal expression or malfunction of FCRL4 associates with conditions such as Sjögren's syndrome where inappropriate immune function affects secretory glands. In chronic infections FCRL4 expression identifies exhausted B cell populations that are less responsive to antigenic stimulation affecting the body's ability to fight infections. CD307d often links to FCRL4 in such cases where they may together alter disease progression by affecting B cell function in disease settings.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-FCRL4 antibody [EPR21961] (ab320114), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-FCRL4 antibody [EPR21961] (ab320114)

    Immunohistochemical analysis of paraffin-embedded Human non-Hodgkin's lymphoma tissue labeling FCRL4 with ab320114 at 1/50 (10.0 ug/ml) dilution.

    Cytoplasmic and membranous staining on human non-Hodgkin's lymphoma.

    The section was incubated with ab320114 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-FCRL4 antibody [EPR21961] (ab320114), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-FCRL4 antibody [EPR21961] (ab320114)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling FCRL4 with ab320114 at 1/50 (10.0 ug/ml) dilution.

    Negative control: no staining on human skeletal muscle.

    The section was incubated with ab320114 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-FCRL4 antibody [EPR21961] (ab320114), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-FCRL4 antibody [EPR21961] (ab320114)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling FCRL4 with ab320114 at 1/50 (10.0 ug/ml) dilution.

    Cytoplasmic and membranous staining on human tonsil.

    The section was incubated with ab320114 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com