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AB239341

Alexa Fluor® 647 Anti-FOXP3 antibody [3G3]

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Mouse Monoclonal FOXP3 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FOXP3.

View Alternative Names

IPEX, JM2, FOXP3, Forkhead box protein P3, Scurfin

2 Images
Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-FOXP3 antibody [3G3] (AB239341)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-FOXP3 antibody [3G3] (AB239341)

Flow Cytometry (Intracellular) analysis of human peripheral whole blood labeling FoxP3 with ab239341 (concentration in sample 3 μg/ml).

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-FOXP3 antibody [3G3] (AB239341)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-FOXP3 antibody [3G3] (AB239341)

Flow Cytometry multicolor surface staining of human CD4 positive lymphocytes using PE conjugated anti-human CD25 antibody (20 μl reagent / 100 μl of peripheral whole blood) and intracellular staining of human CD4 positive lymphocytes using ab239341 (concentration in sample 3 μg/ml).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3G3

Isotype

IgG1

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Recombinant Full Length Protein corresponding to Human FOXP3.

Q9BZS1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1-5 g/mL", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purified antibody is conjugated with Alexa Fluor® 647 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage buffer
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXP3 also recognized as Forkhead box P3 is a transcription factor critical for immune system function. It weighs approximately 47 kDa. FOXP3 is mainly expressed in regulatory T cells (Tregs) which play a role in maintaining immune tolerance. Scientists primarily identify its presence in the nucleus where it facilitates transcriptional regulation. Methods like 'FOXP3 IHC' and 'FOXP3 IHC mouse' are useful for detecting this protein in tissue samples while 'FOXP3 antibodies' aid in research on this target. Techniques like 'FOXP3 staining' and 'FOXP3 flow cytometry' further allow for the analysis of its expression and function.
Biological function summary

This transcription factor operates by regulating the expression of a variety of genes involved in Treg cell development and function. FOXP3 can interact with other proteins to form complexes that modulate gene expression. Its role is essential in enabling Tregs to suppress immune responses a process necessary for preventing autoimmunity. The interaction extends to other key regulators within Tregs such as the protein complex 221D which may have overlapping functional responsibilities. 'FOXP3 236a/E7' reflects its genetic variants detectable in different studies providing insights into its biological functions.

Pathways

FOXP3 is a significant player within the immune regulation pathway. It integrates into the TGF-beta signaling pathway in which it controls the expression of genes important for Treg functions. The protein recruits other transcription factors like NF-kB coordinating intricate network regulations within immune cells. FOXP3's activity orchestrates the balance between immune activation and suppression ensuring a well-functioning immune response that prevents overreactions leading to autoimmune conditions.

FOXP3 mutations or dysregulation can link to autoimmune diseases like immune dysregulation polyendocrinopathy enteropathy X-linked syndrome (IPEX). This transcription factor's malfunction implicates cancer where inadequate Treg function can either suppress antitumor immunity or result in tumor progression. Proteins such as CTLA-4 which is also involved in immune regulation connect to FOXP3 in these contexts emphasizing FOXP3’s significant role in maintaining immune homeostasis and its impact on disease development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the FOXP3 gene is a transcriptional regulator critical for the development and inhibitory function of regulatory T-cells (Treg). It plays an essential role in immune system homeostasis by ensuring the suppressive function and stability of Treg cells and directly influencing conventional T-cells' expansion and function. FOXP3 can function as either a transcriptional repressor or activator, depending on its interactions with other factors, such as transcription factors, histone acetylases, and deacetylases. It co-activates genes like CTLA4 and TNFRSF18 and represses cytokine genes, including interleukin-2 (IL2) and interferon-gamma (IFNG), and inhibits cytokine production by repressing the activity of transcription factors RELA and NFATC2. FOXP3's association with histone acetylase KAT5 and deacetylase HDAC7 mediates IL2 repression, while its interaction with transcription factor RUNX1 modulates the expression of TNFRSF18, IL2RA, CTLA4, IL2, and IFNG. It also antagonizes RORC to inhibit the differentiation of IL17 producing helper T-cells (Th17), leading to reduced IL17 expression and promoting Treg development, while inhibiting the transcriptional activator activity of RORA. This supplementary information is collated from multiple sources and compiled automatically.
See full target information FOXP3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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