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Rabbit Recombinant Monoclonal FOXP4 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Mouse, Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-FOXP4 antibody [EPR22714-2] (AB307621), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-FOXP4 antibody [EPR22714-2] (AB307621), expandable thumbnail
  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-FOXP4 antibody [EPR22714-2] (AB307621), expandable thumbnail
  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-FOXP4 antibody [EPR22714-2] (AB307621), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)ICC/IF
Human
Tested
Tested
Mouse
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Species
Human
Dilution info
1/500
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Species
Human
Dilution info
1/50
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Transcriptional repressor that represses lung-specific expression.

Alternative names

FKHLA, FOXP4, Forkhead box protein P4, Fork head-related protein-like A

Rabbit Recombinant Monoclonal FOXP4 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Mouse, Human samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22714-2
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

FOXP4 also known as Forkhead Box P4 is a transcription factor belonging to the FOX protein family. Its molecular mass approximately 80 kDa. It is expressed in various tissues with high expression levels observed in lung kidney and prostate. The protein binds to DNA and regulates the transcription of genes involved in development and differentiation.

Biological function summary

FOXP4 plays an important role in regulating developmental processes particularly in the central nervous system and lung tissue. It often functions as part of a complex that includes other FOXP proteins like FOXP1 and FOXP2 which share a conserved DNA-binding forkhead domain. These proteins collaborate to regulate genes important for cell differentiation and organogenesis.

Pathways

The transcriptional activity of FOXP4 integrates into significant signaling pathways such as the Wnt signaling and TGF-beta pathways. These pathways govern processes like cellular growth and differentiation. FOXP4 interacts with other FOX proteins including FOXP1 to modulate gene expression patterns important for these pathways.

Associated diseases and disorders

Research indicates that FOXP4 is associated with lung cancer and neurodevelopmental disorders. Its dysregulation can affect cellular processes linked to tumorigenesis and cognitive development. FOXP4’s involvement with other proteins such as FOXP1 highlights its role in modulating gene networks that when disrupted can contribute to disease pathogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-FOXP4 antibody [EPR22714-2] (ab307621), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-FOXP4 antibody [EPR22714-2] (ab307621)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling FOXP4 with ab307621 at 1/50 (10.0 μg/ml) dilution. Confocal image showing nuclear staining in HepG2 cells. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5 μg/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-FOXP4 antibody [EPR22714-2] (ab307621), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-FOXP4 antibody [EPR22714-2] (ab307621)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell) cells labelling FOXP4 with ab307621 at 1/50 (10.0 μg/ml) dilution. Confocal image showing nuclear staining in Hepa1-6 cells. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5 μg/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-FOXP4 antibody [EPR22714-2] (ab307621), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-FOXP4 antibody [EPR22714-2] (ab307621)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling FOXP4 with ab307621 at 1/500 dilution (0.1μg) (Red) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-FOXP4 antibody [EPR22714-2] (ab307621), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-FOXP4 antibody [EPR22714-2] (ab307621)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Hepa1-6 (mouse hepatoma epithelial cell) cells labelling FOXP4 with ab307621 at 1/500 dilution (0.1μg) (Red) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com