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AB313591

Alexa Fluor® 647 Anti-GAD65 + GAD67 antibody [EPR19366]

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Rabbit Recombinant Monoclonal GAD67 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-Fr and reacts with Mouse, Rat samples.

View Alternative Names

Gad67, Gad1, Glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, Glutamate decarboxylase 67 kDa isoform, GAD-67

4 Images
Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GAD65 + GAD67 antibody [EPR19366] (AB313591)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GAD65 + GAD67 antibody [EPR19366] (AB313591)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney (fresh) tissue labeling GAD65 + GAD67 with ab313591 at 1/50 (10.0 ug/ml) dilution (Green). Low expression : confocal image showing no staining on mouse kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with ab313591 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GAD65 + GAD67 antibody [EPR19366] (AB313591)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GAD65 + GAD67 antibody [EPR19366] (AB313591)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney (fresh) tissue labeling GAD65 + GAD67 with ab313591 at 1/50 (10.0 ug/ml) dilution (Green). Low expression : confocal image showing no staining on rat kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with ab313591 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GAD65 + GAD67 antibody [EPR19366] (AB313591)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GAD65 + GAD67 antibody [EPR19366] (AB313591)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (fresh) tissue labeling GAD65 + GAD67 with ab313591 at 1/50 (10.0 ug/ml) dilution (Green). Confocal image showing positive staining on rat cerebellum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab313591 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GAD65 + GAD67 antibody [EPR19366] (AB313591)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GAD65 + GAD67 antibody [EPR19366] (AB313591)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh) tissue labeling GAD65 + GAD67 with ab313591 at 1/50 (10.0 ug/ml) dilution (Green). Confocal image showing positive staining on mouse cerebellum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab313591 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

  • Biotin

    Biotin Anti-GAD65 + GAD67 antibody [EPR19366]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-GAD65 + GAD67 antibody [EPR19366]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19366

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Mouse, Rat

Applications

IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "<p>Confocal image showing positive staining on mouse cerebellum. Low expression: confocal image showing no staining on mouse kidney. Confocal image showing positive staining on rat cerebellum. Low expression: confocal image showing no staining on rat kidney.</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "<p>Confocal image showing positive staining on mouse cerebellum. Low expression: confocal image showing no staining on mouse kidney. Confocal image showing positive staining on rat cerebellum. Low expression: confocal image showing no staining on rat kidney.</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) with pyridoxal 5'-phosphate as cofactor.
See full target information Gad1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com