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Anti-gamma H2A.X (phospho S139) [EP854(2)Y] Alexa Fluor® 647 conjugated antibody (ab195189) rabbit monoclonal antibody that is used to detect gamma H2A.X in IHC-P, ICC/IF. Suitable for Human samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency


Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB195189), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB195189), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB195189), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-P
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Sheep
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/200
Notes

-

Predicted
Predicted

Species
Mouse, Rat, Sheep
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat, Sheep
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

5 products for Alternative Version

Target data

Function

The protein expressed by the H2AX gene is a variant histone H2A that replaces conventional H2A in certain nucleosomes, which are responsible for wrapping and compacting DNA into chromatin. This compaction limits DNA accessibility to cellular machineries that require DNA as a template, placing histones at the center of transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is controlled through a complex array of post-translational histone modifications, known as the histone code, and nucleosome remodeling. The H2AX protein is essential for the checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for the efficient repair of DNA double strand breaks (DSBs), particularly when it undergoes C-terminal phosphorylation. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

H2AFX, H2AX, Histone H2AX, H2a/x, Histone H2A.X, H2AS139p, H2AXS139p, H2A.XS139p

Recommended products

Anti-gamma H2A.X (phospho S139) [EP854(2)Y] Alexa Fluor® 647 conjugated antibody (ab195189) rabbit monoclonal antibody that is used to detect gamma H2A.X in IHC-P, ICC/IF. Suitable for Human samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP854(2)Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

What is this antibody validated in?


Alexa Fluor® 647 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab195189) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in Human samples.

Recommended positive controls


ICC/IF: Jurkat cells treated with ETP.IHC-P: Human cerebrum tissue and human testis tissue.

Related products


Antibody clone EP854(2)Y is also available pre-conjugated to a variety of labels for your convenience – Anti-gamma H2A.X Alexa Fluor® 647 (phospho S139) [EP854(2)Y] (ab195189).

Other related products


We have a range of other formats of antibody clone [EP854(2)Y] also available for your convenience:
Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] ab81299, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] ab195188, Alexa Fluor® 647 - ab195189, HRP - HRP Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] ab195190, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] ab206898, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] ab206900, Carrier free - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] - BSA and Azide free ab215967



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Gamma H2A.X also known as phospho H2A.X or γH2A.X is a phosphorylated form of the histone variant H2A.X. It has a molecular weight of about 14 kilodaltons and occurs primarily in they nucleus. When DNA double-strand breaks (DSBs) occur serine 139 in H2A.X undergoes rapid phosphorylation resulting in gamma H2A.X. This modification happens swiftly at the site of damage and gamma H2A.X spreads over a large chromatin area facilitating the recruitment of DNA repair proteins. Gamma H2A.X staining typically evaluated using gamma H2A.X immunofluorescence techniques aids in identifying the presence and extent of DNA damage.

Biological function summary

Gamma H2A.X plays a role in DNA damage response and repair. It does not operate alone; it acts as part of a complex with other repair proteins. The formation of gamma H2A.X foci at DNA damage sites creates a signal attracting repair factors that help maintain genome stability. Its interaction with MDC1 and ATM proteins exemplifies its significant role in orchestrating an effective response to DNA damage. Beyond DNA repair gamma H2A.X influences cell cycle checkpoints permitting cells to pause and repair before proceeding with division.

Pathways

Gamma H2A.X plays a pivotal role in the DNA damage response (DDR) pathway. This pathway is essential for detecting and repairing DNA lesions to uphold genomic integrity. Within the DDR pathway gamma H2A.X is closely associated with proteins such as NBS1 and BRCA1 which assist in repairing double-strand breaks. In addition gamma H2A.X is integral to the ATM-ATR signaling pathway where its activation promotes cell survival following genotoxic stress by signaling for damage repair or triggering apoptosis.

Associated diseases and disorders

Gamma H2A.X has connections to cancer and neurodegeneration. Aberrant DNA repair pathways often indicated by persistent gamma H2A.X signals correlate with tumor formation and progression. For instance a failure to repair DNA damage effectively can lead to mutations that drive cancer development. Gamma H2A.X also links to neurodegenerative diseases where dysregulated DNA repair contributes to neuronal cell death. Proteins like p53 which regulate cell cycle and apoptosis further connect to gamma H2A.X bridging its role in disease pathogenesis.

Product promise

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3 product images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab195189), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab195189)

    ab195189 staining Histone H2A.X in Jurkat cells. The cells were incubated with 25uM ETP for 5 hours (Treated) or solvent-only for control purposes (Non-treated). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 10% normal goat serum in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab195189 at 1/200 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/200 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab195189), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab195189)

    Immunohistochemistry analysis of paraffin-embedded human testis tissue sections labelling gamma H2A.X (phospho S139) with ab195189 at 1/100 dilution. The section was incubated with ab195189 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins.
    Nuclear staining on human testis. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
    Negative control : PBS was used as a negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab195189), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab195189)

    Immunohistochemistry analysis of paraffin-embedded human cerebrum tissue sections labelling gamma H2A.X (phospho S139) with ab195189 at 1/100 dilution. The section was incubated with ab195189 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins.
    Nuclear staining on human cerebrum. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
    Negative control : PBS was used as a negative control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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