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AB195189

Alexa Fluor® 647 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

2

(2 Reviews)

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(11 Publications)

Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] - Alexa Fluor® 647 conjugated (ab195189) is a rabbit recombinant monoclonal antibody detecting gamma H2A.X in IHC-P, ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

H2AFX, H2AX, Histone H2AX, H2a/x, Histone H2A.X, H2AS139p, H2AXS139p, H2A.XS139p

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB195189)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB195189)

Immunohistochemistry analysis of paraffin-embedded human cerebrum tissue sections labelling gamma H2A.X (phospho S139) with ab195189 at 1/100 dilution. The section was incubated with ab195189 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins. Nuclear staining on human cerebrum. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Negative control : PBS was used as a negative control.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB195189)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB195189)

ab195189 staining Histone H2A.X in Jurkat cells. The cells were incubated with 25uM ETP for 5 hours (Treated) or solvent-only for control purposes (Non-treated). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 10% normal goat serum in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab195189 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/200 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB195189)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB195189)

Immunohistochemistry analysis of paraffin-embedded human testis tissue sections labelling gamma H2A.X (phospho S139) with ab195189 at 1/100 dilution. The section was incubated with ab195189 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins. Nuclear staining on human testis. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Negative control : PBS was used as a negative control.

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

  • HRP

    HRP Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

  • Unconjugated

    Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

  • Carrier free

    Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP854(2)Y

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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AB22551

Anti-gamma H2A.X (phospho S139) antibody [3F2]

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Primary Antibodies

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Primary Antibodies

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Anti-gamma H2A.X (phospho S139) antibody - C-terminal

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Primary Antibodies

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Primary Antibodies

AB2893

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Complementary Products

Assay Kits

AB279816

Phospho-gamma H2A.X (S139) ELISA Kit

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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Anti-Rad51 antibody [EPR4030(3)]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [EPR4030(3)] (AB133534)

  • 4
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Primary Antibodies

AB32042

Anti-Cleaved Caspase-3 antibody [E83-77]

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  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

What is this antibody validated in?
Alexa Fluor® 647 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab195189) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Related products
Antibody clone EP854(2)Y is also available pre-conjugated to a variety of labels for your convenience – Anti-gamma H2A.X Alexa Fluor® 647 (phospho S139) [EP854(2)Y] (ab195189).

Other related products
We have a range of other formats of antibody clone [EP854(2)Y] also available for your convenience: ab81299, Alexa Fluor® 488 - ab195188, Alexa Fluor® 647 - ab195189, HRP - ab195190, Alexa Fluor® 594 - ab206898, Alexa Fluor® 555 - ab206900, Carrier free - ab215967

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gamma H2A.X also known as phospho H2A.X or γH2A.X is a phosphorylated form of the histone variant H2A.X. It has a molecular weight of about 14 kilodaltons and occurs primarily in they nucleus. When DNA double-strand breaks (DSBs) occur serine 139 in H2A.X undergoes rapid phosphorylation resulting in gamma H2A.X. This modification happens swiftly at the site of damage and gamma H2A.X spreads over a large chromatin area facilitating the recruitment of DNA repair proteins. Gamma H2A.X staining typically evaluated using gamma H2A.X immunofluorescence techniques aids in identifying the presence and extent of DNA damage.
Biological function summary

Gamma H2A.X plays a role in DNA damage response and repair. It does not operate alone; it acts as part of a complex with other repair proteins. The formation of gamma H2A.X foci at DNA damage sites creates a signal attracting repair factors that help maintain genome stability. Its interaction with MDC1 and ATM proteins exemplifies its significant role in orchestrating an effective response to DNA damage. Beyond DNA repair gamma H2A.X influences cell cycle checkpoints permitting cells to pause and repair before proceeding with division.

Pathways

Gamma H2A.X plays a pivotal role in the DNA damage response (DDR) pathway. This pathway is essential for detecting and repairing DNA lesions to uphold genomic integrity. Within the DDR pathway gamma H2A.X is closely associated with proteins such as NBS1 and BRCA1 which assist in repairing double-strand breaks. In addition gamma H2A.X is integral to the ATM-ATR signaling pathway where its activation promotes cell survival following genotoxic stress by signaling for damage repair or triggering apoptosis.

Gamma H2A.X has connections to cancer and neurodegeneration. Aberrant DNA repair pathways often indicated by persistent gamma H2A.X signals correlate with tumor formation and progression. For instance a failure to repair DNA damage effectively can lead to mutations that drive cancer development. Gamma H2A.X also links to neurodegenerative diseases where dysregulated DNA repair contributes to neuronal cell death. Proteins like p53 which regulate cell cycle and apoptosis further connect to gamma H2A.X bridging its role in disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
See full target information H2AX phospho S139
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

European journal of histochemistry : EJH 68: PubMed39526436

2024

Activation of Hedgehog pathway by circEEF2/miR-625-5p/TRPM2 axis promotes prostate cancer cell proliferation through mitochondrial stress.

Applications

Unspecified application

Species

Unspecified reactive species

ChenHui Zhu,LiJuan Lin,ChangQing Huang,ZhiHui Wu

Bioactive materials 42:316-327 PubMed39290339

2024

3D bioprinted breast cancer model reveals stroma-mediated modulation of extracellular matrix and radiosensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Theo Desigaux,Leo Comperat,Nathalie Dusserre,Marie-Laure Stachowicz,Malou Lea,Jean-William Dupuy,Anthony Vial,Michael Molinari,Jean-Christophe Fricain,François Paris,Hugo Oliveira

Heliyon 10:e28488 PubMed38590861

2024

Hyperthermia improves gemcitabine sensitivity of pancreatic cancer cells by suppressing the EFNA4/β-catenin axis and activating dCK.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaoxian He,Yangyang Zheng,Lei Lu,Hongzhang Shen,Weigang Gu,Jianfeng Yang,Xiaofeng Zhang,Hangbin Jin

Frontiers in cell and developmental biology 10:1070599 PubMed36568985

2022

G2/M checkpoint regulation and apoptosis facilitate the nuclear egress of parvoviral capsids.

Applications

Unspecified application

Species

Unspecified reactive species

Salla Mattola,Elina Mäntylä,Vesa Aho,Sami Salminen,Simon Leclerc,Mikko Oittinen,Kari Salokas,Jani Järvensivu,Satu Hakanen,Teemu O Ihalainen,Keijo Viiri,Maija Vihinen-Ranta

Biomedicines 10: PubMed36140359

2022

β-Caryophyllene Counteracts Chemoresistance Induced by Cigarette Smoke in Triple-Negative Breast Cancer MDA-MB-468 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Antonella Di Sotto,Marco Gullì,Marco Minacori,Romina Mancinelli,Stefania Garzoli,Ester Percaccio,Alessio Incocciati,Donatella Romaniello,Gabriela Mazzanti,Margherita Eufemi,Silvia Di Giacomo

PLoS pathogens 18:e1010353 PubMed35395063

2022

Parvovirus nonstructural protein 2 interacts with chromatin-regulating cellular proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Salla Mattola,Kari Salokas,Vesa Aho,Elina Mäntylä,Sami Salminen,Satu Hakanen,Einari A Niskanen,Julija Svirskaite,Teemu O Ihalainen,Kari J Airenne,Minna Kaikkonen-Määttä,Colin R Parrish,Markku Varjosalo,Maija Vihinen-Ranta

Bioengineered 13:5685-5699 PubMed34696659

2021

MicroRNA-326 impairs chemotherapy resistance in non small cell lung cancer by suppressing histone deacetylase SIRT1-mediated HIF1α and elevating VEGFA.

Applications

Unspecified application

Species

Unspecified reactive species

Jinying Wei,Guangping Meng,Jing Wu,Ying Wang,Qiang Zhang,Ting Dong,Jin Bao,Chunyan Wang,Jie Zhang

Cell reports methods 1: PubMed34485971

2021

Toward reproducible, scalable, and robust data analysis across multiplex tissue imaging platforms.

Applications

Unspecified application

Species

Unspecified reactive species

Erik A Burlingame,Jennifer Eng,Guillaume Thibault,Koei Chin,Joe W Gray,Young Hwan Chang

American journal of translational research 13:5835-5850 PubMed34306329

2021

Long non-coding RNA H19 and the underlying epigenetic function in response to DNA damage of lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dongjie Wang,Yajiao Sun,Lin Lin,Yulan Sang,Fan Yang,Jiawen Zhang,Li Jia,Ziping Xu,Wei Zhang

Medical science monitor : international medical jo 24:1424-1433 PubMed29519997

2018

Overexpression of Nitrogen Permease Regulator Like-2 (NPRL2) Enhances Sensitivity to Irinotecan (CPT-11) in Colon Cancer Cells by Activating the DNA Damage Checkpoint Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shasha Liu,Bingrong Liu
View all publications
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Product promise

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