Mouse Monoclonal TBG1 antibody - conjugated to Alexa Fluor® 647. C-terminal. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human Tubulin gamma-1 chain aa 400-450 conjugated to Keyhole Limpet Haemocyanin.
Images
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-gamma Tubulin antibody [TU-30] - C-terminal (AB191114), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-gamma-tubulin-antibody-tu-30-c-terminal-ab191114--immunocytochemistry-immunofluorescence-img103365.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-gamma Tubulin antibody [TU-30] - C-terminal (AB191114)")
![Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-gamma Tubulin antibody [TU-30] - C-terminal (AB191114), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-gamma-tubulin-antibody-tu-30-c-terminal-ab191114--flow-cytometry-intracellular-img418752.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-gamma Tubulin antibody [TU-30] - C-terminal (AB191114)")
Publications
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- eLife 11:2022GJA1 depletion causes ciliary defects by affecting Rab11 trafficking to the ciliary base.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Gil Jang,Keun Yeong Kwon,Yeong Cheon Kweon,Byung-Gyu Kim,Kyungjae Myung,Hyun-Shik Lee,Chan Young Park,Taejoon Kwon,Tae Joo ParkPubMed 36004726
- Communications biology 4:13712021Centrosome dysfunction associated with somatic expression of the synaptonemal complex protein TEX12.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSumit Sandhu,Ieng F Sou,Jill E Hunter,Lucy Salmon,Caroline L Wilson,Neil D Perkins,Neil Hunter,Owen R Davies,Urszula L McClurgPubMed 34880391
- Nature communications 12:6462021Proliferative polyploid cells give rise to tumors via ploidy reduction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomonori Matsumoto,Leslie Wakefield,Alexander Peters,Myron Peto,Paul Spellman,Markus GrompePubMed 33510149
- Scientific data 6:3232019Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer.
- Clinical cancer research : an official journal of 25:4179-41932019Unexpected Activities in Regulating Ciliation Contribute to Off-target Effects of Targeted Drugs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna A Kiseleva et. al.PubMed 30867219
Key facts
- Isotype
IgG1
- Host species
Mouse
- Conjugation
Alexa Fluor® 647
- Excitation/Emission
Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: 99% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Synthetic Peptide within Human Tubulin gamma-1 chain aa 400-450 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P23258
Reactivity data
Select an application| ICC/IF | Flow Cyt (Intra) | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-5.00000 µg/mL | Notes Intracellular staining. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.
Alternative names
TUBG, TUBG, TUBG1, Tubulin gamma-1 chain, Gamma-1-tubulin, Gamma-tubulin complex component 1, GCP-1
Recommended products
Mouse Monoclonal TBG1 antibody - conjugated to Alexa Fluor® 647. C-terminal. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human Tubulin gamma-1 chain aa 400-450 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Conjugation
Alexa Fluor® 647
- Excitation/Emission
Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: 99% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Synthetic Peptide within Human Tubulin gamma-1 chain aa 400-450 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P23258
- Clone number
TU-30
- Purification technique
Size-exclusion chromatography
- Specificity
Immunogen has 94% identity with TUBG2 (Uniprot: Q9NRH3).
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Store in the dark
Notes
Also reacts with Protozoa.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Gamma-tubulin an important component in the microtubule nucleation process is also known as Gtub or γ-tub. It weighs approximately 48 kDa. Gamma-tubulin is a significant protein inside the centrosome where it plays a role in the formation of microtubule organizing centers. Scientists often find and express it in abundance within rapidly dividing cells both in humans and other eukaryotes. Researchers employ gamma-tubulin staining techniques to visualize its location and understand its function within the centrosome.
- Biological function summary
Gamma-tubulin is part of the gamma-tubulin ring complex also known as gamma-TuRC which anchors the microtubule minus ends influencing microtubule stability and organization. This complex functions as a nucleating template for microtubules which are critical for various cell processes including intracellular transport and chromosome separation during cell division. Gamma-tubulin plays an indispensable role in maintaining the correct function of the centrosome an important cellular organelle in cell cycle progression.
- Pathways
Gamma-tubulin participates in processes involving cell cycle regulation and mitotic spindle formation. These processes depend heavily on microtubule dynamics and organization. Gamma-tubulin interacts closely with proteins like centrins and pericentrin within these pathways. Such interactions ensure accurate chromosomal segregation during mitosis highlighting its importance in cellular reproduction and stability.
- Associated diseases and disorders
Abnormal gamma-tubulin expression and function relate to conditions like cancer and neurological disorders. Changes in gamma-tubulin levels can affect the centrosome's function and microtubule dynamics leading to cell cycle dysregulation and tumorigenesis. Moreover it interacts with mutated tumor suppressor proteins like p53 further contributing to cancer progression. Scientists continue to study gamma-tubulin's role in these diseases providing insights into new therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-gamma Tubulin antibody [TU-30] - C-terminal (ab191114), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-gamma-tubulin-antibody-tu-30-c-terminal-ab191114--immunocytochemistry-immunofluorescence-img103365.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-gamma Tubulin antibody [TU-30] - C-terminal (ab191114)")
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-gamma Tubulin antibody [TU-30] - C-terminal (ab191114)
Immunocytochemistry/ Immunofluorescence analysis of P19X1 mouse embryonal carcinoma cell line labeling gamma Tubulin with ab191114 at 1 μg/mL.
Nuclei were stained with DAPI (blue).
![Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-gamma Tubulin antibody [TU-30] - C-terminal (ab191114), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-gamma-tubulin-antibody-tu-30-c-terminal-ab191114--flow-cytometry-intracellular-img418752.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-gamma Tubulin antibody [TU-30] - C-terminal (ab191114)")
Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-gamma Tubulin antibody [TU-30] - C-terminal (ab191114)
Intracellular flow cytometric analysis of HeLa cells labeling gamma Tubulin with ab191114 used at 5μg/ml (red-filled) compared with a mouse IgG1 isotype control (MOPC-21) Alexa Fluor® 647 antibody (Alexa Fluor® 647 Mouse IgG1 kappa [MOPC-21] - Isotype Control ab239459) at 5μg/ml (black-dashed).
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