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AB191114

Alexa Fluor® 647 Anti-gamma Tubulin antibody [TU-30] - C-terminal - Centrosome Marker

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(5 Publications)

Mouse Monoclonal TBG1 antibody - conjugated to Alexa Fluor® 647. C-terminal. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human TUBG1 aa 400-450 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

TUBG, TUBG1, Tubulin gamma-1 chain, Gamma-1-tubulin, Gamma-tubulin complex component 1, GCP-1

2 Images
Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-gamma Tubulin antibody [TU-30] - C-terminal - Centrosome Marker (AB191114)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-gamma Tubulin antibody [TU-30] - C-terminal - Centrosome Marker (AB191114)

Intracellular flow cytometric analysis of HeLa cells labeling gamma Tubulin with ab191114 used at 5μg/ml (red-filled) compared with a mouse IgG1 isotype control (MOPC-21) Alexa Fluor® 647 antibody (ab239459) at 5μg/ml (black-dashed).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-gamma Tubulin antibody [TU-30] - C-terminal - Centrosome Marker (AB191114)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-gamma Tubulin antibody [TU-30] - C-terminal - Centrosome Marker (AB191114)

Immunocytochemistry/ Immunofluorescence analysis of P19X1 mouse embryonal carcinoma cell line labeling gamma Tubulin with ab191114 at 1 μg/mL.

Nuclei were stained with DAPI (blue).

  • Unconjugated

    Anti-gamma Tubulin antibody [TU-30] - Centrosome Marker

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

TU-30

Isotype

IgG1

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human TUBG1 aa 400-450 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P23258

Specificity

Immunogen has 94% identity with TUBG2 (Uniprot: Q9NRH3).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1-5 µg/mL", "FlowCytIntra-species-notes": "<p>Intracellular staining.</p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Also reacts with Protozoa.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: 99% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gamma-tubulin an important component in the microtubule nucleation process is also known as Gtub or γ-tub. It weighs approximately 48 kDa. Gamma-tubulin is a significant protein inside the centrosome where it plays a role in the formation of microtubule organizing centers. Scientists often find and express it in abundance within rapidly dividing cells both in humans and other eukaryotes. Researchers employ gamma-tubulin staining techniques to visualize its location and understand its function within the centrosome.
Biological function summary

Gamma-tubulin is part of the gamma-tubulin ring complex also known as gamma-TuRC which anchors the microtubule minus ends influencing microtubule stability and organization. This complex functions as a nucleating template for microtubules which are critical for various cell processes including intracellular transport and chromosome separation during cell division. Gamma-tubulin plays an indispensable role in maintaining the correct function of the centrosome an important cellular organelle in cell cycle progression.

Pathways

Gamma-tubulin participates in processes involving cell cycle regulation and mitotic spindle formation. These processes depend heavily on microtubule dynamics and organization. Gamma-tubulin interacts closely with proteins like centrins and pericentrin within these pathways. Such interactions ensure accurate chromosomal segregation during mitosis highlighting its importance in cellular reproduction and stability.

Abnormal gamma-tubulin expression and function relate to conditions like cancer and neurological disorders. Changes in gamma-tubulin levels can affect the centrosome's function and microtubule dynamics leading to cell cycle dysregulation and tumorigenesis. Moreover it interacts with mutated tumor suppressor proteins like p53 further contributing to cancer progression. Scientists continue to study gamma-tubulin's role in these diseases providing insights into new therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.
See full target information TUBG1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

eLife 11: PubMed36004726

2022

GJA1 depletion causes ciliary defects by affecting Rab11 trafficking to the ciliary base.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Gil Jang,Keun Yeong Kwon,Yeong Cheon Kweon,Byung-Gyu Kim,Kyungjae Myung,Hyun-Shik Lee,Chan Young Park,Taejoon Kwon,Tae Joo Park

Communications biology 4:1371 PubMed34880391

2021

Centrosome dysfunction associated with somatic expression of the synaptonemal complex protein TEX12.

Applications

Unspecified application

Species

Unspecified reactive species

Sumit Sandhu,Ieng F Sou,Jill E Hunter,Lucy Salmon,Caroline L Wilson,Neil D Perkins,Neil Hunter,Owen R Davies,Urszula L McClurg

Nature communications 12:646 PubMed33510149

2021

Proliferative polyploid cells give rise to tumors via ploidy reduction.

Applications

Unspecified application

Species

Unspecified reactive species

Tomonori Matsumoto,Leslie Wakefield,Alexander Peters,Myron Peto,Paul Spellman,Markus Grompe

Scientific data 6:323 PubMed31848351

2019

Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer.

Applications

mIHC

Species

Human

Rumana Rashid,Giorgio Gaglia,Yu-An Chen,Jia-Ren Lin,Ziming Du,Zoltan Maliga,Denis Schapiro,Clarence Yapp,Jeremy Muhlich,Artem Sokolov,Peter Sorger,Sandro Santagata

Clinical cancer research : an official journal of 25:4179-4193 PubMed30867219

2019

Unexpected Activities in Regulating Ciliation Contribute to Off-target Effects of Targeted Drugs.

Applications

Unspecified application

Species

Unspecified reactive species

Anna A Kiseleva,Vladislav A Korobeynikov,Anna S Nikonova,Peishan Zhang,Petr Makhov,Alexander Y Deneka,Margret B Einarson,Ilya G Serebriiskii,Hanqing Liu,Jeffrey R Peterson,Erica A Golemis
View all publications
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Product promise

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