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AB215227

Alexa Fluor® 647 Anti-GAPDH antibody [EPR6256]

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(11 Publications)

Rabbit Recombinant Monoclonal GAPDH antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 11 publications.

View Alternative Names

GAPD, CDABP0047, OK/SW-cl.12, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GAPDH antibody [EPR6256] (AB215227)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GAPDH antibody [EPR6256] (AB215227)

ab215227 staining GAPDH in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab215227 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Carrier free

    Anti-GAPDH antibody [EPR6256] - BSA and Azide free

  • Unconjugated

    Anti-GAPDH antibody [EPR6256] - Loading Control

  • 660 APC

    APC Anti-GAPDH antibody [EPR6256]

  • Biotin

    Biotin Anti-GAPDH antibody [EPR6256] - Loading Control

  • HRP

    HRP Anti-GAPDH antibody [EPR6256] - Loading Control

  • 675 PerCP

    PerCP Anti-GAPDH antibody [EPR6256]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6256

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Human

Applications

Flow Cyt (Intra), ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (AB201822)

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

AB52866

Anti-alpha Tubulin antibody [EP1332Y] - Loading Control

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "African green monkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.
Biological function summary

GAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.

Pathways

GAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.

GAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis (PubMed : 11724794, PubMed : 3170585). Also shows nitrosylase activity, thereby playing a role in nuclear functions (PubMed : 11724794, PubMed : 3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed : 23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed : 23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed : 23332158, PubMed : 27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).
See full target information GAPDH
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Methods & clinical development 32:101234 PubMed38558569

2024

Novel AAV variants with improved tropism for human Schwann cells.

Applications

Unspecified application

Species

Unspecified reactive species

Matthieu Drouyer,Tak-Ho Chu,Elodie Labit,Florencia Haase,Renina Gale Navarro,Deborah Nazareth,Nicole Rosin,Jessica Merjane,Suzanne Scott,Marti Cabanes-Creus,Adrian Westhaus,Erhua Zhu,Rajiv Midha,Ian E Alexander,Jeff Biernaskie,Samantha L Ginn,Leszek Lisowski

Molecular therapy : the journal of the American Society of Gene Therapy 32:818-836 PubMed38297833

2024

Development of CNS tropic AAV1-like variants with reduced liver-targeting following systemic administration in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Matthieu Drouyer,Jessica Merjane,Deborah Nazareth,Maddison Knight,Suzanne Scott,Sophia H Y Liao,Samantha L Ginn,Erhua Zhu,Ian E Alexander,Leszek Lisowski

Human gene therapy 34:917-926 PubMed37350098

2023

Recapitulation of Skewed X-Inactivation in Female Ornithine Transcarbamylase-Deficient Primary Human Hepatocytes in the FRG Mouse: A Novel System for Developing Epigenetic Therapies.

Applications

Unspecified application

Species

Unspecified reactive species

Sharon C Cunningham,Eva B van Dijk,Erhua Zhu,Maya Sugden,Mawj Mandwie,Susan Siew,Beena Devanapalli,Adviye Ayper Tolun,Anne Klein,Laurence Wilson,Nader Aryamanesh,Paul Gissen,Julien Baruteau,Kaustuv Bhattacharya,Ian E Alexander

Molecular therapy. Methods & clinical development 28:220-237 PubMed36700121

2023

Characterization of the humanized FRG mouse model and development of an AAV-LK03 variant with improved liver lobular biodistribution.

Applications

Unspecified application

Species

Unspecified reactive species

Marti Cabanes-Creus,Renina Gale Navarro,Sophia H Y Liao,Suzanne Scott,Rodrigo Carlessi,Ramon Roca-Pinilla,Maddison Knight,Grober Baltazar,Erhua Zhu,Matthew Jones,Elena Denisenko,Alistair R R Forrest,Ian E Alexander,Janina E E Tirnitz-Parker,Leszek Lisowski

Human gene therapy 33:664-682 PubMed35297686

2022

AAV-p40 Bioengineering Platform for Variant Selection Based on Transgene Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Adrian Westhaus,Marti Cabanes-Creus,Timo Jonker,Erwan Sallard,Renina Gale Navarro,Erhua Zhu,Grober Baltazar Torres,Scott Lee,Patrick Wilmott,Anai Gonzalez-Cordero,Giorgia Santilli,Adrian J Thrasher,Ian E Alexander,Leszek Lisowski

Micromachines 13: PubMed35056257

2022

A Cell Culture Chip with Transparent, Micropillar-Decorated Bottom for Live Cell Imaging and Screening of Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Menekse Ermis,Ezgi Antmen,Ozgur Kuren,Utkan Demirci,Vasif Hasirci

Molecular therapy. Methods & clinical development 24:88-101 PubMed34977275

2022

Novel human liver-tropic AAV variants define transferable domains that markedly enhance the human tropism of AAV7 and AAV8.

Applications

Unspecified application

Species

Unspecified reactive species

Marti Cabanes-Creus,Renina Gale Navarro,Erhua Zhu,Grober Baltazar,Sophia H Y Liao,Matthieu Drouyer,Anais K Amaya,Suzanne Scott,Loan Hanh Nguyen,Adrian Westhaus,Matthias Hebben,Laurence O W Wilson,Adrian J Thrasher,Ian E Alexander,Leszek Lisowski

Molecular therapy. Methods & clinical development 21:607-620 PubMed34095344

2021

Single amino acid insertion allows functional transduction of murine hepatocytes with human liver tropic AAV capsids.

Applications

Unspecified application

Species

Unspecified reactive species

Marti Cabanes-Creus,Renina Gale Navarro,Sophia H Y Liao,Grober Baltazar,Matthieu Drouyer,Erhua Zhu,Suzanne Scott,Clement Luong,Laurence O W Wilson,Ian E Alexander,Leszek Lisowski

Science translational medicine 12: PubMed32908003

2020

Restoring the natural tropism of AAV2 vectors for human liver.

Applications

Unspecified application

Species

Unspecified reactive species

Marti Cabanes-Creus,Claus V Hallwirth,Adrian Westhaus,Boaz H Ng,Sophia H Y Liao,Erhua Zhu,Renina Gale Navarro,Grober Baltazar,Matthieu Drouyer,Suzanne Scott,Grant J Logan,Giorgia Santilli,Antonette Bennett,Samantha L Ginn,Geoff McCaughan,Adrian J Thrasher,Mavis Agbandje-McKenna,Ian E Alexander,Leszek Lisowski

Molecular therapy. Methods & clinical development 17:1139-1154 PubMed32490035

2020

Attenuation of Heparan Sulfate Proteoglycan Binding Enhances Transduction of Human Primary Hepatocytes with AAV2.

Applications

Unspecified application

Species

Unspecified reactive species

Marti Cabanes-Creus,Adrian Westhaus,Renina Gale Navarro,Grober Baltazar,Erhua Zhu,Anais K Amaya,Sophia H Y Liao,Suzanne Scott,Erwan Sallard,Kimberley L Dilworth,Arkadiusz Rybicki,Matthieu Drouyer,Claus V Hallwirth,Antonette Bennett,Giorgia Santilli,Adrian J Thrasher,Mavis Agbandje-McKenna,Ian E Alexander,Leszek Lisowski
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Product promise

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