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Rabbit Recombinant Monoclonal GATAD2B antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GATAD2B antibody [EPR27329-3] (AB317760), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GATAD2B antibody [EPR27329-3] (AB317760), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GATAD2B antibody [EPR27329-3] (AB317760), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GATAD2B antibody [EPR27329-3] (AB317760), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Human
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Transcriptional repressor (PubMed:12183469, PubMed:16415179). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:28977666). Enhances MBD2-mediated repression (PubMed:12183469, PubMed:16415179). Efficient repression requires the presence of GATAD2A (PubMed:16415179). Targets MBD3 to discrete loci in the nucleus (PubMed:11756549). May play a role in synapse development (PubMed:23644463).

Alternative names

KIAA1150, GATAD2B, Transcriptional repressor p66-beta, GATA zinc finger domain-containing protein 2B, p66/p68

Recommended products

Rabbit Recombinant Monoclonal GATAD2B antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27329-3
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

GATAD2B also known as p66β is a protein which forms part of the nucleosome remodeling and deacetylase (NuRD) complex. The protein has a mass of approximately 66 kDa. It is expressed in various human tissues including brain and skeletal muscle. GATAD2B plays a vital role in transcriptional repression by recruiting chromatin remodeling factors which influence gene expression regulation.

Biological function summary

GATAD2B influences chromatin structure and gene expression. It functions as an integral component of the NuRD complex which combines ATP-dependent chromatin remodeling with histone deacetylase activity. The interaction of GATAD2B with other NuRD components such as MBD2 and CHD3 demonstrates its role in modifying the accessibility of DNA to transcription factors impacting cellular activities like differentiation.

Pathways

Other components of the NuRD complex work together with GATAD2B in the regulation of the Wnt signaling pathway and the Notch signaling pathway. These pathways are important for cellular proliferation and differentiation. GATAD2B has functional connections with other proteins including REST and HDAC1 and these connections indicate its involvement in these pathways that shape developmental processes and cellular responses.

Associated diseases and disorders

GATAD2B is significant in connection with neurodevelopmental disorders. Pathogenic mutations in the GATAD2B gene have been linked to GATAD2B-associated neurodevelopmental disorder. It is also associated with intellectual disability syndromes. GATAD2B shows interactions with proteins such as TCF4 and SMARCA2 which are also linked to similar neurodevelopmental conditions illustrating its impact on brain function and development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GATAD2B antibody [EPR27329-3] (ab317760), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GATAD2B antibody [EPR27329-3] (ab317760)

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling GATAD2B with ab317760 at 1/50 (10.0 ug/ml) dilution.



    Nuclear staining on rat colon.The section was incubated with ab317760 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GATAD2B antibody [EPR27329-3] (ab317760), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GATAD2B antibody [EPR27329-3] (ab317760)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling GATAD2B with ab317760 at 1/50 (10.0 ug/ml) dilution.



    Nuclear staining on mouse colon.The section was incubated with ab317760 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GATAD2B antibody [EPR27329-3] (ab317760), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GATAD2B antibody [EPR27329-3] (ab317760)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling GATAD2B with ab317760 at 1/50 (10.0 ug/ml) dilution.



    Nuclear staining on human colon.The section was incubated with ab317760 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GATAD2B antibody [EPR27329-3] (ab317760), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GATAD2B antibody [EPR27329-3] (ab317760)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling GATAD2B with ab317760 at 1/50 (10.0 ug/ml) dilution.



    Nuclear staining on human tonsil.The section was incubated with ab317760 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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