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Rabbit Recombinant Monoclonal GFAP antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Mouse, Rat samples.


Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFAP antibody [EP672Y] - Astrocyte Marker (AB302828), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFAP antibody [EP672Y] - Astrocyte Marker (AB302828), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Species
Rat
Dilution info
1/50
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Version

Target data

Function

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Alternative names

Glial fibrillary acidic protein, GFAP, Gfap

Recommended products

Rabbit Recombinant Monoclonal GFAP antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP672Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFAP antibody [EP672Y] - Astrocyte Marker (ab302828), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFAP antibody [EP672Y] - Astrocyte Marker (ab302828)

    GFAP Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-GFAP antibody

    Immunocytochemical analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat primary neural/glia cells staining GFAP using ab302828 at a 1/50 dilution (10.0 μg/ml) (Red). Counterstained with Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody at a 1/100 dilution (1 μg/ml) followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at a 1/1000 dilution (2 μg/ml) (Green). Nuclear counterstain is DAPI (Blue).
    Confocal image showing positive staining in rat primary glia cell. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFAP antibody [EP672Y] - Astrocyte Marker (ab302828), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFAP antibody [EP672Y] - Astrocyte Marker (ab302828)

    GFAP Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-GFAP antibody

    Immunocytochemical analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells staining GFAP using ab302828 at a 1/50 dilution (10.0 μg/ml) (Red). Counterstained with Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody at a 1/100 dilution (1 μg/ml) followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at a 1/1000 dilution (2 μg/ml) (Green). Nuclear counterstain is DAPI (Blue).
    Confocal image showing positive staining in mouse primary glia cell. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com