AB194325

Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

Name: Anti-GFAP Alexa Fluor® 647 antibody [EPR1034Y] (ab194325) IgG | Abcam
Brand: Abcam Limited
SKU: AB194325
Price: 620 USD
Availability: InStock
Rating: 3 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(5 Publications )

Rabbit Recombinant Monoclonal GFAP antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-Fr, Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 5 publications.

View Alternative Names

Glial fibrillary acidic protein, GFAP

1 Images

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB194325)

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB194325)

IHC image of ab194325 staining in 10% formaldehyde fixed frozen tissue section of normal rat brain.

Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with ab194325 (1/100 dilution) and DAPI in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C.

The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor^® 647 signal is derived directly from bound ab194325.

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.

Unconjugated

Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker
Mouse version

Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 (Chimeric) - BSA and Azide free
Mouse version

Anti-GFAP antibody [EPR1034Y] - Mouse IgG2a (Chimeric) - BSA and Azide free
Rat version

Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) - BSA and Azide free
Mouse version

Anti-GFAP antibody [EPR1034Y] - Astrocyte marker - Mouse IgG1 (Chimeric)
Mouse version

Anti-GFAP antibody [EPR1034Y] - Astrocyte marker - Mouse IgG2a (Chimeric)
Rat version

Anti-GFAP antibody [EPR1034Y] - Astrocyte marker - Rat IgG2a (Chimeric)
Chicken version

Anti-GFAP antibody [EPR1034Y] - Astrocyte marker - Chicken IgY (Chimeric)
Chicken version

Anti-GFAP antibody [EPR1034Y] – Chicken IgY (Chimeric) – BSA and Azide Free
519 Mouse version

Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 (Chimeric)
519 Rat version

Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric)
519 Mouse version

Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] - Mouse IgG2a (Chimeric)
665 Mouse version

Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric
665 Mouse version

Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG2a (Chimeric)
665 Rat version

Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric)
Carrier free

Anti-GFAP antibody [EPR1034Y] - BSA and Azide free
660 APC

APC Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker
421 Alexa Fluor® 405

Alexa Fluor® 405 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker
Biotin

Biotin Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker
HRP

HRP Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker
578 PE

PE Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1034Y

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Rat

Applications

ICC/IF, IHC-Fr, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/500", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

See full target information GFAP

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113334 PubMed40917877

2025

Human cerebral organoids model tumor initiation and infiltration in an autologous astrocyte-supported setting.

Applications

Unspecified application

Species

Unspecified reactive species

Esther Schickel,Tamara Bender,Leon Kaysan,Simone Hufgard,Margot Mayer,David R Grosshans,Christiane Thielemann,Insa S Schroeder

Frontiers in pharmacology 15:1428924 PubMed39135794

2024

Soloxolone -methylanilide effectively suppresses aggressive phenotype of glioblastoma cells including TGF-β1-induced glial-mesenchymal transition and inhibits growth of U87 glioblastoma xenografts in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kirill V Odarenko,Aleksandra V Sen'kova,Oksana V Salomatina,Oleg V Markov,Nariman F Salakhutdinov,Marina A Zenkova,Andrey V Markov

Nature metabolism 5:1820-1835 PubMed37798473

2023

Spatially resolved metabolomics and isotope tracing reveal dynamic metabolic responses of dentate granule neurons with acute stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Miller,Elisa M York,Sylwia A Stopka,Juan Ramón Martínez-François,Md Amin Hossain,Gerard Baquer,Michael S Regan,Nathalie Y R Agar,Gary Yellen

Cell 185:2213-2233.e25 PubMed35750033

2022

Cholesterol and matrisome pathways dysregulated in astrocytes and microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Tcw,Lu Qian,Nina H Pipalia,Michael J Chao,Shuang A Liang,Yang Shi,Bharat R Jain,Sarah E Bertelsen,Manav Kapoor,Edoardo Marcora,Elizabeth Sikora,Elizabeth J Andrews,Alessandra C Martini,Celeste M Karch,Elizabeth Head,David M Holtzman,Bin Zhang,Minghui Wang,Frederick R Maxfield,Wayne W Poon,Alison M Goate

Bioscience reports 37: PubMed28536312

2017

Effects of ginsenoside Rg1-loaded alginate-chitosan microspheres on human bone marrow stromal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Hai Guo,Shuai Zhao,Yan-Xin Du,Qing-Jia Xing,Bo-Lai Chen,Chu-Qin Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB194325)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Product promise