Skip to main content

Mouse Recombinant Monoclonal GFAP antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-Fr, ICC/IF and reacts with Mouse, Rat samples.

Be the first to review this product! Submit a review

Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (AB313596), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (AB313596), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (AB313596), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (AB313596), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (AB313596), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-FrICC/IF
Mouse
Tested
Tested
Rat
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Confocal image showing positive staining on mouse cerebrum. Low expression: confocal image showing no staining on mouse liver. Confocal image showing positive staining on rat cerebrum. Low expression: confocal image showing no staining on rat liver.

Species
Rat
Dilution info
1/500
Notes

Confocal image showing positive staining on mouse cerebrum. Low expression: confocal image showing no staining on mouse liver. Confocal image showing positive staining on rat cerebrum. Low expression: confocal image showing no staining on rat liver.

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

Confocal image showing positive staining in mouse and rat primary glia cells.

Species
Rat
Dilution info
1/50
Notes

Confocal image showing positive staining in mouse and rat primary glia cells.

Associated Products

Select an associated product type

15 products for Alternative Version

Target data

Function

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Alternative names

Glial fibrillary acidic protein, GFAP

Recommended products

Mouse Recombinant Monoclonal GFAP antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-Fr, ICC/IF and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG1
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR1034Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

ab313596 is a mouse monoclonal chimeric antibody.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)

    GFAP Immunocytochemistry/ Immunofluorescence staining of mouse primary neural/glia cell using mouse Anti-GFAP antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling GFAP with ab313596 at 1/50 (10.0 ug/ml) dilution (Green). Confocal image showing positive staining in mouse primary glia cells.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab194324 Anti-GFAP rabbit monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/100 5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)

    GFAP Immunocytochemistry/ Immunofluorescence staining of rat primary neural/glia cell using mouse Anti-GFAP antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling GFAP with ab313596 at 1/50 (10.0 ug/ml) dilution (Green). Confocal image showing positive staining in rat primary glia cells.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab194324 Anti-GFAP rabbit monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/100 5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)

    GFAP Immunohistochemistry (Frozen sections) staining of Rat hippocampus (fresh frozen) using mouse Anti-GFAP antibody

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (fresh frozen) tissue labeling MAP2 with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 at 1/2000 (0.486 ug/ml) dilution followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution (Green).

    Panel A: merged staining of anti-MAP2 (Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993, green), anti-NeuN (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487, red) and anti-GFAP (ab313596, grey) on rat hippocampus.

    Panel B: anti-MAP2 stained on rat hippocampus.

    Panel C: anti-NeuN stained in neuron of rat hippocampus.

    Panel D: anti-GFAP stained in astrocytes of rat hippocampus.

    The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993, Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 and ab313596 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)at 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)

    GFAP Immunohistochemistry (Frozen sections) staining of Mouse hippocampus (fresh frozen) using mouse Anti-GFAP antibody

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh frozen) tissue labeling MAP2 with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 at 1/2000 (0.486 ug/ml) dilution followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution (Green).

    Panel A: merged staining of anti-MAP2 (Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993, green), anti-NeuN (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487, red) and anti-GFAP (ab313596, grey) on mouse hippocampus.

    Panel B: anti-MAP2 stained on mouse hippocampus.

    Panel C: anti-NeuN stained in neuron of mouse hippocampus.

    Panel D: anti-GFAP stained in astrocytes of mouse hippocampus.

    The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993, Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 and ab313596 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)at 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling GFAP with ab313596 at 1/500 (1.0 ug/ml) dilution (Green). Confocal image showing positive staining on mouse cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab313596 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling GFAP with ab313596 at 1/500 (1.0 ug/ml) dilution (Green). Confocal image showing positive staining on rat cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab313596 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling GFAP with ab313596 at 1/500 (1.0 ug/ml) dilution (Green). Low expression: confocal image showing no staining on mouse liver. The nuclear counterstain was DAPI (Blue). The section was incubated with ab313596 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling GFAP with ab313596 at 1/500 (1.0 ug/ml) dilution (Green). Low expression: confocal image showing no staining on rat liver. The nuclear counterstain was DAPI (Blue). The section was incubated with ab313596 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com