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Rat Recombinant Monoclonal GFAP antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, IHC-Fr and reacts with Mouse, Rat samples.

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Images

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) (AB317721), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) (AB317721), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) (AB317721), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) (AB317721), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) (AB317721), expandable thumbnail

Key facts

Isotype

IgG2a

Host species

Rat

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-Fr
Mouse
Tested
Tested
Rat
Tested
Tested

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

-

Species

Rat

Dilution info

1/50

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

-

Species

Rat

Dilution info

1/100

Notes

-

Target data

Function

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Alternative names

Recommended products

Rat Recombinant Monoclonal GFAP antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, IHC-Fr and reacts with Mouse, Rat samples.

Key facts

Isotype

IgG2a

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR1034Y

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) (ab317721), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) (ab317721)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh frozen) tissue labeling GFAP with ab317721 at 1/100 (5.0 ug/ml) dilution (Magenta).

    Low expression: confocal image showing no staining on rat liver. The nuclear counterstain was DAPI (Blue). The section was incubated with ab317721 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) (ab317721), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) (ab317721)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh frozen) tissue labeling GFAP with ab317721 at 1/100 (5.0 ug/ml) dilution (Magenta).



    Low expression: confocal image showing no staining on mouse liver. The nuclear counterstain was DAPI (Blue). The section was incubated with ab317721 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) (ab317721), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) (ab317721)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh frozen) tissue labeling GFAP with ab317721 at 1/100 (5.0 ug/ml) dilution (Magenta).



    Confocal image showing positive staining on rat cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab317721 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) (ab317721), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) (ab317721)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh frozen) tissue labeling GFAP with ab317721 at 1/100 (5.0 ug/ml) dilution (Magenta).

    Confocal image showing positive staining on mouse cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab317721 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) (ab317721), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) (ab317721)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling GFAP with ab317721 at 1/50 (10.0 ug/ml) dilution (Magenta).

    '

    Confocal image showing cytoplasmic staining in rat primary glia cell (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] ab194324 Anti-GFAP rabbit monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin (Green). The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) (ab317721), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Rat IgG2a (Chimeric) (ab317721)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling GFAP with ab317721 at 1/50 (10.0 ug/ml) dilution (Magenta).

    Confocal image showing cytoplasmic staining in mouse primary glia cell (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] ab194324 Anti-GFAP rabbit monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin (Green). The Nuclear counterstain was DAPI (Blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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