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AB325281

Alexa Fluor® 647 Anti-GFP VHH antibody [1H1 / 1B2]

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Anti-GFP VHH antibody - conjugated to Alexa Fluor® 647. Single-domain antibody raised in Alpaca, suitable for ICC/IF.

  • Enhanced penetration in 3D tissue sections & whole-mount samples
  • Superior brightness and ideal for super resolution imaging
  • Reliable and quantitative readout in all fluorescence-based analyses

View Alternative Names

Green fluorescent protein, GFP

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFP VHH antibody [1H1 / 1B2] (AB325281)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GFP VHH antibody [1H1 / 1B2] (AB325281)

PFA-fixed Cos7 cells expressing a TOM70-nfGFP-BFP fusion protein (nf : non-fluorescent) were stained with ab325281 at a 1/500 dilution. (A) Greyscale image of the stained displayed with ab325281. (B) False color representation of the image shown in A is presented in magenta (coloring according to the excitation wavelength of the employed fluorophore). (C) The corresponding BFP signal of the depicted section. (D) Merge of A and C. False color representation of A in magenta and C in blue.

Key facts

Host species

Alpaca

Clonality

Multiclonal

Clone number

1H1 / 1B2

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Aequorea victoria

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Aequorea victoria": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Aequorea victoria": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Features:

  • A blend of two high affinity monovalent sdAbs directs four fluorophores to each target protein – ideal for bright signals
  • Small size for optimal access to the respective target protein, also in crowded environment or 3D samples

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1 month
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
See full target information GFP
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com