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Rabbit Recombinant Monoclonal Glucocorticoid Receptor antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Glucocorticoid Receptor antibody [EPR19621] (AB237225), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested
Mouse
Predicted
Rat
Predicted

Tested
Tested

Species
Human
Dilution info
1/50
Notes

This product gave a positive signal in dexamethasone (100 nM 2 hours) treated HeLa fixed with 4% formaldehyde (10 min).

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

2 products for Alternative Product

Target data

Function

Receptor for glucocorticoids (GC) (PubMed:27120390, PubMed:37478846). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors (PubMed:28139699). Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed:25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity). Isoform Alpha. Has transcriptional activation and repression activity (PubMed:11435610, PubMed:15769988, PubMed:15866175, PubMed:17635946, PubMed:19141540, PubMed:19248771, PubMed:20484466, PubMed:21664385, PubMed:23820903). Mediates glucocorticoid-induced apoptosis (PubMed:23303127). Promotes accurate chromosome segregation during mitosis (PubMed:25847991). May act as a tumor suppressor (PubMed:25847991). May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression (By similarity). Isoform Beta. Acts as a dominant negative inhibitor of isoform Alpha (PubMed:20484466, PubMed:7769088, PubMed:8621628). Has intrinsic transcriptional activity independent of isoform Alpha when both isoforms are coexpressed (PubMed:19248771, PubMed:26711253). Loses this transcription modulator function on its own (PubMed:20484466). Has no hormone-binding activity (PubMed:8621628). May play a role in controlling glucose metabolism by maintaining insulin sensitivity (By similarity). Reduces hepatic gluconeogenesis through down-regulation of PEPCK in an isoform Alpha-dependent manner (PubMed:26711253). Directly regulates STAT1 expression in isoform Alpha-independent manner (PubMed:26711253). Isoform Alpha-2. Has lower transcriptional activation activity than isoform Alpha. Exerts a dominant negative effect on isoform Alpha trans-repression mechanism (PubMed:20484466). Isoform GR-P. Increases activity of isoform Alpha. Isoform Alpha-B. More effective than isoform Alpha in transcriptional activation, but not repression activity. Isoform 10. Has transcriptional activation activity. Isoform Alpha-C1. Has transcriptional activation activity. Isoform Alpha-C2. Has transcriptional activation activity. Isoform Alpha-C3. Has highest transcriptional activation activity of all isoforms created by alternative initiation (PubMed:15866175, PubMed:23820903). Has transcriptional repression activity (PubMed:23303127). Mediates glucocorticoid-induced apoptosis (PubMed:23303127, PubMed:23820903). Isoform Alpha-D1. Has transcriptional activation activity. Isoform Alpha-D2. Has transcriptional activation activity. Isoform Alpha-D3. Has lowest transcriptional activation activity of all isoforms created by alternative initiation (PubMed:15866175, PubMed:23820903). Has transcriptional repression activity (PubMed:23303127).

Alternative names

GRL, NR3C1, Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1

Recommended products

Rabbit Recombinant Monoclonal Glucocorticoid Receptor antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19621
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The glucocorticoid receptor (GR) also known as the cortisol receptor is a type of nuclear receptor that functions as a transcription factor. This receptor has a molecular mass of approximately 97 kDa. GR is expressed in various tissues including the liver lung and immune cells. Its mechanical action involves binding to glucocorticoids—hormones like cortisol—which can regulate DNA transcription. When activated the receptor translocates into the cell nucleus where it can directly interact with DNA to regulate gene expression.

Biological function summary

The glucocorticoid receptor plays a significant role in mediating the physiological effects of glucocorticoids. It controls processes like inflammation and immune response. The receptor is part of a larger receptor complex enhancing its effectiveness in gene regulation. It modulates expression levels of diverse genes involved in metabolism immune functionality and cellular growth. GR's regulatory actions make it contextual within various biological processes impacting cellular behavior extensively.

Pathways

The glucocorticoid receptor integrates into significant signaling networks like the hypothalamic-pituitary-adrenal (HPA) axis and the inflammatory response pathway. This receptor coordinates with other proteins to control stress responses and inflammatory signals. In the HPA axis GR helps regulate cortisol levels and counteracts inflammatory cytokines. Its interaction with other receptors and transcription factors exemplifies its role in essential pathways that maintain homeostasis and stress adaptation within the organism.

Associated diseases and disorders

Abnormal glucocorticoid receptor function links to conditions like Cushing's syndrome and glucocorticoid resistance. Cushing's syndrome characterized by excessive cortisol levels shows altered GR signaling. Similarly glucocorticoid resistance involves mutations or dysfunctions in GR that lead to improper hormone action affecting inflammation and immune responses. These diseases often involve other proteins such as various cytokines in the inflammatory response showing the broad impact of GR in disease processes.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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1 product image

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Glucocorticoid Receptor antibody [EPR19621] (ab237225), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Glucocorticoid Receptor antibody [EPR19621] (ab237225)

    ab237225 staining Glucocorticoid Receptor in HeLa cells +/- dexamethasone (100 nM 2 hours). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab237225 at 1/50 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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