Anti-Glucose Transporter GLUT1 [EPR3915] Alexa Fluor® 647 conjugated antibody (ab195020) rabbit monoclonal antibody that is used to detect Glucose Transporter GLUT1 in Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 20 publications
Images
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB195020), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-glucose-transporter-glut1-antibody-epr3915-ab195020--immunocytochemistry-immunofluorescence-img93756.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB195020)")
![Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB195020), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-glucose-transporter-glut1-antibody-epr3915-ab195020--flow-cytometry-intracellular-img51093.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB195020)")
Publications
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- Nature metabolism 5:1969-19852023The glucose transporter 2 regulates CD8 T cell function via environment sensing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongmei Fu,Juho Vuononvirta,Silvia Fanti,Fabrizia Bonacina,Antonio D'Amati,Guosu Wang,Thanushiyan Poobalasingam,Maria Fankhaenel,Davide Lucchesi,Rachel Coleby,David Tarussio,Bernard Thorens,Robert J Hearnden,M Paula Longhi,Paul Grevitt,Madeeha H Sheikh,Egle Solito,Susana A Godinho,Michele Bombardieri,David M Smith,Dianne Cooper,Asif J Iqbal,Jeffrey C Rathmell,Samuel Schaefer,Valle Morales,Katiuscia Bianchi,Giuseppe Danilo Norata,Federica M Marelli-BergPubMed 37884694
- JCI insight 8:2023T cell metabolic reprogramming in acute kidney injury and protection by glutamine blockade.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyungho Lee,Elizabeth A Thompson,Sepideh Gharaie,Chirag H Patel,Johanna T Kurzhagen,Phillip M Pierorazio,Lois J Arend,Ajit G Thomas,Sanjeev Noel,Barbara S Slusher,Hamid RabbPubMed 37166984
- Nature cell biology 25:740-7532023Tissue memory relies on stem cell priming in distal undamaged areas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChiara Levra Levron,Mika Watanabe,Valentina Proserpio,Gabriele Piacenti,Andrea Lauria,Stefan Kaltenbach,Annalaura Tamburrini,Takuma Nohara,Francesca Anselmi,Carlotta Duval,Luca Elettrico,Daniela Donna,Laura Conti,Denis Baev,Ken Natsuga,Tzachi Hagai,Salvatore Oliviero,Giacomo DonatiPubMed 37081165
- eLife 12:2023Molecular characterization of the intact mouse muscle spindle using a multi-omics approach.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBavat Bornstein,Lia Heinemann-Yerushalmi,Sharon Krief,Ruth Adler,Bareket Dassa,Dena Leshkowitz,Minchul Kim,Guy Bewick,Robert W Banks,Elazar ZelzerPubMed 36744866
- Science immunology 7:eabq74322022cDC1 coordinate innate and adaptive responses in the omentum required for T cell priming and memory.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid A Christian,Thomas A Adams,Lindsey A Shallberg,Anthony T Phan,Tony E Smith,Mosana Abraha,Joseph Perry,Gordon Ruthel,Joseph T Clark,Gretchen Harms Pritchard,Lillian R Aronson,Selamawit Gossa,Dorian B McGavern,Ross M Kedl,Christopher A HunterPubMed 36179012
- Circulation research 130:779-7812022Diabetes Suppresses Glucose Uptake and Glycolysis in Macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunosuke Matsuura,Masami Shimizu-Albergine,Shelley Barnhart,Farah Kramer,Cheng-Chieh Hsu,Vishal Kothari,Jingjing Tang,Sina A Gharib,Jenny E Kanter,E Dale Abel,Rong Tian,Baohai Shao,Karin E BornfeldtPubMed 35170337
- Cells 11:2022Dysregulated PI3K Signaling in B Cells of CVID Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIna Harder,Matthias Münchhalfen,Geoffroy Andrieux,Melanie Boerries,Bodo Grimbacher,Hermann Eibel,Maria Elena Maccari,Stephan Ehl,Jürgen Wienands,Julia Jellusova,Klaus Warnatz,Baerbel KellerPubMed 35159274
- Viruses 14:2022Metabolic Reprogramming of Glioblastoma Cells during HCMV Infection Induces Secretome-Mediated Paracrine Effects in the Microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMark A A Harrison,Emily M Hochreiner,Brooke P Benjamin,Sean E Lawler,Kevin J ZwezdarykPubMed 35062307
- Cell reports. Medicine 2:1004572021Synergistic T cell signaling by 41BB and CD28 is optimally achieved by membrane proximal positioning within parallel chimeric antigen receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTamara Muliaditan,Leena Halim,Lynsey M Whilding,Benjamin Draper,Daniela Y Achkova,Fahima Kausar,Maya Glover,Natasha Bechman,Appitha Arulappu,Jenifer Sanchez,Katie R Flaherty,Jana Obajdin,Kristiana Grigoriadis,Pierre Antoine,Daniel Larcombe-Young,Caroline M Hull,Richard Buus,Peter Gordon,Anita Grigoriadis,David M Davies,Anna Schurich,John MaherPubMed 35028604
- Advanced biology 5:e21006092021Microfluidic Co-Culture Platform to Recapitulate the Maternal-Placental-Embryonic Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulia A Boos,Patrick M Misun,Giulia Brunoldi,Lea A Furer,Leonie Aengenheister,Mario Modena,Nassim Rousset,Tina Buerki-Thurnherr,Andreas HierlemannPubMed 34145989
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt (Intra) | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes ab199093 - Rabbit monoclonal IgG (Alexa Fluor®; 647), is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
7 products for Alternative Version
Target data
Function
Facilitative glucose transporter, which is responsible for constitutive or basal glucose uptake (PubMed:10227690, PubMed:10954735, PubMed:18245775, PubMed:19449892, PubMed:25982116, PubMed:27078104, PubMed:32860739). Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses (PubMed:18245775, PubMed:19449892). Most important energy carrier of the brain: present at the blood-brain barrier and assures the energy-independent, facilitative transport of glucose into the brain (PubMed:10227690). In association with BSG and NXNL1, promotes retinal cone survival by increasing glucose uptake into photoreceptors (By similarity). Required for mesendoderm differentiation (By similarity).
Alternative names
GLUT1, SLC2A1, HepG2 glucose transporter, GLUT-1
Recommended products
Anti-Glucose Transporter GLUT1 [EPR3915] Alexa Fluor® 647 conjugated antibody (ab195020) rabbit monoclonal antibody that is used to detect Glucose Transporter GLUT1 in Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 20 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR3915
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 7.7 x 10-12 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195020) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF), in Human samples.
Recommended positive controls
ICC/IF: HepG2 cells.Flow Cyt (intra): HepG2 cells.
Trusted by the scientific community
Anti-Glucose Transporter GLUT1 [EPR3915] Alexa Fluor® 647 (ab195020) was first used in a scientific publication in 2014 and has been cited over 20 times in peer-reviewed journals.
Reviewed by scientists
Anti-Glucose Transporter GLUT1 [EPR3915] Alexa Fluor® 647 (ab195020) has over 5 independent reviews from customers.
Related products
Antibody clone EPR3915 is also available pre-conjugated to a variety of labels for your convenience – Anti-Glucose Transporter GLUT1 Alexa Fluor® 647 [EPR3915] (ab195020).
Other related products
We have a range of other formats of antibody clone [EPR3915] also available for your convenience:
Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730, Alexa Fluor® 647 - ab195020, HRP - HRP Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab195021, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab195359, Carrier free - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - Low endotoxin, Azide free ab196357, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab206360, PE - PE Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab209449, Alexa Fluor® 405 - Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab210438, Carrier free - Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free ab252403, APC - APC Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab316298, FITC - FITC Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab322306, Biotin - Biotin Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab323677
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Glucose Transporter GLUT1 also known as SLC2A1 is an important protein responsible for the transport of glucose across cell membranes. The GLUT1 transporter has a molecular weight of approximately 55 kDa. This protein is highly expressed in erythrocytes endothelial cells lining blood vessels and in the blood-brain barrier. Its primary role is to facilitate the basal glucose uptake necessary for cellular metabolism particularly in tissues where glucose is a critical energy source.
- Biological function summary
This glucose transporter plays a significant role in maintaining glucose homeostasis in the human body. GLUT1 functions independently and not as part of a complex. It ensures that glucose is available to cells with high metabolic demands including the brain and red blood cells where it remains important for survival and function. Its expression level can be influenced by various factors including hypoxia and insulin.
- Pathways
GLUT1 is involved in the glycolysis and hypoxia-related pathways. It supports the glycolytic pathway by ensuring a sufficient supply of glucose to the cells which is then metabolized to produce ATP. Additionally during hypoxic conditions GLUT1 expression can increase aligning with proteins like HIF-1α which helps cells adapt by modifying their metabolism. This coordinated regulation permits cells to adjust their energy systems according to the oxygen availability.
- Associated diseases and disorders
GLUT1 is implicated in glucose transporter type 1 deficiency syndrome (GLUT1 DS) and various forms of cancer. GLUT1 DS results from inadequate glucose transport into the brain presenting neurological symptoms due to energy deficiency. In cancer overexpression of GLUT1 links to increased glucose uptake and tumor growth a condition known to involve proteins like hexokinase. These associations underline GLUT1's contribution to both genetic defects and metabolic shifts in cancerous tissues.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195020), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-glucose-transporter-glut1-antibody-epr3915-ab195020--immunocytochemistry-immunofluorescence-img93756.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195020)")
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195020)
ab195020 staining Glucose Transporter GLUT1 in HepG2 (human liver hepatocellular carcinoma cell line) cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab195020 at a working dilution of 1 in 50 (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 2μg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
This product gave a positive signal in 100% methanol (5 min) fixed HepG2 cells under the same testing conditions.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
![Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195020), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-glucose-transporter-glut1-antibody-epr3915-ab195020--flow-cytometry-intracellular-img51093.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195020)")
Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195020)
Overlay histogram showing HepG2 (human liver hepatocellular carcinoma cell line) cells stained with ab195020 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195020, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter.
This antibody gave a positive signal in HepG2 fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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