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AB310124

Alexa Fluor® 647 Anti-GLUD1 + GLUD2 antibody [EPR11370]

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Rabbit Recombinant Monoclonal GLUD1 antibody - conjugated to Alexa Fluor® 647.

View Alternative Names

GLUD, GLUD1, GDH 1

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-GLUD1 + GLUD2 antibody [EPR11370]

  • 578 PE

    PE Anti-GLUD1 + GLUD2 antibody [EPR11370]

  • 660 APC

    APC Anti-GLUD1 + GLUD2 antibody [EPR11370]

  • HRP

    HRP Anti-GLUD1 + GLUD2 antibody [EPR11370]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-GLUD1 + GLUD2 antibody [EPR11370]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-GLUD1 + GLUD2 antibody [EPR11370]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-GLUD1 + GLUD2 antibody [EPR11370]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-GLUD1 + GLUD2 antibody [EPR11370]

  • Unconjugated

    Anti-GLUD1 + GLUD2 antibody [EPR11370]

  • Carrier free

    Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11370

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GLUD1 and GLUD2 also known as glutamate dehydrogenases 1 and 2 are enzymes that convert glutamate to α-ketoglutarate linking amino acid metabolism with the tricarboxylic acid cycle. GLUD1 has a mass of approximately 56 kDa while GLUD2 has a similar mass range. GLUD1 expresses ubiquitously in most tissues including the liver and brain whereas GLUD2 predominantly expresses in the brain and testes. These enzymes play a significant role in cellular energy metabolism and neurotransmitter regulation.
Biological function summary

GLUD1 and GLUD2 enzymes act in mitochondrial processes where they catalyze oxidative deamination of glutamate. This reaction facilitates removal of excess nitrogen and supports energy balance within cells. These enzymes do not form part of larger complexes but operate independently within the mitochondrial matrix. The differential expression of GLUD1 and GLUD2 suggests specialized roles in nervous system metabolism with GLUD2 potentially adapting to higher demand for neurotransmitter turnover in the brain.

Pathways

Components including GLUD1 and GLUD2 participate in the glutamate metabolism pathway and the urea cycle intimately linking key points of nitrogen and carbon metabolism. These enzymes influence the tricarboxylic acid cycle by producing α-ketoglutarate a critical molecule. Their activity intersects with proteins such as IDH1 and IDH2 isocitrate dehydrogenases that also alter tricarboxylic acid cycle fluxes coordinating cellular responses to metabolic needs and stress conditions.

GLUD1 and GLUD2 mutations can connect to hyperinsulinism/hyperammonemia syndrome where dysregulated enzyme activity leads to abnormal ammonia and insulin levels. Their role in the nervous system connects them to neurodegenerative disorders like Alzheimer's disease where altered glutamate metabolism may contribute to disease progression. The relationship with proteins like APP (amyloid precursor protein) emphasizes the broader implications of GLUD pathways in neural tissue health and disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle (PubMed : 11032875, PubMed : 11254391, PubMed : 16023112, PubMed : 16959573). Plays a role in insulin homeostasis (PubMed : 11297618, PubMed : 9571255). May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate (By similarity).
See full target information GLUD1

Additional targets

GLUD2
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Product promise

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