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AB326127

Alexa Fluor® 647 Anti-Glycogen synthase 1/GYS1 antibody [EP817Y]

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Rabbit Recombinant Monoclonal Glycogen synthase 1/GYS1 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

GYS, GYS1, Glycogen synthase 1

1 Images
Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (AB326127)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (AB326127)

Flow cytometry overlay histogram showing wild-type HeLa (green line) and GYS1 knockout HeLa stained with ab326127 (magenta line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab326127) (1x 106 in 100µl at 0.008 µg/ml (1/62500)) for 30min at 22°C.Isotype control antibody was Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (black line) in HeLa WT cells (black line) and HeLa-GYS1 KO cells (grey line), used at the same concentration and conditions as the primary antibody. Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.This antibody gave a positive signal in wild-type HeLa fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.This data was acquired using a Beckman Coulter CytoFlexLX flow cytometer.

  • Carrier free

    Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] - BSA and Azide free

  • Unconjugated

    Anti-Glycogen synthase 1/GYS1 antibody [EP817Y]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Glycogen synthase 1/GYS1 antibody [EP817Y]

  • 578 PE

    PE Anti-Glycogen synthase 1/GYS1 antibody [EP817Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP817Y

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/62500", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycogen synthase 1 (GYS1) also known as muscle glycogen synthase catalyzes the synthesis of glycogen from glucose. It adds glucose units from UDP-glucose to the non-reducing ends of glycogen chains facilitating glycogen elongation. The molecular mass of GYS1 is approximately 85 kDa. It is predominantly expressed in skeletal muscle tissue providing energy storage primarily in the form of glycogen. GYS1 is regulated through phosphorylation wherein it undergoes inactive to active conformational changes.
Biological function summary

This enzyme plays a critical role in the energy storage mechanism of muscle tissues. GYS1 functions as a part of the glycogen synthase complex interacting with various glycogen-associated proteins to modulate its activity. These interactions control the enzyme's affinity for its substrate and its susceptibility to regulatory signals like phosphorylation affecting glycogen synthesis efficiency. Through its activity GYS1 ensures a readily available reserve of glucose in muscle tissues for rapid energy production when required.

Pathways

Glycogen synthase 1 is a central component of the glycogen metabolism pathway. It acts alongside other enzymes such as glycogen phosphorylase which catalyzes glycogen breakdown. The balance between GYS1 and glycogen phosphorylase determines the rate of glycogen turnover within cells. GYS1 is further regulated by the insulin signaling pathway where insulin promotes glycogen synthesis by altering GYS1 activity alongside proteins like AKT influencing glucose homeostasis and energy management in tissues.

Dysregulation of GYS1 activity often associates with glycogen storage diseases notably Type 0 where deficiencies in glycogen storage lead to episodes of low blood sugar and reduced exercise capacity. Furthermore aberrations in GYS1 function contribute to insulin resistance and Type 2 diabetes conditions linked with impaired glucose metabolism. Altered GYS1 activity impacts proteins like glycogen phosphorylase affecting overall glycogen metabolism balance and contributing to metabolic disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Glycogen synthase participates in the glycogen biosynthetic process along with glycogenin and glycogen branching enzyme. Extends the primer composed of a few glucose units formed by glycogenin by adding new glucose units to it. In this context, glycogen synthase transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
See full target information GYS1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com