AB237290

Alexa Fluor® 647 Anti-Glypican 1/ GPC1 antibody [EPR19285]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal Glypican 1/ GPC1 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Glypican-1, GPC1

2 Images

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Glypican 1/ GPC1 antibody [EPR19285] (AB237290)

ab237290 staining Glypican 1/ GPC1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab237290 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Glypican 1/ GPC1 antibody [EPR19285] (AB237290)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Glypican 1/ GPC1 antibody [EPR19285] (AB237290)

Overlay histogram showing MCF7 cells stained with ab237290 (red line).

The cells were incubated in 1x PBS/10% normal Goat serum to block non-specific protein-protein interactions followed by the antibody (ab237290) (1x10⁶ in 100 μl at 1 μg/ml (1/500)) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor^® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

660 APC

APC Anti-Glypican 1/ GPC1 antibody [EPR19285]
578 PE

PE Anti-Glypican 1/ GPC1 antibody [EPR19285]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Glypican 1/ GPC1 antibody [EPR19285]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Glypican 1/ GPC1 antibody [EPR19285]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Glypican 1/ GPC1 antibody [EPR19285]
Unconjugated

Anti-Glypican 1/ GPC1 antibody [EPR19285]
Carrier free

Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19285

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa fixed with 100% methanol (5 min).</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glypican 1 (GPC1) also known as glypican-1 is a heparan sulfate proteoglycan anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor. This protein belongs to the glypican family and has an approximate mass of 60 to 70 kDa. GPC1 is found mainly on the surface of cells in the central nervous system as well as in muscle tissues and certain epithelial cells. Its structure facilitates interaction with cell surface receptors and extracellular matrix components playing an important role in cellular communication and signaling processes.

Biological function summary

GPC1 influences the regulation of growth factors and morphogens and it has a significant role in the cell division and growth processes. It is not recognized as part of any larger protein complex but interacts with diverse ligand molecules. Researchers identify GPC1 as an important facilitator of fibroblast growth factors (FGF) and sonic hedgehog (Shh) signaling. Its interaction with molecular partners helps to regulate developmental signals important for proper cellular function and organ development.

Pathways

The contributions of GPC1 are observed in the hedgehog signaling pathway and fibroblast growth factor signaling. GPC1's interaction within the hedgehog pathway is critical for organizing cellular growth and differentiation while its involvement in the FGF pathway supports angiogenesis and wound healing. GPC1 coordinates with other glypican family members and related proteins like FGFR1 to mediate these signaling processes effectively.

Researchers have linked GPC1 with cancer progression and neurodegenerative diseases. Abnormal GPC1 expression is often noted in pancreatic cancer influencing tumor growth and metastasis through its modulation of growth factor signaling. Moreover its role in neurodegenerative conditions such as Alzheimer's disease establishes connections with amyloid-beta peptides and tau proteins contributing to disease pathogenesis. These associations make GPC1 an intriguing target for therapeutic intervention and a focus for ongoing medical research.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination (By similarity). May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling.

See full target information GPC1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 27:1938-1951 PubMed41028835

2025

Phase-separated NDF-FACT condensates facilitate transcription elongation on chromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Ziwei Li,Francesca Burgos-Bravo,Kevin Xu,Chen Li,Kelvin Y Kwan,Alexander B Tong,Zelin Shan,Huan Wang,Motoki Takaku,Joey Li,Zheng Shi,Dmitry Lyumkis,Carlos Bustamante,Jia Fei

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2306373 PubMed38204202

2024

Extracellular Vesicular Analysis of Glypican 1 mRNA and Protein for Pancreatic Cancer Diagnosis and Prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Li,Chi-Ling Chiang,Kwang Joo Kwak,Xinyu Wang,Sital Doddi,Lakshmi V Ramanathan,Sun M Cho,Ya-Chin Hou,Tai-Shan Cheng,Xiaokui Mo,Yueh-Shih Chang,Hui-Lan Chang,Weiming Cheng,Wei-Ni Tsai,Luong T H Nguyen,Junjie Pan,Yifan Ma,Xilal Y Rima,Jingjing Zhang,Eduardo Reategui,Yeh-Shiu Chu,Peter Mu-Hsin Chang,Pei-Hung Chang,Chi-Ying F Huang,Cheng-Hsu Wang,Yan-Shen Shan,Chung-Pin Li,Martin Fleisher,L James Lee

ACS nano 17:14619-14631 PubMed37470391

2023

Graphene Sensor Arrays for Rapid and Accurate Detection of Pancreatic Cancer Exosomes in Patients' Blood Plasma Samples.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyi Yin,Lizhou Xu,Bruno Gil,Nabeel Merali,Maria S Sokolikova,David C A Gaboriau,Daniel S K Liu,Ahmad Nizamuddin Muhammad Mustafa,Sarah Alodan,Michael Chen,Oihana Txoperena,María Arrastua,Juan Manuel Gomez,Nerea Ontoso,Marta Elicegui,Elias Torres,Danyang Li,Cecilia Mattevi,Adam E Frampton,Long R Jiao,Sami Ramadan,Norbert Klein

Cancers 14: PubMed35884424

2022

Isolation of Circulating Tumor Cells from Seminal Fluid of Patients with Prostate Cancer Using Inertial Microfluidics.

Applications

Unspecified application

Species

Unspecified reactive species

Alexey S Rzhevskiy,Alina Y Kapitannikova,Steven A Vasilescu,Tamilla A Karashaeva,Sajad Razavi Bazaz,Mark S Taratkin,Dmitry V Enikeev,Vladimir Y Lekarev,Evgeniy V Shpot,Denis V Butnaru,Sergey M Deyev,Jean Paul Thiery,Andrei V Zvyagin,Majid Ebrahimi Warkiani

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-Glypican 1/ GPC1 antibody [EPR19285]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Glypican 1/ GPC1 antibody [EPR19285] (AB237290)

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Glypican 1/ GPC1 antibody [EPR19285] (AB237290)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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