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Rabbit Recombinant Monoclonal GM130 antibody - conjugated to Alexa Fluor® 647. cis-Golgi marker. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.


Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (AB195303), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (AB195303), expandable thumbnail
  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (AB195303), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow Cyt (Intra)
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/100 - 1/2500
Notes

-

Tested
Tested

Species
Human
Dilution info
0.2 µL for 106 Cells
Notes

ab199093 - Rabbit monoclonal IgG (Alexa Fluor® 647), is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

2 products for Alternative Product

2 products for Alternative Version

Target data

Function

Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (Probable) (PubMed:16489344). Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane (By similarity). Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity). Also plays a key role in spindle pole assembly and centrosome organization (PubMed:26165940). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (PubMed:19242490, PubMed:26165940). Regulates the meiotic spindle pole assembly, probably via the same mechanism (By similarity). Also regulates the centrosome organization (PubMed:18045989, PubMed:19109421). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (PubMed:16489344, PubMed:17314401).

Alternative names

Golgin subfamily A member 2, 130 kDa cis-Golgi matrix protein, GM130 autoantigen, Golgin-95, GM130, GOLGA2

Recommended products

Rabbit Recombinant Monoclonal GM130 antibody - conjugated to Alexa Fluor® 647. cis-Golgi marker. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP892Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

GM130 also known as Golgi matrix protein 130 kDa or GM-130 plays an important role in the organization of the Golgi apparatus. The GM130 protein is vital for maintaining the structure of the Golgi serving as a Golgi marker for researchers. Its molecular weight is approximately 130 kDa. You will commonly find GM130 expressed in cells localizing to the cis-Golgi network. As a Golgi marker it serves as an indication of the integrity and positioning of the Golgi apparatus in the cell.

Biological function summary

GM130 serves as a structural component within the Golgi matrix. It interacts with other proteins such as GRASP65 to form a complex which is essential for Golgi stacking and maintenance of Golgi architecture. This interaction helps regulate transport processes inside cells. GM130 proteins help formation of mini-stacks and transitioning of vesicles. In cells GM130 plays a significant role to maintain efficient protein trafficking through the Golgi network.

Pathways

GM130 interacts with proteins like Rab1 during the vesicular transport pathway. It plays a part in the transport of proteins from the endoplasmic reticulum to the Golgi apparatus. Another related pathway involves SNARE proteins whereby GM130 contributes to membrane docking and fusion events. Through these interactions GM130 modulates the fidelity and specificity of molecular cargo transport within the cell.

Associated diseases and disorders

GM130 has a connection to neurodegenerative diseases such as frontotemporal dementia. Disruption in GM130 proteins affects Golgi structure and function potentially leading to protein trafficking defects associated with the disorder. GM130 also relates to Alzheimer's disease through its interaction with tau protein. Alterations in GM130's function can influence tau pathology contributing to disease progression.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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3 product images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab195303), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab195303)

    ab195303 staining GM130 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab195303 at 5μg/ml (shown in red) and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1/100 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Mouse secondary (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 2μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    This product gave a positive signal in 4% formaldehyde (10min) fixed HeLa cells under the same testing conditions.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab195303), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab195303)

    Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling GM130 with ab195303. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab195303 at 1/2500 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab195303), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab195303)

    Overlay histogram showing HeLa cells stained with ab195303 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195303, 0.1μg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (Alexa® 647) (0.2μl/1x106 cells) for 30 min at 22°C. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 25mW red solid state diode laser (635nm) and 675/30 bandpass filter.

    This antibody gave a positive signal in HeLa fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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