Skip to main content

Rabbit Recombinant Monoclonal GPCR GPRC5D antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Transfected cell line - Human, Human samples.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GPCR GPRC5D antibody [EPR28376-41] (AB323465), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GPCR GPRC5D antibody [EPR28376-41] (AB323465), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GPCR GPRC5D antibody [EPR28376-41] (AB323465), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested
Transfected cell line - Human
Tested

Tested
Tested

Species

Transfected cell line - Human

Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species

Human

Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Alternative names

G-protein coupled receptor family C group 5 member D, GPRC5D

Rabbit Recombinant Monoclonal GPCR GPRC5D antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Transfected cell line - Human, Human samples.

Key facts

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR28376-41

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The GPCR GPRC5D also known as the GPRC5D protein or by the code 6D9 in certain research contexts is a member of the G-protein-coupled receptors (GPCRs) superfamily. This family acts as a significant mediator of cellular responses to external signals. The GPRC5D has a mass of approximately 56 kDa. It is mainly expressed in the human bone marrow and the keratinocytes of the skin with notable absence in other tissues. It also appears in certain immune cells indicating its potential involvement in immune system responses.

Biological function summary

The role of GPCR GPRC5D centers on cell signaling mechanisms within the immune system. GPCR GPRC5D engages in intracellular signaling cascades that modulate immune functions although it is not known to be part of a larger receptor complex. Its function may involve interactions with molecules that transmit environmental signals into cellular responses enhancing its importance in immune regulation and possibly other cellular functions.

Pathways

GPCR GPRC5D participates in signaling pathways like the immune regulation pathway and the skin development pathway. It relates to proteins such as arrestins which link GPCRs to downstream signaling pathways. This connection hints at a regulatory role in cellular response where GPCR GPRC5D acts as a conduit for signal transduction balancing cellular responses to external stimuli and maintaining cellular homeostasis.

Associated diseases and disorders

GPCR GPRC5D has associations with multiple myeloma and psoriasis. Its expression in bone marrow links it to multiple myeloma where its level might impact cancer progression. Proteins such as interleukin-17 and others relevant to psoriasis are connected to GPCR GPRC5D through skin-related pathways suggesting its role in inflammatory responses and potential as a therapeutic target for skin disorders. This illustrates GPRC5D's relevance in specific pathological conditions offering avenues for research into targeted treatments.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GPCR GPRC5D antibody [EPR28376-41] (ab323465), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GPCR GPRC5D antibody [EPR28376-41] (ab323465)

    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a human GPCR GPRC5D expression vector containing a Myc tag. (B) HEK-293T transfected with empty vector containing a Myc tag. tissue labeling GPCR GPRC5D with ab323465 at 1/100 (5.0 ug/ml) dilution.

    Positive staining on (A) HEK-293T transfected with a human GPCR GPRC5D expression vector containing a Myc tag, no staining on (B) HEK-293T transfected with empty vector containing a Myc tag.

    The section was incubated with ab323465 for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GPCR GPRC5D antibody [EPR28376-41] (ab323465), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GPCR GPRC5D antibody [EPR28376-41] (ab323465)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling GPCR GPRC5D with ab323465 at 1/50 (10.0 ug/ml) dilution.

    Negative control: no staining on human cerebrum.

    The section was incubated with ab323465 for 60 mins at room temperature. Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GPCR GPRC5D antibody [EPR28376-41] (ab323465), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GPCR GPRC5D antibody [EPR28376-41] (ab323465)

    Immunohistochemical analysis of paraffin-embedded Human hair follicle tissue labeling GPCR GPRC5D with ab323465 at 1/50 (10.0 ug/ml) dilution.

    Positive staining on human hair follicle (PMID: 30918115).

    The section was incubated with ab323465 for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com