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AB323465

Alexa Fluor® 647 Anti-GPCR GPRC5D antibody [EPR28376-41]

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Rabbit Recombinant Monoclonal GPCR GPRC5D antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Transfected cell line - Human, Human samples.

View Alternative Names

G-protein coupled receptor family C group 5 member D, GPRC5D

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GPCR GPRC5D antibody [EPR28376-41] (AB323465)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GPCR GPRC5D antibody [EPR28376-41] (AB323465)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling GPCR GPRC5D with ab323465 at 1/50 (10.0 ug/ml) dilution.

Negative control : no staining on human cerebrum.
The section was incubated with ab323465 for 60 mins at room temperature. Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GPCR GPRC5D antibody [EPR28376-41] (AB323465)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GPCR GPRC5D antibody [EPR28376-41] (AB323465)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a human GPCR GPRC5D expression vector containing a Myc tag. (B) HEK-293T transfected with empty vector containing a Myc tag. tissue labeling GPCR GPRC5D with ab323465 at 1/100 (5.0 ug/ml) dilution.

Positive staining on (A) HEK-293T transfected with a human GPCR GPRC5D expression vector containing a Myc tag, no staining on (B) HEK-293T transfected with empty vector containing a Myc tag.
The section was incubated with ab323465 for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GPCR GPRC5D antibody [EPR28376-41] (AB323465)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-GPCR GPRC5D antibody [EPR28376-41] (AB323465)

Immunohistochemical analysis of paraffin-embedded Human hair follicle tissue labeling GPCR GPRC5D with ab323465 at 1/50 (10.0 ug/ml) dilution.

Positive staining on human hair follicle (PMID : 30918115).
The section was incubated with ab323465 for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • 660 APC

    APC Anti-GPCR GPRC5D antibody [EPR28376-41]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-GPCR GPRC5D antibody [EPR28376-41]

  • Unconjugated

    Anti-GPCR GPRC5D antibody [EPR28376-41]

  • Carrier free

    Anti-GPCR GPRC5D antibody [EPR28376-41] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28376-41

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Transfected cell line - Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GPCR GPRC5D also known as the GPRC5D protein or by the code 6D9 in certain research contexts is a member of the G-protein-coupled receptors (GPCRs) superfamily. This family acts as a significant mediator of cellular responses to external signals. The GPRC5D has a mass of approximately 56 kDa. It is mainly expressed in the human bone marrow and the keratinocytes of the skin with notable absence in other tissues. It also appears in certain immune cells indicating its potential involvement in immune system responses.
Biological function summary

The role of GPCR GPRC5D centers on cell signaling mechanisms within the immune system. GPCR GPRC5D engages in intracellular signaling cascades that modulate immune functions although it is not known to be part of a larger receptor complex. Its function may involve interactions with molecules that transmit environmental signals into cellular responses enhancing its importance in immune regulation and possibly other cellular functions.

Pathways

GPCR GPRC5D participates in signaling pathways like the immune regulation pathway and the skin development pathway. It relates to proteins such as arrestins which link GPCRs to downstream signaling pathways. This connection hints at a regulatory role in cellular response where GPCR GPRC5D acts as a conduit for signal transduction balancing cellular responses to external stimuli and maintaining cellular homeostasis.

GPCR GPRC5D has associations with multiple myeloma and psoriasis. Its expression in bone marrow links it to multiple myeloma where its level might impact cancer progression. Proteins such as interleukin-17 and others relevant to psoriasis are connected to GPCR GPRC5D through skin-related pathways suggesting its role in inflammatory responses and potential as a therapeutic target for skin disorders. This illustrates GPRC5D's relevance in specific pathological conditions offering avenues for research into targeted treatments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information GPRC5D
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com