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AB203154

Alexa Fluor® 647 Anti-GST3 / GST pi antibody [EPR8263]

4

(1 Review)

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(1 Publication)

Rabbit Recombinant Monoclonal GST3 / GST pi antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

FAEES3, GST3, GSTP1, Glutathione S-transferase P, GST class-pi, GSTP1-1

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GST3 / GST pi antibody [EPR8263] (AB203154)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-GST3 / GST pi antibody [EPR8263] (AB203154)

ab203154 staining GST3 / GST pi in K562 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203154 at 1/50 dilution (shown in red). Cell membranes were labelled with WGA (Alexa Fluor® 488, shown in green). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • HRP

    HRP Anti-GST3 / GST pi antibody [EPR8263]

  • 660 APC

    APC Anti-GST3 / GST pi antibody [EPR8263]

  • 578 PE

    PE Anti-GST3 / GST pi antibody [EPR8263]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-GST3 / GST pi antibody [EPR8263]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-GST3 / GST pi antibody [EPR8263]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-GST3 / GST pi antibody [EPR8263]

  • Unconjugated

    Anti-GST3 / GST pi antibody [EPR8263]

  • Carrier free

    Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8263

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB242014

Anti-GST3 / GST pi antibody [EPR20554] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR20554] - BSA and Azide free (AB242014)

  • 0
  • 0
Primary Antibodies

AB259373

Anti-GST3 / GST pi antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB259373)

  • 0
  • 0
Primary Antibodies

AB134934

Anti-GST3 / GST pi antibody [EPR8262]

RabMAb|Recombinant|KO Validated

Western blot - Anti-GST3 / GST pi antibody [EPR8262] (AB134934)

  • 0
  • 0
Primary Antibodies

AB241331

Anti-GST3 / GST pi antibody [EPR20554]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR20554] (AB241331)

  • 0
  • 0

Complementary Products

Assay Kits

AB277423

Human GST3 / GST pi ELISA Kit

ELISA - Human GST3 / GST pi ELISA Kit (AB277423)

  • 0
  • 0
Primary Antibodies

AB3416

HRP Anti-GST antibody

ELISA - HRP Anti-GST antibody (AB3416)

  • 5
  • 3
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB78316

Anti-p53 (acetyl K120) antibody [10E5]

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K120) antibody [10E5] (AB78316)

  • 4
  • 4
Biochemicals

AB145459

Green CMFDA (5-Chloromethylfluorescein diacetate), Green fluorescent dye

Chemical Structure - Green CMFDA (5-Chloromethylfluorescein diacetate), Green fluorescent dye (AB145459)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p>This product gave a positive signal in K562 cells fixed with 100% methanol (5 min).</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GST3 also known as GST pi or GSTP is a protein part of the glutathione S-transferase family. These enzymes are involved in detoxifying processes by catalyzing the conjugation of glutathione to harmful substances. GSTP is approximately 23 kDa in size and is predominantly expressed in mammalian tissues including the liver and lung. These tissues play active roles in detoxifying external chemicals and managing oxidative stress in the body.
Biological function summary

GST3 contributes to the detoxification of xenobiotics and electrophilic compounds playing a protective role against cellular damage. It can exist as homo-dimers or form hetero-dimers with other GST family members participating in larger enzyme complexes. Through conjugation reactions GSTP helps in the detoxification processes thereby aiding cellular defenses and maintaining cellular redox balance.

Pathways

GST3 is an integral part of the phase II detoxification pathway and oxidative stress response. It interacts closely with other proteins such as Nrf2 (Nuclear factor erythroid 2-related factor 2) and helps regulate the transcription of antioxidant proteins that protect against oxidative damage. This enzyme plays a role in modulating the MAPK signaling pathway which influences cell proliferation and apoptosis.

GSTP has links to several cancer types including liver and lung cancer. Its overexpression in tumors suggests its role in cancer cell survival and resistance to chemotherapy. GSTP interacts with cancer-associated proteins like c-Jun N-terminal kinase (JNK) influencing pathways that lead to aberrant cellular growth and transformation. It also has associations with neurodegenerative diseases given its role in counteracting oxidative stress which commonly underpins neurodegeneration.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed : 9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed : 21046276). Negatively regulates CDK5 activity via p25/p35 translocation to prevent neurodegeneration.
See full target information GSTP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biology methods & protocols 10:bpaf056 PubMed40809868

2025

Innovative approach for the qualitative-quantitative assessment of neurodevelopment biomarkers research in placenta tissue using immunohistochemistry digital image analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline Camilo,Luana Martos Vieira,Gisele Rodrigues Gouveia,Arleti Caramori Torrezan,Andrea Peixoto,Veronica Euclydes,Rossana Pulcineli Vieira Francisco,Alexandra Brentani,Aloisio Felipe-Silva,Helena Brentani
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com