AB270111

Alexa Fluor® 647 Anti-HAND2 antibody [EPR19451]

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal HAND2 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC and reacts with Mouse samples.

View Alternative Names

Dhand, Hed, Thing2, Hand2, Heart- and neural crest derivatives-expressed protein 2, Helix-loop-helix transcription factor expressed in embryo and deciduum, Thing-2, dHAND

1 Images

Lab

Immunocytochemistry - Alexa Fluor® 647 Anti-HAND2 antibody [EPR19451] (AB270111)

ab270111 staining HAND2 in Neuro2a (Mouse neuroblastoma cell line) cells.

The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab270111 at 1/500 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19451

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Mouse

Applications

ICC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/500", "ICC-species-notes": "<p>This product gave a positive signal in Neuro2a fixed with 4% formaldehyde (10 min).</p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HAND2 also known as Heart and Neural Crest Derivatives Expressed 2 is a transcription factor weighing approximately 22 kDa. This protein plays an important role in regulating the expression of genes necessary for specific cell differentiation processes. HAND2 is widely expressed in cardiac and neural tissues with a particular emphasis on the developing heart and nervous system. It belongs to the basic helix-loop-helix (bHLH) family of transcription factors which are known for their function in binding to E-box sequences in DNA and controlling gene expression.

Biological function summary

HAND2 influences cardiac morphogenesis and contributes to neural crest cell migration. It engages in complex interactions with other transcription factors to modulate the expression of genes involved in these processes. HAND2 operates as part of a transcriptional complex often forming heterodimers with other bHLH proteins like E47 and HEB which enhances its DNA-binding capability and specificity. This interaction is essential for the activation of downstream targets that control cellular differentiation and proliferation in cardiac and neural development.

Pathways

HAND2 is an important participant in both heart development and neural crest cell signaling. It integrates signals in the BMP (Bone Morphogenetic Protein) pathway and partners with proteins such as NKX2-5 and GATA4 which are critical for orchestrating cardiac gene expression. HAND2 also plays a significant role in the WNT signaling pathway contributing to the proper guidance and differentiation of neural crest derivatives.

HAND2 mutations or dysregulation have links to congenital heart defects and neural crest-related disorders. In congenital heart defects altered HAND2 function impacts the expression levels of NKX2-5 and GATA4 leading to improper heart formation. Additionally HAND2's involvement in neural crest cell development associates its dysregulation with diseases such as Hirschsprung's disease. Pathological changes in HAND2 activity potentially disrupt the normal neural crest migration patterns contributing to these developmental anomalies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential for cardiac morphogenesis, particularly for the formation of the right ventricle and of the aortic arch arteries. Required for vascular development and regulation of angiogenesis, possibly through a VEGF signaling pathway. Also plays an important role in limb development, particularly in the establishment of anterior-posterior polarization, acting as an upstream regulator of sonic hedgehog (SHH) induction in the limb bud. Is involved in the development of branchial arches, which give rise to unique structures in the head and neck. Binds DNA on E-box consensus sequence 5'-CANNTG-3'.

See full target information Hand2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-HAND2 antibody [EPR19451]

Immunocytochemistry - Alexa Fluor® 647 Anti-HAND2 antibody [EPR19451] (AB270111)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise