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AB207050

Alexa Fluor® 647 Anti-HDAC9 antibody [EPR5223]

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(1 Publication)

Rabbit Recombinant Monoclonal HDAC9 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

HDAC7, HDAC7B, HDRP, KIAA0744, MITR, HDAC9, Histone deacetylase 9, HD9, Histone deacetylase 7B, Histone deacetylase-related protein, MEF2-interacting transcription repressor MITR, HD7, HD7b

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-HDAC9 antibody [EPR5223] (AB207050)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-HDAC9 antibody [EPR5223] (AB207050)

ab207050 staining HDAC9 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207050 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5223

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Human

Applications

ICC/IF, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Proteins & Peptides

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min).</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HDAC9 also known as Histone Deacetylase 9 is a protein involved in removing acetyl groups from histone proteins leading to chromatin condensation and transcriptional repression. HDAC9 has a molecular weight of about 127 kDa. It expresses predominantly in the brain muscle tissue and immune cells. This protein belongs to the class IIa family of histone deacetylases and has close structural similarities to other class IIa HDACs like HDAC4 HDAC5 and HDAC7.
Biological function summary

HDAC9 plays a significant role in regulating gene expression by altering the acetylation status of histones. It participates in the formation of large multiprotein complexes that target specific gene promoters. HDAC9's activity impacts various cellular processes including cell cycle progression and differentiation. It is a part of the CoREST complex which interacts with REST corepressor 1 to modulate neuronal gene expression and synaptic plasticity.

Pathways

HDAC9 is actively involved in key cellular signaling cascades. It contributes to the Notch signaling pathway which has a profound impact on cell differentiation and fate decisions. It also plays a role in the NF-kB pathway which is critical for immune response and inflammation. Through these pathways HDAC9 interacts with proteins such as RBP-Jk in the Notch pathway and IKK complex members in the NF-kB pathway indicating its broad influence on cellular mechanisms.

HDAC9 associates with several pathological conditions. It has been linked to cardiovascular diseases such as cardiac hypertrophy and heart failure due to its influence on cardiac muscle gene expression. HDAC9 also relates to various cancers including colon cancer where its dysregulation affects tumorigenesis and cell proliferation. In these contexts HDAC9 interacts with proteins like MEF2 and p300 which are pivotal in cardiac growth and cancer progression respectively.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.. Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.
See full target information HDAC9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of gastroenterology 56:673-687 PubMed34128109

2021

Essential role of Notch/Hes1 signaling in postnatal pancreatic exocrine development.

Applications

Unspecified application

Species

Unspecified reactive species

Katsutoshi Kuriyama,Yuzo Kodama,Masahiro Shiokawa,Yoshihiro Nishikawa,Saiko Marui,Takeshi Kuwada,Yuko Sogabe,Nobuyuki Kakiuchi,Teruko Tomono,Tomoaki Matsumori,Atsushi Mima,Toshihiro Morita,Tatsuki Ueda,Motoyuki Tsuda,Yuki Yamauchi,Yojiro Sakuma,Yuji Ota,Takahisa Maruno,Norimitsu Uza,Ryoichiro Kageyama,Tsutomu Chiba,Hiroshi Seno
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com