AB215919

Alexa Fluor® 647 Anti-Hemoglobin subunit alpha antibody [EPR3608]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal Hemoglobin subunit alpha antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

HBA2, HBA1, Hemoglobin subunit alpha, Alpha-globin, Hemoglobin alpha chain

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Hemoglobin subunit alpha antibody [EPR3608] (AB215919)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Hemoglobin subunit alpha antibody [EPR3608] (AB215919)

IHC image of Hemoglobin subunit alpha staining in a section of formalin-fixed paraffin-embedded normal human placenta*.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab215919 at 1/100 dilution (shown in red) and counterstained using ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Biotin

Biotin Anti-Hemoglobin subunit alpha antibody [EPR3608]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Hemoglobin subunit alpha antibody [EPR3608]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Hemoglobin subunit alpha antibody [EPR3608]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Hemoglobin subunit alpha antibody [EPR3608]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Hemoglobin subunit alpha antibody [EPR3608]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Hemoglobin subunit alpha antibody [EPR3608]
Unconjugated

Anti-Hemoglobin subunit alpha antibody [EPR3608]
Carrier free

Anti-Hemoglobin subunit alpha antibody [EPR3608] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3608

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hemoglobin subunit alpha also known as alpha-globin is a component of the hemoglobin protein complex which plays an important role in oxygen transport within the blood. Alpha-globin has an approximate molecular weight of 15.1 kDa and is expressed highly in the red blood cells. It is part of the hemoglobin tetramer along with two beta subunits each one containing an iron-bound heme group. Variants of the alpha chain can be studied using hemoglobin peptides or denatured hemoglobin samples. Researchers can further analyze the alpha hemoglobin using methods like SDS-PAGE or alpha ELISA assays.

Biological function summary

Hemoglobin subunit alpha forms an important part of the hemoglobin complex facilitating the binding and release of oxygen molecules as blood circulates in the body. Alpha hemoglobin ensures efficient loading of oxygen in the lungs and unloading in tissues maintaining cellular respiration. The subunit plays a structural role as well stabilizing the hemoglobin tetramer for optimal function. Its ability to carry oxygen depends on the cooperative interaction between its alpha and beta globin counterparts.

Pathways

Hemoglobin subunit alpha operates predominantly within the oxygen transport pathway which is essential to meet the metabolic demands of cells. It also links to pathways involving iron metabolism given its coordination with heme groups. Alpha hemoglobin interacts cooperatively with proteins such as beta-globin to ensure efficient oxygen delivery. This interplay is highlighted when examining hemoglobin biosynthesis and breakdown pathways.

Alpha-globin is linked to conditions like alpha-thalassemia and sickle cell disease. These disorders result from mutations in the hemoglobin alpha or beta subunits leading to imbalanced globin production or abnormal hemoglobin structures. Alpha thalassemia is connected with unequal production of globin chains affecting hemoglobin stability while beta-globin mutations lead to sickle cell disease with altered oxygen delivery. Anti-hemoglobin antibodies might help in researching these conditions allowing a better understanding of molecular changes and potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in oxygen transport from the lung to the various peripheral tissues.. Hemopressin. Hemopressin acts as an antagonist peptide of the cannabinoid receptor CNR1 (PubMed : 18077343). Hemopressin-binding efficiently blocks cannabinoid receptor CNR1 and subsequent signaling (PubMed : 18077343).

See full target information HBA1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 10: PubMed40627458

2025

Tumor microenvironments with an active type I IFN response are sensitive to inhibitors of heme degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Dominika Sosnowska,Tik Shing Cheung,Jit Sarkar,James W Opzoomer,Karen T Feehan,Joanne E Anstee,Chloé A Woodman,Mohamed Reda Keddar,Kalum Clayton,Samira Ali,William Macmorland,Dorothy D Yang,James Rosekilly,Cheryl E Gillett,Francesca D Ciccarelli,Richard Buus,James Spicer,Anita Grigoriadis,James N Arnold

Circulation 151:8-30 PubMed39633569

2024

Sickle Trait and Alpha Thalassemia Increase NOS-Dependent Vasodilation of Human Arteries Through Disruption of Endothelial Hemoglobin-eNOS Interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Steven D Brooks,A Parker Ruhl,Xianke Zeng,Phillip Cruz,Sergio A Hassan,Olena Kamenyeva,Md Abdul Hakim,Lauryn A Ridley,Bianca M Nagata,Juraj Kabat,Sundar Ganesan,Rachel L Smith,Mary Jackson,Jessica Nino de Rivera,Alison J McLure,Jarrett M Jackson,Robert O Emeh,Naomi Tesfuzigta,Kyeisha Laurence,Stacy Joyce,Christina Yek,Sophana Chea,Derron A Alves,Brant E Isakson,Jessica Manning,Jeremy L Davis,Hans C Ackerman

Journal of cellular and molecular medicine 24:6162-6177 PubMed32351032

2020

Smad2/3-pathway ligand trap luspatercept enhances erythroid differentiation in murine β-thalassaemia by increasing GATA-1 availability.

Applications

Unspecified application

Species

Unspecified reactive species

Pedro A Martinez,Robert Li,Harish N Ramanathan,Manoj Bhasin,R Scott Pearsall,Ravindra Kumar,Rajasekhar N V S Suragani

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-Hemoglobin subunit alpha antibody [EPR3608]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Hemoglobin subunit alpha antibody [EPR3608] (AB215919)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise