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AB315258

Alexa Fluor® 647 Anti-Hepcidin + Hepcidin-2 antibody [EPR18937]

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Rabbit Recombinant Monoclonal HEPC2 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Mouse samples.

View Alternative Names

Hepc2, Hamp2, Hepcidin-2, Hamp1, Hepc, Hepc1, Hamp, Hepcidin

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (AB315258)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (AB315258)

Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling Hepcidin + Hepcidin-2 with ab315258 at 1/50 (10.0 ug/ml).

Negative control : no staining on mouse brain. The section was incubated with ab315258 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (AB315258)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (AB315258)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Hepcidin + Hepcidin-2 with ab315258 at 1/50 (10.0 ug/ml).

Cytoplasmic staining on mouse liver. The section was incubated with ab315258 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

  • Unconjugated

    Anti-Hepcidin + Hepcidin-2 antibody [EPR18937]

  • Carrier free

    Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18937

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Mouse, Mouse

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

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Proteins & Peptides

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Proteins & Peptides

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hepcidin also known as LEAP-1 and Hepcidin-2 are small peptides with Hepcidin having a mass of approximately 2.8 kDa. These peptides synthesize in the liver and release into the circulatory system. Hepcidins regulate iron homeostasis by binding to the iron exporter ferroportin which is present on the surface of enterocytes macrophages and hepatocytes. This binding causes ferroportin internalization and degradation reducing iron release into the bloodstream.
Biological function summary

The iron-regulating proteins are key regulators of systemic iron metabolism. Hepcidin acts as a hormone controlling the distribution of iron a vital element for many physiological processes. Hepcidin-2 serves a similar role but its specific role is less defined compared to Hepcidin. Though they don't form complexes with other proteins their activity is critical for maintaining controlled levels of iron throughout the body preventing both deficiency and overload.

Pathways

Hepcidin and Hepcidin-2 are integral within the iron and hepcidin regulatory pathway which coordinates body iron levels. They play roles in the bone morphogenetic protein (BMP) signaling pathway. The pathway involves several other proteins like BMP6 and SMAD4 which influence hepcidin expression in response to iron levels and the body's erythropoietic activity.

Hepcidin regulation has implications in conditions like hereditary hemochromatosis and anemia of inflammation. Hereditary hemochromatosis results from insufficient Hepcidin activity leading to iron overload. Conversely anemia of inflammation shows increased Hepcidin levels reducing iron availability and contributing to anemia. Hepcidin dysregulation connects to other proteins such as HFE and transferrin receptor 2 which play roles in iron overload disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Seems to act as a signaling molecule involved in the maintenance of iron homeostasis.
See full target information Hamp2

Additional targets

Hamp
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com