JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB237314

Alexa Fluor® 647 Anti-Hexokinase II antibody [EPR20839]

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Recombinant Monoclonal Hexokinase II antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

Hexokinase-2, Hexokinase type II, Hexokinase-B, Muscle form hexokinase, HK II, HK2

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Hexokinase II antibody [EPR20839] (AB237314)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Hexokinase II antibody [EPR20839] (AB237314)

ab237314 staining Hexokinase II in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab237314 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • HRP

    HRP Anti-Hexokinase II antibody [EPR20839]

  • Unconjugated

    Anti-Hexokinase II antibody [EPR20839]

  • Carrier free

    Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20839

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Not recommended for rat and mouse tissues in IHC.

style
left-column

Complementary Products

Assay Kits

AB136957

Hexokinase Activity Assay Kit (Colorimetric)

Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (AB136957)

  • 0
  • 0
Primary Antibodies

AB171449

Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9]

KO Validated

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (AB171449)

  • 5
  • 4
Primary Antibodies

AB195020

Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB195020)

  • 4
  • 5
Primary Antibodies

AB202652

Alexa Fluor® 488 Anti-Lactate Dehydrogenase antibody [EP1566Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lactate Dehydrogenase antibody [EP1566Y] (AB202652)

  • 3
  • 1
Primary Antibodies

AB212122

PE Anti-IDH2 antibody [EPR7577]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - PE Anti-IDH2 antibody [EPR7577] (AB212122)

  • 5
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively) (PubMed : 23185017, PubMed : 26985301, PubMed : 29298880). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed : 29298880). Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis (PubMed : 18350175).
See full target information HK2
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:111279 PubMed39628557

2024

Dietary caloric restriction protects experimental autoimmune uveitis by regulating Teff/Treg balance.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaohuai Li,Runping Duan,Qi Jiang,Jiaying Liu,Jialing Chen,Loujing Jiang,Tianfu Wang,He Li,Yihan Zhang,Xuening Peng,Zhaohao Huang,Lei Zhu,Wenjun Zou,Ying Lin,Wenru Su

Cell reports. Medicine :100978 PubMed36933554

2023

mRNAs encoding IL-12 and a decoy-resistant variant of IL-18 synergize to engineer T cells for efficacious intratumoral adoptive immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Irene Olivera,Elixabet Bolaños,Jose Gonzalez-Gomariz,Sandra Hervas-Stubbs,Karina V Mariño,Carlos Luri-Rey,Iñaki Etxeberria,Assunta Cirella,Josune Egea,Javier Glez-Vaz,Saray Garasa,Maite Alvarez,Iñaki Eguren-Santamaria,Sonia Guedan,Miguel F Sanmamed,Pedro Berraondo,Gabriel A Rabinovich,Alvaro Teijeira,Ignacio Melero

Nature communications 13:6458 PubMed36309526

2022

Single-cell profiling reveals distinct adaptive immune hallmarks in MDA5+ dermatomyositis with therapeutic implications.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Ye,Zechuan Chen,Shan Jiang,Fengyun Jia,Teng Li,Xia Lu,Jing Xue,Xinyue Lian,Jiaqiang Ma,Pei Hao,Liangjing Lu,Shuang Ye,Nan Shen,Chunde Bao,Qiong Fu,Xiaoming Zhang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com