AB245912

Alexa Fluor® 647 Anti-Histone H3 (acetyl K27) antibody [EP16602]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal H3 acetyl K27 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3FC, HIST1H3C, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K27ac

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245912)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245912)

Immunofluorescent analysis of 4% PFA-fixed, 0.1% Triton X-100 permeabilized HEK-293T HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Histone H3 with ab245912 at 1/200 dilution (red). Confocal image showing increased nuclear staining in HeLa cells treated with TSA (500ng/ml 4h). Counterstained with ab195887 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 488). Nuclear counterstain is DAPI (blue).

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Histone H3 (acetyl K27) antibody [EP16602]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade
660 APC

APC Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade
578 PE

PE Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade
Unconjugated

Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade
Carrier free

Anti-Histone H3 (acetyl K27) antibody [EP16602] – Ms IgG1 (Chimeric) – BSA and Azide Free
Carrier free

Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP16602

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.

See full target information H3C1 acetyl K27

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell 186:363-381.e19 PubMed36669472

2023

Multiplexed 3D atlas of state transitions and immune interaction in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Ren Lin,Shu Wang,Shannon Coy,Yu-An Chen,Clarence Yapp,Madison Tyler,Maulik K Nariya,Cody N Heiser,Ken S Lau,Sandro Santagata,Peter K Sorger

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-Histone H3 (acetyl K27) antibody [EP16602]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Histone H3 (acetyl K27) antibody [EP16602] (AB245912)

Related conjugates and formulations (9)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

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Product promise