Rabbit Recombinant Monoclonal H3 mutated K36M antibody - conjugated to Alexa Fluor® 647. Suitable for ICC and reacts with Synthetic peptide - Human samples.
Images
![Immunocytochemistry - Alexa Fluor® 647 Anti-Histone H3 (mutated K36M) antibody [EPR23614-91] (AB275178), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-histone-h3-mutated-k36m-antibody-epr23614-91-ab275178--immunocytochemistry-img81521.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Alexa Fluor® 647 Anti-Histone H3 (mutated K36M) antibody [EPR23614-91] (AB275178)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Alexa Fluor® 647
- Excitation/Emission
Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC | |
|---|---|
| Human | Predicted |
| Synthetic peptide - Human | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes This product gave a positive signal in HEK-293 cell line transfected with myc-tagged Histone H3.3 K36M fixed with 4% formaldehyde (10 min). |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
H3.3A, H3F3, H3F3A, PP781, H3-3B, H3.3B, H3F3B, H3.3B, H3F3B, H3-3B, PP781, H3F3A, H3F3, H3.3A, H3-3A, Histone H3.3
Recommended products
Rabbit Recombinant Monoclonal H3 mutated K36M antibody - conjugated to Alexa Fluor® 647. Suitable for ICC and reacts with Synthetic peptide - Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Alexa Fluor® 647
- Excitation/Emission
Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR23614-91
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunocytochemistry - Alexa Fluor® 647 Anti-Histone H3 (mutated K36M) antibody [EPR23614-91] (ab275178), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-histone-h3-mutated-k36m-antibody-epr23614-91-ab275178--immunocytochemistry-img81521.jpg "Immunocytochemistry - Alexa Fluor® 647 Anti-Histone H3 (mutated K36M) antibody [EPR23614-91] (ab275178)")
Immunocytochemistry - Alexa Fluor® 647 Anti-Histone H3 (mutated K36M) antibody [EPR23614-91] (ab275178)
ab275178 staining Histone H3.3K36M in HEK-293T cells transfected with a Myc tagged human Histone H3.3 construct, with negative expression in Myc transfected HEK-293T cells.
The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab275178 at 1/500 dilution (shown in red) and Myc-Tag Mouse mAb (Alexa Fluor® 488) at 1/100 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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