AB196698

Alexa Fluor® 647 Anti-Histone H3 (phospho S10) antibody [mAbcam 14955]

Name: Anti-Histone H3 (phospho S10) Alexa Fluor® 647 antibody [mAbcam 14955] (ab196698) | Abcam
Brand: Abcam Limited
SKU: AB196698
Price: 575 USD
Availability: InStock

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(5 Publications)

Mouse Monoclonal H3 phospho S10 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 5 publications.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3S10p

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB196698)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB196698)

ab196698 staining Histone H3 (phospho S10) in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1 %PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196698 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Histone H3 (phospho S10) antibody [mAbcam 14955]
Unconjugated

Anti-Histone H3 (phospho S10) antibody [mAbcam 14955]
Carrier free

Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

mAbcam 14955

Isotype

IgG1

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Arabidopsis thaliana": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Aspergillus nidulans": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Caenorhabditis elegans": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chlamydomonas reinhardtii": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Indian muntjac": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mammals": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Neurospora crassa": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Saccharomyces cerevisiae": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Schizosaccharomyces pombe": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Tobacco": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.

See full target information H3C1 phospho S10

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7930 PubMed40855028

2025

Dual mitotic bookmarking by GAF and H3K27ac orchestrates differential propagation of cell fate memory in neural development.

Applications

Unspecified application

Species

Unspecified reactive species

Rulan Zhang,Jie Liu,Zimo Zhang,Zili Chen,Tanpeng Wang,Yuying Shen,Zejun Lan,Jingyi Chu,Haoxuan Tang,Xiyue Zhang,Yan Song

STAR protocols 6:103858 PubMed40450696

2025

Protocol for identifying genomic binding sites of mitotic bookmarkers in Drosophila neural stem cells and cultured mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuying Shen,Jie Liu,Yan Song

Cell reports 43:113889 PubMed38416646

2024

Two transcriptional cascades orchestrate cockroach leg regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Chonghua Ren,Yejie Wen,Shaojuan Zheng,Zheng Zhao,Ethan Yihao Li,Chenjing Zhao,Mingtao Liao,Liang Li,Xiaoshuai Zhang,Suning Liu,Dongwei Yuan,Kai Luo,Wei Wang,Jifeng Fei,Sheng Li

Nature genetics 55:232-245 PubMed36658434

2023

Notch1 mutations drive clonal expansion in normal esophageal epithelium but impair tumor growth.

Applications

Unspecified application

Species

Unspecified reactive species

Emilie Abby,Stefan C Dentro,Michael W J Hall,Joanna C Fowler,Swee Hoe Ong,Roshan Sood,Albert Herms,Gabriel Piedrafita,Irina Abnizova,Christian W Siebel,Moritz Gerstung,Benjamin A Hall,Philip H Jones

International journal of molecular sciences 22: PubMed34830164

2021

Nitric Oxide-Dependent Mechanisms Underlying MK-801- or Scopolamine-Induced Memory Dysfunction in Animals: Mechanistic Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Paulina Cieślik,Anna Siekierzycka,Adrianna Radulska,Agata Płoska,Grzegorz Burnat,Piotr Brański,Leszek Kalinowski,Joanna M Wierońska

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-Histone H3 (phospho S10) antibody [mAbcam 14955]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB196698)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

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