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AB223996

Alexa Fluor® 647 Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606]

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(1 Publication)

Rabbit Recombinant Monoclonal Histone H4 acetyl K8 + K12 + K16 + K5 + K8 + K12 + K16 + K5 + K8 + K12 + K16 + K8 + K12 + K16 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4C1, Histone H4

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (AB223996)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (AB223996)

ab223996 staining Histone H4 (acetyl K5 + K8 + K12 + K16) in HeLa cells +/- Trichostatin A (500ng/ml, 4 hours). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h.

The cells were then incubated overnight at +4°C with ab223996 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606]

  • 660 APC

    APC Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606]

  • 578 PE

    PE Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16606

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells treated with Trichostatin A (500ng/ml, 4 hours) fixed with 4% formaldehyde (10 min)</p>" } } }

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information H4C1 acetyl K8 + K12 + K16 + K5 + K8 + K12 + K16 + K5 + K8 + K12 + K16 + K8 + K12 + K16

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 27:1482-1495 PubMed40921734

2025

Hbo1 and Msl complexes preserve differential compaction and H3K27me3 marking of active and inactive X chromosomes during mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dounia Djeghloul,Sherry Cheriyamkunnel,Bhavik Patel,Holger Kramer,Alex Montoya,Karen E Brown,Chad Whilding,Tatyana B Nesterova,Guifeng Wei,Neil Brockdorff,Iga Grządzielewska,Remzi Karayol,Asifa Akhtar,Matthias Merkenschlager,Amanda G Fisher
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com