Alexa Fluor® 647 Anti-hnRNP A2B1 antibody [EPR24002-81]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-hnRNP A2B1 antibody [EPR24002-81] (AB300734)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling hnRNP A2B1 with ab300734 at 1/100 (5.0 µg/ml). Nuclear staining on human colon is observed. The section was incubated with ab300734 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-hnRNP A2B1 antibody [EPR24002-81] (AB300734)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling hnRNP A2B1 with ab300734 at 1/50 dilution (10.0 µg/ml) (Red). Confocal image showing nuclear staining in HeLa cell line. ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Green). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-hnRNP A2B1 antibody [EPR24002-81] (AB300734)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HNRNPA2B1 wild-type and KO HEK293T (ab266404) cells labelling hnRNP A2B1 with ab300734 at 1/50 dilution (10.0 µg/ml) (Red). Confocal image showing nuclear staining in 293T cells and nearly no staining in HNRNPA2B1 knockout 293T cells. ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Green). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-hnRNP A2B1 antibody [EPR24002-81] (AB300734)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling hnRNP A2B1 with ab300734 at 1/50 (10.0 ug/ml) msd2577,(Green). Confocal image showing nuclear staining in NIH/3T3 cell line is observed. is observed. ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml msd2577 (Red). The Nuclear counterstain was DAPI (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-hnRNP A2B1 antibody [EPR24002-81] (AB300734)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling hnRNP A2B1 with ab300734 at 1/100 (5.0 µg/ml). Nuclear staining on rat colon is observed. The section was incubated with ab300734 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval with performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins.