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Rabbit Recombinant Monoclonal HNRPAB antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-HNRPAB antibody [EPR16944] (AB210053), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested
Mouse
Predicted
Rat
Predicted

Tested
Tested

Species
Human
Dilution info
1/100
Notes

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Binds single-stranded RNA. Has a high affinity for G-rich and U-rich regions of hnRNA. Also binds to APOB mRNA transcripts around the RNA editing site.

Alternative names

ABBP1, HNRPAB, HNRNPAB, Heterogeneous nuclear ribonucleoprotein A/B, hnRNP A/B, APOBEC1-binding protein 1, ABBP-1

Recommended products

Rabbit Recombinant Monoclonal HNRPAB antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16944
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Heterogeneous nuclear ribonucleoprotein A/B often called HNRPAB functions as a part of the ribonucleoprotein complex. This protein interacts with pre-mRNA in the nucleus and influences its processing. HNRPAB has a molecular mass of about 37 kilodaltons. Scientists find it highly expressed in various tissues including the brain and testis indicating its significant role in these areas. The protein contains RNA-binding domains and plays a part in the regulation of alternative splicing.

Biological function summary

HNRPAB influences the splicing and transport of pre-mRNA impacting the maturation process of mRNA. It collaborates with other hnRNPs and splicing factors to modulate gene expression. The protein sometimes forms part of larger complexes that are essential for RNA processing. This complex helps to stabilize RNA molecules assisting in their proper transport and translation in the cytoplasm.

Pathways

HNRPAB is involved in the regulation of RNA metabolism and processing pathways. It forms a network with other proteins like hnRNP F and hnRNP H impacting gene expression by influencing mRNA stability and availability. In conjunction with these proteins HNRPAB plays a role in the signal transduction pathways affecting cellular responses and adaptations.

Associated diseases and disorders

HNRPAB has connections to cancer and neurodegenerative disorders. Its dysregulation can lead to abnormal splicing patterns contributing to oncogenesis. In cancer associations with proteins like c-Myc indicate a role in tumorigenesis. In neurodegenerative diseases altered HNRPAB activity could impact neuronal survival contributing to the progression of disorders like Alzheimer’s disease. The protein’s interaction with tau suggests a potential link to tauopathies which are related to abnormal protein aggregations within neurons.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-HNRPAB antibody [EPR16944] (ab210053), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-HNRPAB antibody [EPR16944] (ab210053)

    ab210053 staining HNRPAB in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210053 at 1/100 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com