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AB196716

Alexa Fluor® 647 Anti-HOXB13 antibody [mAbcam53931]

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(1 Publication)

Mouse Monoclonal HOXB13 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Homeobox protein Hox-B13, HOXB13

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-HOXB13 antibody [mAbcam53931] (AB196716)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-HOXB13 antibody [mAbcam53931] (AB196716)

ab196716 staining HOXB13 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196716 at a 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

mAbcam53931

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HOXB13 also known as Homeobox protein Hox-B13 is a transcription factor with an important role in gene regulation. It weighs around 30 kDa. HOXB13 is part of the homeobox gene family which means it contains a DNA-binding domain known as the homeodomain. HOXB13 primarily expresses in the prostate skin and parts of the intestine. In these tissues it regulates specific genes that drive cellular development and differentiation processes.
Biological function summary

HOXB13 guides tissue patterning and morphological development. It functions within a group of proteins forming transcriptional regulatory complexes. This protein influences cellular differentiation especially in the prostate and plays a part in epidermal development. HOXB13's regulatory functions extend to several genes that define cell fate and organogenesis contributing significantly to tissue-specific function.

Pathways

HOXB13 interacts heavily within the Wnt signaling pathway which is key for cell proliferation and differentiation. It also interfaces with the androgen receptor signaling pathway. HOXB13 modulates genes influenced by androgen signaling thereby impacting cell growth and differentiation in the prostate. Moreover its interaction with proteins such as β-catenin within the Wnt pathway highlights its involvement in cell development and communication.

HOXB13 has associations with prostate cancer and colorectal cancer. Mutations or dysregulation of HOXB13 can alter normal cellular behavior leading to malignancies in these tissues. In prostate cancer HOXB13 can interact with other proteins like the androgen receptor that drive tumor progression through aberrant signaling. In colorectal cancer HOXB13's role in gene regulation can lead to disrupted cellular homeostasis when mutated contributing to tumorigenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds preferentially to methylated DNA (PubMed : 28473536).
See full target information HOXB13
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Genes 11: PubMed32585969

2020

Leukocyte Nucleolus and -When Falling Apart Means Falling in Place.

Applications

Unspecified application

Species

Unspecified reactive species

Ivona Mladineo,Jerko Hrabar
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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