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AB307614

Alexa Fluor® 647 Anti-ICAM1 antibody [EPR22161-284]

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Rabbit Recombinant Monoclonal ICAM1 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt, ICC/IF and reacts with Mouse samples.

View Alternative Names

CD54, Icam-1, Icam1, Intercellular adhesion molecule 1, ICAM-1, MALA-2, MyD10

3 Images
Flow Cytometry - Alexa Fluor® 647 Anti-ICAM1 antibody [EPR22161-284] (AB307614)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Anti-ICAM1 antibody [EPR22161-284] (AB307614)

Flow cytometric analysis of Mouse splenocytes cells labelling ICAM1 with ab307614 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Gated on viable cells.

Flow Cytometry - Alexa Fluor® 647 Anti-ICAM1 antibody [EPR22161-284] (AB307614)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Anti-ICAM1 antibody [EPR22161-284] (AB307614)

Flow cytometric analysis of L929 (mouse connective tissue fibroblast) cells labelling ICAM1 with ab307614 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Gated on viable cells. Negative control : L929 (PMID : 35056985).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ICAM1 antibody [EPR22161-284] (AB307614)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ICAM1 antibody [EPR22161-284] (AB307614)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A20 (mouse reticulum sarcoma B lymphocyte) cells labelling ICAM1 with ab307614 at 1/50 (10.0 µg/ml) dilution. Confocal image showing membranous staining in A20 cells. Negative control : L-929 (PMID : 35056985). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22161-284

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Mouse

Applications

ICC/IF, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).
See full target information Icam1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Version
Primary Antibodies

AB239401

Anti-ICAM1 antibody [EPR22161-284] - BSA and Azide free

primary-antibodies

icam1-antibody-epr22161-284-bsa-and-azide-free-ab239401

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Alternative Version
Primary Antibodies

AB302655

Alexa Fluor® 488 Anti-ICAM1 antibody [EPR22161-284]

primary-antibodies

alexa-fluor-488-icam1-antibody-epr22161-284-ab302655

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(0 reviews)

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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