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Rabbit Recombinant Monoclonal ICAM1 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt, ICC/IF and reacts with Mouse samples.

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ICAM1 antibody [EPR22161-284] (AB307614), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 647 Anti-ICAM1 antibody [EPR22161-284] (AB307614), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 647 Anti-ICAM1 antibody [EPR22161-284] (AB307614), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICC/IF
Mouse
Tested
Tested

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

Target data

Function

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).

Alternative names

Intercellular adhesion molecule 1, ICAM-1, MALA-2, MyD10, Icam-1, Icam1

Recommended products

Rabbit Recombinant Monoclonal ICAM1 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt, ICC/IF and reacts with Mouse samples.

Alternative names

Intercellular adhesion molecule 1, ICAM-1, MALA-2, MyD10, Icam-1, Icam1

Key facts

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22161-284

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ICAM1 antibody [EPR22161-284] (ab307614), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ICAM1 antibody [EPR22161-284] (ab307614)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A20 (mouse reticulum sarcoma B lymphocyte) cells labelling ICAM1 with ab307614 at 1/50 (10.0 µg/ml) dilution. Confocal image showing membranous staining in A20 cells.

    Negative control: L-929 (PMID:35056985). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

  • Flow Cytometry - Alexa Fluor® 647 Anti-ICAM1 antibody [EPR22161-284] (ab307614), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 647 Anti-ICAM1 antibody [EPR22161-284] (ab307614)

    Flow cytometric analysis of Mouse splenocytes cells labelling ICAM1 with ab307614 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 647 Anti-ICAM1 antibody [EPR22161-284] (ab307614), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 647 Anti-ICAM1 antibody [EPR22161-284] (ab307614)

    Flow cytometric analysis of L929 (mouse connective tissue fibroblast) cells labelling ICAM1 with ab307614 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Gated on viable cells. Negative control: L929 (PMID: 35056985).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com