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Rabbit Recombinant Monoclonal ILT-4 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.

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Images

Flow Cytometry - Alexa Fluor® 647 Anti-ILT-4 antibody [EPR22081] (AB313898), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ILT-4 antibody [EPR22081] (AB313898), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow Cyt
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

Target data

Function

Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles (PubMed:11169396, PubMed:12853576, PubMed:16455647, PubMed:20448110, PubMed:27859042). Involved in the down-regulation of the immune response and the development of tolerance. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (peptide-bound HLA-G-B2M) triggering differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance (PubMed:16455647, PubMed:20448110, PubMed:27859042). Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions (PubMed:11875462, PubMed:12853576, PubMed:9548455, PubMed:9842885).

Alternative names

CD85d, ILT4, LIR2, MIR10, LILRB2, Leukocyte immunoglobulin-like receptor subfamily B member 2, LIR-2, Leukocyte immunoglobulin-like receptor 2, CD85 antigen-like family member D, Immunoglobulin-like transcript 4, Monocyte/macrophage immunoglobulin-like receptor 10, ILT-4, MIR-10

Recommended products

Rabbit Recombinant Monoclonal ILT-4 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22081
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ILT-4 also known as Immunoglobulin-like transcript 4 is a transmembrane protein with a molecular weight of approximately 45 kDa. The protein is part of the LILR family and is sometimes referred to as LILRB2 protein. ILT-4 is mainly expressed on myeloid lineage cells including monocytes and dendritic cells. The expression of ILT-4 is important for the modulation of the immune response. Biotinylated ILT-4 and biotinylated LILRB2 versions are used frequently in research to study their interactions and functions.

Biological function summary

ILT-4 plays an important role in the regulation of immune tolerance and inhibition. It is not part of a complex but interacts with other immune regulatory proteins. By binding to MHC class I molecules ILT-4 inhibits the activation of immune responses preventing over-activation and autoimmunity. The ILT-4 protein is critical for helping myeloid cells to maintain a balanced immune response often restricting unnecessary inflammation and promoting tolerance.

Pathways

ILT-4 influences immune signaling pathways such as those that control T cell receptor signaling. ILT-4 interactions with proteins like CD85d and other LILRA6 proteins showcase its involvement in negative regulatory signaling pathways. These pathways are essential for maintaining immune homeostasis and limiting immune-mediated damage during infections or tissue injury.

Associated diseases and disorders

ILT-4 is implicated in cancer and autoimmune diseases such as multiple sclerosis. In cancers ILT-4 modulates the immune environment allowing tumors to evade immune surveillance. In the context of autoimmune disorders aberrant ILT-4 expression or function can lead to insufficient immune regulation contributing to disease progression. CD85d and LILRB2 proteins are also associated with these conditions further influencing ILT-4's role in disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry - Alexa Fluor® 647 Anti-ILT-4 antibody [EPR22081] (ab313898), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 647 Anti-ILT-4 antibody [EPR22081] (ab313898)

    Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) cells labelling ILT-4 with ab313898 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Cells are co-stained with CD14 conjugated to BV510. Gated on viable cells.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ILT-4 antibody [EPR22081] (ab313898), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ILT-4 antibody [EPR22081] (ab313898)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human PBMC (human peripheral blood mononuclear cell) cells labelling ILT-4 with ab313898 at 1/50 (10.0 ug/ml) dilution (Red). Confocal image showing membranous staining in subsets of Human PBMC. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Green). The Nuclear counterstain was DAPI (Blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com