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Rabbit Recombinant Monoclonal IMPDH2 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-IMPDH2 antibody [EPR8364(B)] (AB200775), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/250
Notes

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min).

Associated Products

Select an associated product type

2 products for Alternative Version

1 product for Alternative Product

Target data

Function

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth (PubMed:7763314, PubMed:7903306). Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism (PubMed:14766016). It may also have a role in the development of malignancy and the growth progression of some tumors.

Alternative names

IMPD2, IMPDH2, Inosine-5'-monophosphate dehydrogenase 2, IMP dehydrogenase 2, IMPD 2, IMPDH 2, Inosine-5'-monophosphate dehydrogenase type II, IMP dehydrogenase II, IMPDH-II

Recommended products

Rabbit Recombinant Monoclonal IMPDH2 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR8364(B)
Purification technique
Affinity purification Protein A
Dissociation constant
2.2 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

IMPDH2 also known as inosine monophosphate dehydrogenase 2 plays an important role in the de novo synthesis of guanine nucleotides. This enzyme with a molecular mass of approximately 56 kDa catalyzes the conversion of inosine monophosphate (IMP) to xanthosine monophosphate (XMP) an important step in the nucleotide biosynthesis. IMPDH2 is expressed in various tissues across the body with high expression in proliferating cells due to increased nucleotide requirements.

Biological function summary

IMPDH2 functions as a homotetramer forming part of a larger complex that regulates nucleotide levels. This regulation is critical for cell growth differentiation and proliferation. IMPDH2 activity drives the synthesis of guanine nucleotides which are essential for DNA and RNA synthesis influencing cellular communication and energy transfer.

Pathways

The target IMPDH2 has a significant role in the purine nucleotide biosynthesis pathway aligning with other enzymes like GMP synthetase. This pathway is critical in maintaining cellular nucleotide pools. IMPDH2’s activity closely interacts with guanosine monophosphate reductase within the same pathway ensuring the balance between different types of nucleotides necessary for various cellular processes.

Associated diseases and disorders

Aberrations in IMPDH2 activity link to immunological diseases and cancer. Overexpression or upregulation of this enzyme contributes to the rapid proliferation seen in cancerous cells by maintaining high guanine nucleotide levels. Additionally IMPDH2 serves as a target for immunosuppressive drugs used in autoimmune conditions highlighting its relationship with T- and B-cell proliferation. This positions IMPDH2 in therapeutic strategies alongside its pathway partners to modulate disease outcomes.

Product promise

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1 product image

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-IMPDH2 antibody [EPR8364(B)] (ab200775), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-IMPDH2 antibody [EPR8364(B)] (ab200775)

    ab200775 staining IMPDH2 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200775 at a 1/250 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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