AB209027

Alexa Fluor® 647 Anti-iNOS antibody [EPR16635]

RabMAb
Recombinant
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(14 Publications)

Rabbit Recombinant Monoclonal iNOS antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 14 publications.

View Alternative Names

NOS2A, NOS2, Hepatocyte NOS, Inducible NO synthase, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, HEP-NOS, Inducible NOS, iNOS

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-iNOS antibody [EPR16635] (AB209027)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-iNOS antibody [EPR16635] (AB209027)

ab209027 staining iNOS in Raw264.7 cells. The lower panel shows cells treated with 1ug/ml Lipopolysaccharides (24 hr). The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209027at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in SW480 cells fixed with 4% formaldehyde (10 min).

Carrier free

Anti-iNOS antibody [EPR16635] - BSA and Azide free
Unconjugated

Anti-iNOS antibody [EPR16635]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-iNOS antibody [EPR16635]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-iNOS antibody [EPR16635]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16635

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Mouse, Human, Mouse, Rat

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "This product gave a positive signal in LPS-treated Raw264.7 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)." }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Inducible Nitric Oxide Synthase commonly referred to as iNOS or NOS2 is an important enzyme in the production of nitric oxide (NO) from L-arginine. This enzyme has a molecular weight of approximately 130 kDa and is expressed in various cell types including macrophages and hepatocytes. iNOS becomes active in response to inflammatory stimuli and can produce large amounts of NO for extended periods. Western blotting and iNOS ELISA kits are frequently used methods to detect and quantify the presence and activity of iNOS in different biological samples.

Biological function summary

INOS plays a significant role in the immune system by participating in the defense against pathogens. The enzyme is part of a molecular complex that generates NO which acts as a signaling molecule and has antimicrobial properties. iNOS activity is induced during immune responses and contributes to the elimination of bacteria and viruses through the production of reactive nitrogen species. Its expression is tightly regulated by cytokines and other signaling molecules that modulate the extent of its action.

Pathways

INOS integrates into the nitric oxide signaling pathway and is closely associated with inflammatory pathways. NO serves as a messenger within the nitric oxide signaling pathway facilitating processes like vasodilation and neurotransmission. iNOS works alongside other proteins such as cytokines like interferon-gamma that elevate its activity during inflammation and immune responses. The regulation of iNOS expression and activity plays an essential role in maintaining immune homeostasis and ensuring proper response to infections.

INOS is linked to conditions characterized by excessive inflammation such as rheumatoid arthritis and sepsis. In these diseases overproduction of nitric oxide by iNOS can lead to tissue damage and exacerbate symptoms. Additionally iNOS is associated with the protein TNF-alpha as its expression is often driven by inflammatory mediators during disease states. Understanding the role of iNOS in such conditions provides insights into potential therapeutic targets for managing inflammatory diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed : 7504305, PubMed : 7531687, PubMed : 7544004, PubMed : 7682706). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed : 25417112). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (PubMed : 19688109).

See full target information NOS2

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 15:1466208 PubMed39717556

2024

Synergistic combinations of for myocardial infarction treatment: network pharmacology and quadratic optimization approach.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Di Wang,Xin-Yi Fan,Xiao-Qi Wei,Wang-Jing Chai,Fang-He Li,Kuo Gao,Bin Liu,Shu-Zhen Guo

Journal of nanobiotechnology 22:540 PubMed39237942

2024

Enhanced osteogenic differentiation in 3D hydrogel scaffold via macrophage mitochondrial transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Shui Qiu,Lili Cao,Dingding Xiang,Shu Wang,Di Wang,Yiyi Qian,Xiaohua Li,Xiaoshu Zhou

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2309725 PubMed38647360

2024

Lactobacillus Reuteri Vesicles Regulate Mitochondrial Function of Macrophages to Promote Mucosal and Cutaneous Wound Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Chen,Xiaoyao Huang,Anqi Liu,Siyuan Fan,Shiyu Liu,Zihan Li,Xiaoxue Yang,Hao Guo,Meiling Wu,Meng Liu,Peisheng Liu,Fei Fu,Siying Liu,Kun Xuan

Advanced healthcare materials 12:e2300028 PubMed36876892

2023

Targeting STING Activation by Antigen-Inspired MnO Nanovaccines Optimizes Tumor Radiotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Gu,Subin Lin,Yanxian Wu,Pei Xu,Wen Zhu,Yangyun Wang,Xiaju Cheng,Leshuai W Zhang,Roland H Stauber,Yong Wang,Mingyuan Gao

BioMed research international 2023:9466110 PubMed36820222

2023

Effect of Alleviating Fibrosis with EGCG-Modified Bone Graft in Murine Model Depended on Less Accumulation of Inflammatory Macrophage.

Applications

Unspecified application

Species

Unspecified reactive species

Dengbo Yao,Jiang Guo,Tianyu Qin,Haibao Chen,Song Jin

PLoS genetics 18:e1010510 PubMed36477146

2022

FgSnt1 of the Set3 HDAC complex plays a key role in mediating the regulation of histone acetylation by the cAMP-PKA pathway in Fusarium graminearum.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Gong,Daiying Xu,Daiyuan Sun,Jiangang Kang,Wei Wang,Jin-Rong Xu,Xue Zhang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e2105650 PubMed35603963

2022

Hybrid Biomaterial Initiates Refractory Wound Healing via Inducing Transiently Heightened Inflammatory Responses.

Applications

Unspecified application

Species

Unspecified reactive species

Xuemei Liu,Geng Dou,Zihan Li,Xiangdong Wang,Ronghua Jin,Yao Liu,Huijuan Kuang,Xiaoyao Huang,Xiaoxue Yang,Xiaoshan Yang,Siying Liu,Meiling Wu,Hao Guo,Feng Ding,Haokun Xu,Shiyu Liu,Yan Jin,Kun Xuan

Journal of leukocyte biology 112:9-21 PubMed34585429

2021

Mechanism of chlorogenic acid in alveolar macrophage polarization in Klebsiella pneumoniae-induced pneumonia.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Rong Li,Shi Rui Tan,Lu Yang,Wei He,Li Chen,Fen Xiu Shen,Zhuo Wang,Hai Feng Wang

BMC musculoskeletal disorders 22:494 PubMed34049522

2021

Changes in macrophage and inflammatory cytokine expressions during fracture healing in an ovariectomized mice model.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Chen,Shao Cheng,Kanghui Sun,Jing Wang,Xinhua Liu,Yongjian Zhao,Junjie Yang,Dongfeng Zhao,Chunchun Xue,Yuren Tao,Shitian Zhao,Hao Zhang,Sheng Lu,Qi Shi,Yongjun Wang,Bing Shu

Acta pharmaceutica Sinica. B 11:1069-1082 PubMed33996418

2021

Intracellular aggregation of peptide-reprogrammed small molecule nanoassemblies enhances cancer chemotherapy and combinatorial immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Jinrong Peng,Yao Xiao,Qian Yang,Qingya Liu,Yu Chen,Kun Shi,Ying Hao,Ruxia Han,Zhiyong Qian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-iNOS antibody [EPR16635]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-iNOS antibody [EPR16635] (AB209027)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Product promise