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AB200516

Alexa Fluor® 647 Anti-JunB antibody [EPR6518]

5

(5 Reviews)

|

(2 Publications)

Rabbit Recombinant Monoclonal JUNB antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

Transcription factor JunB, Transcription factor AP-1 subunit JunB, JUNB

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-JunB antibody [EPR6518] (AB200516)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-JunB antibody [EPR6518] (AB200516)

ab200516 staining JunB in MCF7 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200516 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2μg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6518

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The JunB protein also known as Jun B proto-oncogene is a transcription factor belonging to the AP-1 family. It has a molecular mass of approximately 36 kDa. JunB is expressed in various tissues including epithelial endothelial and immune cells. It often forms heterodimers with other proteins like c-Fos enhancing its capacity to regulate gene expression. Its expression levels can fluctuate with physiological changes and cellular stress indicating its role in managing dynamic cellular processes.
Biological function summary

JunB influences cell proliferation differentiation and apoptosis. As part of the AP-1 transcription factor complex it binds to specific DNA sequences regulating genes involved in these important processes. This ability to influence cell cycle and immune response highlights the importance of JunB in tissue development and function. Additionally JunB plays a role in controlling the expression of genes involved in inflammatory responses impacting immune cell activation and function.

Pathways

JunB interacts with various signaling cascades such as the MAPK/ERK and JAK/STAT pathways. These pathways are fundamental in transmitting extracellular signals to the nucleus affecting gene transcription. In the MAPK/ERK pathway JunB works closely with proteins such as c-Jun and c-Fos facilitating cell proliferation and survival. Furthermore it contributes to the JAK/STAT pathway by modulating the inflammatory response demonstrating JunB's adaptability across different cellular contexts.

Altered JunB expression links to conditions like cancer and autoimmune diseases. In some cancers such as leukemia aberrant levels of JunB disrupt normal cell growth collaborating with proteins like c-Myc to promote oncogenesis. In autoimmune disorders JunB can modify immune cell activity through proteins like STAT3 potentially exacerbating inflammation. Understanding JunB functions in these contexts provides insights into potential therapeutic targets for these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[GC]TCA-3'. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to an AP-1 consensus sequence and its transcriptional activity (By similarity).
See full target information Transcription factor jun-B
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:13390 PubMed38862642

2024

Identification of a risk model for prognostic and therapeutic prediction in renal cell carcinoma based on infiltrating M0 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyong Xin,Junjie Su,Ruixin Li,Qiong Cao,Haojie Wang,Zhihao Wei,Chengliang Wang,Chengdong Zhang

Developmental cell 52:779-793.e7 PubMed32059774

2020

Sphingosine 1-Phosphate Receptor Signaling Establishes AP-1 Gradients to Allow for Retinal Endothelial Cell Specialization.

Applications

Unspecified application

Species

Unspecified reactive species

Keisuke Yanagida,Eric Engelbrecht,Colin Niaudet,Bongnam Jung,Konstantin Gaengel,Kristina Holton,Steven Swendeman,Catherine H Liu,Michel V Levesque,Andrew Kuo,Zhongjie Fu,Lois E H Smith,Christer Betsholtz,Timothy Hla
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com