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Mouse Recombinant Monoclonal Ki67 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Ki67 antibody [B56] (AB283699), expandable thumbnail
  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Ki67 antibody [B56] (AB283699), expandable thumbnail
  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Ki67 antibody [B56] (AB283699), expandable thumbnail

Key facts

Isotype
IgG
Host species
Mouse
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow Cyt (Intra)
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Tested
Tested

Species
Human
Dilution info
1/5000
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

Target data

Function

The protein expressed by the MKI67 gene is required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly and associates with the surface of the mitotic chromosome, specifically the perichromosomal layer, covering a significant portion of the chromosome surface (PubMed:27362226). It prevents chromosomes from collapsing into a single chromatin mass by creating a steric and electrostatic charge barrier due to its high net electrical charge, acting as a surfactant that disperses chromosomes and enables their independent motility (PubMed:27362226). The protein binds DNA, with a preference for supercoiled and AT-rich DNA (PubMed:10878551), and may play a role in chromatin organization, though it is unclear if it directly influences chromatin organization or if this is an indirect result of its function in maintaining dispersed mitotic chromosomes (Probable, PubMed:24867636). This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67

Recommended products

Mouse Recombinant Monoclonal Ki67 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
B56
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Ki67 is a nuclear protein often referred to as MKI67 with a molecular weight of approximately 345 kDa. This protein is strongly associated with cell proliferation. Scientists commonly use Ki67 staining techniques to identify and quantify proliferating cells in tissues. Ki67 is expressed in the nucleus during active cell cycle phases—G1 S G2 and M—but not in resting cells in G0 phase. It is detectable through methods such as Ki67 immunofluorescence and Ki67 flow cytometry which are important for analyzing cell division.

Biological function summary

The Ki67 protein plays an essential role in cellular proliferation processes. It does not form part of a stable complex but interacts transiently with other cell cycle-related proteins. Research indicates that Ki67 maintains the structure of the perichromosomal layer during mitosis impacting chromosome separation. It is particularly active in tissues with high cell turnover such as bone marrow and lymphoid organs.

Pathways

Ki67 influences several key cellular pathways that control cell proliferation and differentiation. The protein acts within the regulation of the cell cycle and the PI3K/AKT signaling pathway important for cell growth and survival. Within these pathways Ki67 interacts with proteins like cyclin-dependent kinases (CDKs) which regulate transitions between different phases of the cell cycle.

Associated diseases and disorders

Ki67 has significant implications in oncology and can be used as a biomarker for cancer prognosis. Its expression levels help determine the aggressiveness of tumors especially in breast cancer and prostate cancer. High Ki67 levels correlate with poor prognosis as it indicates rapid cell division. In cancer Ki67 associates with p53 a protein that regulates the cell cycle and function as a tumor suppressor.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Ki67 antibody [B56] (ab283699), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Ki67 antibody [B56] (ab283699)

    Immunofluorescent analysis of 4% Paraformaldehyde HeLa (human cervix adenocarcinoma epithelial cells) labelling Ki67 with ab283699 at 1/500 dilution, (Red). Confocal image showing nucleolar staining on HeLa cells. Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 Anti-beta Tubulin rabbit monoclonal antibody - Microtubule Marker was used to counterstain tubulin at 1/200 dilution. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was used as the counterstain secondary antibody at 1/1000 (Green). The nuclear counterstain was DAPI (Blue).

  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Ki67 antibody [B56] (ab283699), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Ki67 antibody [B56] (ab283699)

    Intracellular Flow cytometric scatterplot showing 4% paraformaldehyde fixed HeLa (Human cervix adenocarcinoma epithelial cells) permeabilized with 90% methanol labelling Ki67 with ab283699 at 1/5000 dilution (Red). Mouse monoclonal IgG (Left)

    Cells were co-stained with DAPI to differentiate cell cycle phase.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Ki67 antibody [B56] (ab283699), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Ki67 antibody [B56] (ab283699)

    Intracellular Flow cytometric overlay histogram showing 4% paraformaldehyde fixed HeLa (Human cervix adenocarcinoma epithelial cells (Right)) permeabilized with 90% methanol labelling Ki67 with ab283699 at 1/5000 dilution (Red). MKI67 KO HeLa (Left). Isotype control was Mouse monoclonal IgG (Black). Unlabelled control: Cells without incubation with primary antibody and secondary antibody (Blue).

    Positive staining on HeLa cells (ab255448), while no staining on Ki67 knockout HeLa cells (Human MKI67 (Ki67) knockout HeLa cell line ab255407).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com