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AB196907

Alexa Fluor® 647 Anti-Ki67 antibody [EPR3610]

5

(1 Review)

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(4 Publications)

Anti-Ki67 antibody [EPR3610] - Alexa Fluor® 647 conjugated (ab196907) is a rabbit recombinant monoclonal antibody detecting Ki67 in ICC/IF. Suitable for Human.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67

3 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Ki67 antibody [EPR3610] (AB196907)
  • ICC/IF

AbReview81456****

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Ki67 antibody [EPR3610] (AB196907)

Immunocytochemistry analysis of buffered formalin-fixed 0.05% Tween 20 permeabilized human rectal cancer spheroids, SW837 staining with ab196907 at 1/100 dilution. Samples were incubated with the primary antibody with Blocking solution for 12 hours at 4°C. Blocking was done using 1% BSA + 10% FBS for 1 hour at 25°C.

This image is courtesy of an Abreview submitted by Tania Cruz

Immunocytochemistry - Alexa Fluor® 647 Anti-Ki67 antibody [EPR3610] (AB196907)
  • ICC

Lab

Immunocytochemistry - Alexa Fluor® 647 Anti-Ki67 antibody [EPR3610] (AB196907)

ab196907 staining Ki67 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab196907 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution, overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Ki67 antibody [EPR3610] (AB196907)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Ki67 antibody [EPR3610] (AB196907)

ab196907 staining Ki67 in wild-type HAP1 cells (top panel) and Ki67 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab196907 at 1/100 dilution (shown in red) and ab195887 at 1/250 dilution (shown in green) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3610

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Human

Applications

Flow Cyt (Intra), ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>If fixing cells in 4% PFA, it is recommended to permeabilized cells with 0.1% Triton-X for 5 min.</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Alexa Fluor® 647 Anti-Ki67 antibody [EPR3610] (ab196907) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Related products
Antibody clone EPR3610 is also available pre-conjugated to a variety of labels for your convenience – Anti-Ki67 Alexa Fluor® 647 [EPR3610] (ab196907).

Specificity confirmed
The specificity of Alexa Fluor® 647 Anti-Ki67 antibody [EPR3610] (ab196907) has been confirmed by Immunocytochemistry testing in MKI67 Knockout HAP1 cells.

Other related products
We have a range of other formats of antibody clone [EPR3610] also available for your convenience: ab92742, Alexa Fluor® 647 - ab196907, Alexa Fluor® 488 - ab197234, Carrier free - ab209897, Alexa Fluor® 568 - ab211968, HRP - ab212215, Alexa Fluor® 555 - ab215226, Alexa Fluor® 594 - ab216709, APC - ab310886, PE - ab310942, Alexa Fluor® 750 - ab321771

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ki67 is a nuclear protein often referred to as MKI67 with a molecular weight of approximately 345 kDa. This protein is strongly associated with cell proliferation. Scientists commonly use Ki67 staining techniques to identify and quantify proliferating cells in tissues. Ki67 is expressed in the nucleus during active cell cycle phases—G1 S G2 and M—but not in resting cells in G0 phase. It is detectable through methods such as Ki67 immunofluorescence and Ki67 flow cytometry which are important for analyzing cell division.
Biological function summary

The Ki67 protein plays an essential role in cellular proliferation processes. It does not form part of a stable complex but interacts transiently with other cell cycle-related proteins. Research indicates that Ki67 maintains the structure of the perichromosomal layer during mitosis impacting chromosome separation. It is particularly active in tissues with high cell turnover such as bone marrow and lymphoid organs.

Pathways

Ki67 influences several key cellular pathways that control cell proliferation and differentiation. The protein acts within the regulation of the cell cycle and the PI3K/AKT signaling pathway important for cell growth and survival. Within these pathways Ki67 interacts with proteins like cyclin-dependent kinases (CDKs) which regulate transitions between different phases of the cell cycle.

Ki67 has significant implications in oncology and can be used as a biomarker for cancer prognosis. Its expression levels help determine the aggressiveness of tumors especially in breast cancer and prostate cancer. High Ki67 levels correlate with poor prognosis as it indicates rapid cell division. In cancer Ki67 associates with p53 a protein that regulates the cell cycle and function as a tumor suppressor.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein that associates with the surface of mitotic chromosomes and acts both as a chromosome repellent during early mitosis and chromosome attractant during late mitosis (PubMed : 27362226, PubMed : 32879492, PubMed : 35513709, PubMed : 39153474). Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed : 27362226). During early mitosis, relocalizes from nucleoli to the chromosome surface where it forms extended brush structures that cover a substantial fraction of the chromosome surface (PubMed : 27362226). The MKI67 brush structure prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier : the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed : 27362226). During mitotic anaphase, the MKI67 brush structure collapses and MKI67 switches from a chromosome repellent to a chromosome attractant to promote chromosome clustering and facilitate the exclusion of large cytoplasmic particles from the future nuclear space (PubMed : 32879492, PubMed : 39153474). Mechanistically, dephosphorylation during mitotic exit and simultaneous exposure of a conserved basic patch induce the RNA-dependent formation of a liquid-like condensed phase on the chromosome surface, promoting coalescence of neighboring chromosome surfaces and clustering of chromosomes (PubMed : 39153474). Binds premature ribosomal RNAs during anaphase; promoting liquid-liquid phase separation (PubMed : 28935370, PubMed : 39153474). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed : 10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization; it is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in mitotic chromosome (PubMed : 24867636).
See full target information MKI67
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:630 PubMed39805872

2025

Increased SOAT2 expression in aged regulatory T cells is associated with altered cholesterol metabolism and reduced anti-tumor immunity.

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Mingjiong Zhang,Jiahua Cui,Haoyan Chen,Yifan Cheng,Qiaoyu Chen,Feng Zong,Xiao Lu,Lang Qin,Yu Han,Xingwang Kuai,Yuxing Zhang,Minjie Chu,Shuangshuang Wu,Jianqing Wu

Communications biology 4:1001 PubMed34429505

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Tudor Petreus,Elaine Cadogan,Gareth Hughes,Aaron Smith,Venkatesh Pilla Reddy,Alan Lau,Mark James O'Connor,Susan Critchlow,Marianne Ashford,Lenka Oplustil O'Connor

Experimental and therapeutic medicine 21:443 PubMed33747179

2021

Oleanolic acid inhibits osteosarcoma cell proliferation and invasion by suppressing the SOX9/Wnt1 signaling pathway.

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Xianming Chen,Yulin Zhang,Sen Zhang,Aiming Wang,Quanyin Du,Ziming Wang

Scientific reports 7:6519 PubMed28747680

2017

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Cassandra L Pegg,Leanne T Cooper,Jing Zhao,Michael Gerometta,Fiona M Smith,Michael Yeh,Perry F Bartlett,Jeffrey J Gorman,Andrew W Boyd
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