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AB314812

Alexa Fluor® 647 Anti-KLRF1 antibody [EPR23633-150]

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Rabbit Recombinant Monoclonal KLRF1 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CLEC5C, ML, KLRF1, Killer cell lectin-like receptor subfamily F member 1, Lectin-like receptor F1, Activating coreceptor NKp80, C-type lectin domain family 5 member C

1 Images
Flow Cytometry - Alexa Fluor® 647 Anti-KLRF1 antibody [EPR23633-150] (AB314812)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Anti-KLRF1 antibody [EPR23633-150] (AB314812)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling KLRF1 with ab314812 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Gated on viable cells. Cells are co-stained with CD56 conjugated with Brilliant Violet 421.

  • 578 PE

    PE Anti-KLRF1 antibody [EPR23633-150]

  • Unconjugated

    Anti-KLRF1 antibody [EPR23633-150]

  • Carrier free

    Anti-KLRF1 antibody [EPR23633-150] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23633-150

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLRF1 also known as CD314 or NKp80 is a receptor protein found primarily on natural killer (NK) cells with a significant mass of about 30 kDa. Researchers have identified its expression mainly in tissues rich in NK cells such as the spleen and peripheral blood. This receptor structurally belongs to the C-type lectin-like domain family and plays an important role in the immune response by binding to the CLEC2B ligand on target cells facilitating NK cell activation and cytotoxicity.
Biological function summary

In immune surveillance and homeostasis KLRF1 acts as an important activating receptor on natural killer cells. It does not form a complex but works independently to control cellular immune responses by recognizing stress-induced ligands on target cells. Upon interaction with its ligand KLRF1 triggers a cascade of intracellular signals that promote cytotoxic activity and cytokine secretion contributing to the elimination of virally infected or transformed cells.

Pathways

In the context of innate immune response signaling KLRF1 interacts with pathways involving NK cell-mediated cytotoxicity. KLRF1 engagement stimulates pathways similar to those activated by other receptors in the natural killer cell repertoire such as KLRK1 (NKG2D). These interactions play roles in maintaining balance within the immune system by modulating NK cell activity and ensuring efficient target cell elimination.

KLRF1 has implications in immune system-related conditions such as cancers and viral infections. Altered expression or function of KLRF1 may contribute to the escape of tumor cells from immune surveillance or decreased viral clearance. Associations with proteins like KLRD1 (CD94) in certain hematological malignancies suggest a role for KLRF1 in disease pathogenesis where NK cell dysfunction is present underlining its significance in developing therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as an activating receptor involved in immunosurveillance upon binding to various ligands displayed at the surface of myeloid cells. Upon interaction with CLEC2B ligand, stimulates NK-cell cytotoxicity and cytokine production leading to the cytolysis of malignant CLEC2B-expressing myeloid cells. Actviation of the common cytotoxicity pathway involves SRC and SYK kinases (PubMed : 21149606).
See full target information KLRF1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com